| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,440,923 | T→C | 19.2% | S114S (AGT→AGC) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,440,923 | 0 | T | C | 19.2% | 48.9 / 7.1 | 26 | S114S (AGT→AGC) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (13/8); new base C (3/2); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTAC > W3110S.gb/3440857‑3440987 | gCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCg < 1:1737983/71‑1 (MQ=255) gCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGAt < 1:211067/69‑1 (MQ=255) gCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTa > 1:1056505/1‑71 (MQ=255) cAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGAt < 1:534710/68‑1 (MQ=255) cAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGAt < 1:1587239/68‑1 (MQ=255) gTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTgg > 1:2404583/1‑71 (MQ=255) gTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTgg > 1:1544631/1‑71 (MQ=255) gATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTgg > 1:1641230/1‑68 (MQ=255) tCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAgat < 1:65014/71‑1 (MQ=255) cGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGCTCCGATTACACTAAAGCGCGGCTGGCAgat > 1:1778768/1‑70 (MQ=255) cGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGCTCCGATTACACTAAAGCGCGGCTGGCAgat > 1:726371/1‑70 (MQ=255) cGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGCTCCGATTACACTAAAGCGCGGCTGGCAgat > 1:2870123/1‑70 (MQ=255) gATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAgatga < 1:2390447/71‑1 (MQ=255) gATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAgatga < 1:3436215/71‑1 (MQ=255) gATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGCTCCGATTACACTAAAGCGCGGCTGGCAgatga < 1:1016240/71‑1 (MQ=255) cgcgcgGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGc > 1:1572641/1‑71 (MQ=255) cgcgcgGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGc > 1:337449/1‑71 (MQ=255) gaagaaCTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGaa > 1:3027534/1‑66 (MQ=255) gaagaaCTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCg > 1:3385912/1‑71 (MQ=255) agaaCTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTTGCAGATGATGGCCTCGACGAACTGCGtt > 1:295235/1‑71 (MQ=255) gaaCTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGttt > 1:35898/1‑71 (MQ=255) aaCTTACCGTGCGCAGCTCCGATTACACTAAAGCGCGGCTGGCAgatga < 1:1606401/49‑1 (MQ=255) aCTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGtttt < 1:3846827/70‑1 (MQ=255) cTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAg > 1:801672/1‑71 (MQ=255) gTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGatgatg > 1:2161135/1‑43 (MQ=255) cgcAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTAc > 1:1786643/1‑70 (MQ=255) | GCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTAC > W3110S.gb/3440857‑3440987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |