| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,422,503 | A→G | 7.4% | V71V (GTA→GTG) | yhdZ → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,422,503 | 0 | A | G | 7.4% | 221.9 / 6.9 | 81 | V71V (GTA→GTG) | yhdZ | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (21/54); new base G (3/3); total (24/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CCGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCTTT > W3110S.gb/3422436‑3422572 | ccGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTGGa < 1:2561709/70‑1 (MQ=255)ccGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTGGa < 1:3609911/70‑1 (MQ=255)ccGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGAt > 1:1948784/1‑71 (MQ=255) cGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGAt < 1:2410949/70‑1 (MQ=255) tAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCa > 1:1031615/1‑71 (MQ=255) tAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCa > 1:920287/1‑71 (MQ=255) tAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCa > 1:3593483/1‑71 (MQ=255) aCAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACt < 1:3252090/71‑1 (MQ=255) cAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACtt < 1:226864/71‑1 (MQ=255) aaCCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGa > 1:2108419/1‑57 (MQ=255) aaCCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGa > 1:2157903/1‑57 (MQ=255) aaCCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGTCATCGa < 1:2555715/66‑1 (MQ=255) aaCCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGa < 1:2530543/66‑1 (MQ=255) aaCCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTa < 1:1994850/71‑1 (MQ=255) ttCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:2303625/71‑1 (MQ=255) ttCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:1766012/71‑1 (MQ=255) cGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATg > 1:2413243/1‑67 (MQ=255) cGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAg < 1:946452/70‑1 (MQ=255) agTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATg > 1:3026532/2‑67 (MQ=255) gTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGat < 1:2329661/71‑1 (MQ=255) gTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGa > 1:2096361/1‑70 (MQ=255) gTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGaa > 1:3517097/1‑56 (MQ=255) tATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGa < 1:2759831/45‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACtt < 1:2758850/56‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACtt < 1:1785747/56‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:2248647/62‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:10074/62‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:189242/62‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:401810/62‑1 (MQ=255) ttAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCg > 1:475866/1‑70 (MQ=255) aaTCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCaa > 1:1920391/1‑71 (MQ=255) tcatcTGGAAGAACATCAACAGGGACGGATCGTGGTAg < 1:3854530/38‑1 (MQ=255) tcatcTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAg < 1:2794350/59‑1 (MQ=255) tcatcTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAg < 1:863580/59‑1 (MQ=255) atcTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAatat < 1:1479910/70‑1 (MQ=255) tcTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGa < 1:1764025/54‑1 (MQ=255) cTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATAtt > 1:1377955/1‑69 (MQ=255) tGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa > 1:3125832/1‑53 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATgg < 1:3496503/35‑1 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATTGCATCGAACTTAATGAAGATATCCGCAATATTGAg < 1:2582064/69‑1 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAAt < 1:1592880/48‑1 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAAt < 1:646800/48‑1 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTg < 1:1796549/67‑1 (MQ=255) gaagaaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAg < 1:3124357/69‑1 (MQ=255) gaaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGt < 1:3188362/71‑1 (MQ=255) aaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTc < 1:2501716/71‑1 (MQ=255) aaCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTc < 1:412313/71‑1 (MQ=255) aCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGaa < 1:1549691/46‑1 (MQ=255) aCATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAg > 1:3793378/1‑47 (MQ=255) aTCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGa > 1:934552/1‑46 (MQ=255) aTCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGa > 1:1027734/1‑46 (MQ=255) aTCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGc < 1:3042676/53‑1 (MQ=255) tCAACAGGGACGGATCGTGGTGGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTcagg > 1:3659699/1‑70 (MQ=255) tCAACAGGGACGGATCGTGGTGGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTcagg > 1:2665004/1‑70 (MQ=255) tCAACAGGGACGGATCGTGGTGGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTcagg > 1:1872358/1‑70 (MQ=255) tCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGa < 1:987371/45‑1 (MQ=255) tCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTg < 1:3044763/59‑1 (MQ=255) cAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGa < 1:1929275/44‑1 (MQ=255) aaCAGGGACGGATCGTGGTAGATGGCATCGAACTTAAt > 1:2951712/1‑38 (MQ=255) aaCAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATcc > 1:2040639/1‑48 (MQ=255) cAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGc < 1:817704/48‑1 (MQ=255) cAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGc < 1:1613118/48‑1 (MQ=255) cAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGa < 1:1502535/71‑1 (MQ=255) aGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGat < 1:473239/71‑2 (MQ=255) aGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGaa < 1:860790/71‑1 (MQ=255) gggACGGATCGTGGTGGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTcaggcagg < 1:2386512/68‑1 (MQ=255) gggACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATcc < 1:1976978/44‑1 (MQ=255) gggACGGATCGTGGTAGATGGCATCGAAATTAATGAAGATATcc < 1:1394648/44‑1 (MQ=255) cGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTcaggcagg < 1:1374445/64‑1 (MQ=255) cGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGCCAGGAAGTggg < 1:1209900/71‑1 (MQ=255) ggATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCa < 1:3575159/42‑1 (MQ=255) gATCGTGGTAGATGTCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGaa < 1:1275649/71‑1 (MQ=255) aTCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAAt < 1:734597/71‑1 (MQ=255) aTCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAAt < 1:783259/71‑1 (MQ=255) cgaggTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAAt > 1:1690505/4‑69 (MQ=255) cGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAAt > 1:3827289/1‑69 (MQ=255) cGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATg < 1:1121956/70‑1 (MQ=255) gTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATgg < 1:1603919/70‑1 (MQ=255) gTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCtt < 1:1448506/71‑1 (MQ=255) tAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCttt < 1:1419897/71‑1 (MQ=255) tAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCtt < 1:548060/70‑1 (MQ=255) aGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCttt > 1:787575/1‑70 (MQ=255) aGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCttt > 1:1215610/1‑70 (MQ=255) | CCGGTAAATCGACAACCATTCGTTGTATTAATCATCTGGAAGAACATCAACAGGGACGGATCGTGGTAGATGGCATCGAACTTAATGAAGATATCCGCAATATTGAGCGCGTCAGGCAGGAAGTGGGAATGGTCTTT > W3110S.gb/3422436‑3422572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |