| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,521,980 | C→T | 6.0% | G293G (GGC→GGT) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,521,980 | 0 | C | T | 6.0% | 257.2 / 3.9 | 83 | G293G (GGC→GGT) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base C (44/34); new base T (2/3); total (46/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
CGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCC > W3110S.gb/3521911‑3522050 | cGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCa < 1:2771534/71‑1 (MQ=255) atatGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCAt > 1:3712521/1‑70 (MQ=255) atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg > 1:208758/1‑71 (MQ=255) atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg > 1:956744/1‑71 (MQ=255) atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg > 1:849506/1‑71 (MQ=255) tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc > 1:2581002/1‑71 (MQ=255) tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc > 1:2783419/1‑71 (MQ=255) tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc > 1:3831803/1‑71 (MQ=255) tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc > 1:506287/1‑71 (MQ=255) cgcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaa < 1:1259777/71‑1 (MQ=255) gcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa > 1:3625107/1‑69 (MQ=255) gcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa > 1:486494/1‑69 (MQ=255) cAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAg < 1:2232685/71‑1 (MQ=255) gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAgg < 1:838221/70‑1 (MQ=255) gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGc < 1:171257/71‑1 (MQ=255) gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGc < 1:1714790/71‑1 (MQ=255) cGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCa < 1:2412964/71‑1 (MQ=255) gATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa < 1:1796178/71‑1 (MQ=255) aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGTCATTAGCCGCAAAGGCAat < 1:1687534/71‑1 (MQ=255) aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCAt < 1:263264/55‑1 (MQ=255) aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAat < 1:148842/71‑1 (MQ=255) aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAat < 1:2790651/71‑1 (MQ=255) tAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAata < 1:2396900/71‑1 (MQ=255) aCGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAatat > 1:3121244/1‑70 (MQ=255) gTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc < 1:2428173/71‑1 (MQ=255) aTCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGAc > 1:3143220/1‑71 (MQ=255) tcttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa < 1:1060048/61‑1 (MQ=255) cttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTa > 1:1980528/1‑71 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACt < 1:2761355/67‑1 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc > 1:1859000/1‑70 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc > 1:313996/1‑70 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAc > 1:2936784/1‑69 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc < 1:3793209/60‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc < 1:1682923/60‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:2293034/69‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:1810708/69‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:293348/69‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:2926464/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:3123998/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:15553/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg < 1:1944235/71‑1 (MQ=255) ccGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa < 1:561820/42‑1 (MQ=255) ccGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa < 1:3654413/49‑1 (MQ=255) cGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaaa > 1:3537561/1‑43 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaaa > 1:1340991/1‑42 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:689664/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:2831506/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:1569103/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa > 1:970378/1‑64 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa > 1:3049543/1‑64 (MQ=255) gTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg < 1:2687444/65‑1 (MQ=255) tATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa < 1:1158909/38‑1 (MQ=255) tATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa < 1:3406515/38‑1 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAgg > 1:1430918/1‑38 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa > 1:3690814/1‑41 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:1836547/1‑49 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACgctgct > 1:1444274/1‑65 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:1348449/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3045977/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:750130/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3211166/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:1545798/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:2957595/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3100481/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCAGCAAAGGCAATATCGCGa > 1:771795/1‑49 (MQ=255) aCCAAAAGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:494970/1‑71 (MQ=255) ccAAAGGCGATCTGCTGGAAGGTATTGGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt > 1:1502218/1‑70 (MQ=255) ccAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt > 1:2431251/1‑70 (MQ=255) ccAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg < 1:229439/70‑1 (MQ=255) cAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa < 1:1635564/39‑1 (MQ=255) aaaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg > 1:3802691/1‑71 (MQ=255) aaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg > 1:1343771/1‑70 (MQ=255) aGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGc > 1:675117/1‑70 (MQ=255) tgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg > 1:3140412/1‑70 (MQ=255) ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg < 1:380177/68‑1 (MQ=255) ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGc < 1:3243876/69‑1 (MQ=255) gAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCa < 1:113803/70‑1 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc < 1:469113/71‑1 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:1810613/1‑71 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:257430/1‑71 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg > 1:495167/1‑71 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg < 1:376657/71‑1 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg > 1:2745108/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:779750/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:826038/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:2657634/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:3231192/1‑70 (MQ=255) | CGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCC > W3110S.gb/3521911‑3522050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |