| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,448,059 | T→C | 10.0% | P1091P (CCA→CCG) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,448,059 | 0 | T | C | 10.0% | 101.1 / 3.3 | 40 | P1091P (CCA→CCG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/22); new base C (2/2); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
CTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCTTTACCA > W3110S.gb/3447990‑3448122 | cTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTg > 1:276477/1‑71 (MQ=255) cATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGa < 1:56073/71‑1 (MQ=255) ttCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgt > 1:1983632/1‑71 (MQ=255) aGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGtt < 1:1324581/71‑1 (MQ=255) aaCAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTAcc < 1:2351019/69‑1 (MQ=255) gCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTg < 1:1096963/43‑1 (MQ=255) gCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTg < 1:1238989/43‑1 (MQ=255) gCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTg < 1:810949/43‑1 (MQ=255) ttACCCGGCAGGAAGTACTGCGCAGGCATATCGGTACCCGGGATCAGAACGTCGTTACCCTGAGCATCAAc > 1:378391/1‑71 (MQ=255) ttACCCGGCAGGAAGTACTGCGCAGGCATATCGGTACCCGGGATCAGAACGTCGTTACCCTGAGCATCAAc > 1:3346083/1‑71 (MQ=255) cccGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGa < 1:1524477/70‑1 (MQ=255) ggCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGAtttt < 1:3084531/71‑1 (MQ=255) ggCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGAtttt < 1:2233277/71‑1 (MQ=255) gCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTc < 1:539980/71‑1 (MQ=255) catgAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTc < 1:1989379/67‑1 (MQ=255) aGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAg < 1:194173/71‑1 (MQ=255) gAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTAc > 1:3600450/1‑47 (MQ=255) gAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTg > 1:1026333/1‑71 (MQ=255) aaGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGc > 1:2751974/1‑71 (MQ=255) aaGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGc > 1:1918518/1‑71 (MQ=255) aGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGcc > 1:2315767/1‑71 (MQ=255) cTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGa < 1:1538269/56‑1 (MQ=255) cTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGa < 1:2929468/56‑1 (MQ=255) cgcTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAg > 1:1719748/1‑60 (MQ=255) cgcTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACg < 1:2958797/69‑1 (MQ=255) gcaggCATATCGGTACCCGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAg < 1:2014990/68‑1 (MQ=255) gcaggCATATCGGTACCCGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAg < 1:3267181/68‑1 (MQ=255) gCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCaa > 1:3065006/1‑45 (MQ=255) gCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATc < 1:972907/70‑1 (MQ=255) cATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCtt < 1:1870925/71‑1 (MQ=255) tatCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAg < 1:664155/64‑1 (MQ=255) atCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGAtttt < 1:1743385/48‑1 (MQ=255) atCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGAtttt < 1:1516023/48‑1 (MQ=255) tCGGTACCTGTGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAg < 1:1189649/62‑1 (MQ=255) tCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAAc > 1:1031921/1‑41 (MQ=255) tCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAAc > 1:1980670/1‑41 (MQ=255) tCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCttt < 1:3797061/68‑1 (MQ=255) cGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCTTTacca > 1:1152929/1‑71 (MQ=255) ggTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCTTTac > 1:2076391/1‑68 (MQ=255) ggTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCTTTac > 1:1383781/1‑68 (MQ=255) | CTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCAACGATTTTCAGTGCCGGACGCAGATCTTTACCA > W3110S.gb/3447990‑3448122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |