Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,521,980 C→T 6.0% G293G (GGC→GGT glpX → fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,521,9800CT 6.0% 257.2 / 3.9 83G293G (GGC→GGTglpXfructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base C (44/34);  new base T (2/3);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

CGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCC  >  W3110S.gb/3521911‑3522050
                                                                     |                                                                      
cGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCa                                                                       <  1:2771534/71‑1 (MQ=255)
  atatGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCAt                                                                      >  1:3712521/1‑70 (MQ=255)
    atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg                                                                   >  1:208758/1‑71 (MQ=255)
    atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg                                                                   >  1:956744/1‑71 (MQ=255)
    atGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAg                                                                   >  1:849506/1‑71 (MQ=255)
     tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc                                                                  >  1:2581002/1‑71 (MQ=255)
     tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc                                                                  >  1:2783419/1‑71 (MQ=255)
     tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc                                                                  >  1:3831803/1‑71 (MQ=255)
     tGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGc                                                                  >  1:506287/1‑71 (MQ=255)
          cgcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaa                                                             <  1:1259777/71‑1 (MQ=255)
           gcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa                                                              >  1:3625107/1‑69 (MQ=255)
           gcAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa                                                              >  1:486494/1‑69 (MQ=255)
            cAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAg                                                           <  1:2232685/71‑1 (MQ=255)
              gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAgg                                                          <  1:838221/70‑1 (MQ=255)
              gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGc                                                         <  1:171257/71‑1 (MQ=255)
              gCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGc                                                         <  1:1714790/71‑1 (MQ=255)
               cGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCa                                                        <  1:2412964/71‑1 (MQ=255)
                gATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa                                                       <  1:1796178/71‑1 (MQ=255)
                 aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGTCATTAGCCGCAAAGGCAat                                                      <  1:1687534/71‑1 (MQ=255)
                 aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCAt                                                                      <  1:263264/55‑1 (MQ=255)
                 aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAat                                                      <  1:148842/71‑1 (MQ=255)
                 aTAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAat                                                      <  1:2790651/71‑1 (MQ=255)
                  tAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAata                                                     <  1:2396900/71‑1 (MQ=255)
                    aCGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAatat                                                    >  1:3121244/1‑70 (MQ=255)
                      gTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc                                                 <  1:2428173/71‑1 (MQ=255)
                         aTCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGAc                                              >  1:3143220/1‑71 (MQ=255)
                          tcttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa                                                       <  1:1060048/61‑1 (MQ=255)
                           cttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTa                                            >  1:1980528/1‑71 (MQ=255)
                              ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACt                                             <  1:2761355/67‑1 (MQ=255)
                              ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc                                          >  1:1859000/1‑70 (MQ=255)
                              ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc                                          >  1:313996/1‑70 (MQ=255)
                              ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAc                                           >  1:2936784/1‑69 (MQ=255)
                                 tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc                                                 <  1:3793209/60‑1 (MQ=255)
                                 tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATcgc                                                 <  1:1682923/60‑1 (MQ=255)
                                 tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa                                        <  1:2293034/69‑1 (MQ=255)
                                 tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa                                        <  1:1810708/69‑1 (MQ=255)
                                 tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa                                        <  1:293348/69‑1 (MQ=255)
                                   ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa                                       <  1:2926464/68‑1 (MQ=255)
                                   ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa                                       <  1:3123998/68‑1 (MQ=255)
                                   ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa                                       <  1:15553/68‑1 (MQ=255)
                                   ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg                                    <  1:1944235/71‑1 (MQ=255)
                                      ccGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa                                                              <  1:561820/42‑1 (MQ=255)
                                      ccGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa                                                       <  1:3654413/49‑1 (MQ=255)
                                       cGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaaa                                                            >  1:3537561/1‑43 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCaaa                                                            >  1:1340991/1‑42 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa                                               >  1:689664/1‑55 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa                                               >  1:2831506/1‑55 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa                                               >  1:1569103/1‑55 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa                                      >  1:970378/1‑64 (MQ=255)
                                        ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa                                      >  1:3049543/1‑64 (MQ=255)
                                         gTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg                                    <  1:2687444/65‑1 (MQ=255)
                                          tATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa                                                              <  1:1158909/38‑1 (MQ=255)
                                          tATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCa                                                              <  1:3406515/38‑1 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAgg                                                          >  1:1430918/1‑38 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa                                                       >  1:3690814/1‑41 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa                                               >  1:1836547/1‑49 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACgctgct                               >  1:1444274/1‑65 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:1348449/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:3045977/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:750130/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:3211166/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:1545798/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:2957595/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:3100481/1‑71 (MQ=255)
                                              aCCAAAGGCGATCTGCTGGAAGGCATTAGCAGCAAAGGCAATATCGCGa                                               >  1:771795/1‑49 (MQ=255)
                                              aCCAAAAGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         >  1:494970/1‑71 (MQ=255)
                                               ccAAAGGCGATCTGCTGGAAGGTATTGGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt                        >  1:1502218/1‑70 (MQ=255)
                                               ccAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt                        >  1:2431251/1‑70 (MQ=255)
                                               ccAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg                         <  1:229439/70‑1 (MQ=255)
                                                cAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCaa                                                       <  1:1635564/39‑1 (MQ=255)
                                                 aaaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg                      >  1:3802691/1‑71 (MQ=255)
                                                  aaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg                      >  1:1343771/1‑70 (MQ=255)
                                                   aGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGc                     >  1:675117/1‑70 (MQ=255)
                                                           tgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg             >  1:3140412/1‑70 (MQ=255)
                                                             ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg             <  1:380177/68‑1 (MQ=255)
                                                             ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGc            <  1:3243876/69‑1 (MQ=255)
                                                                gAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCa        <  1:113803/70‑1 (MQ=255)
                                                                  aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc     <  1:469113/71‑1 (MQ=255)
                                                                  aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc     >  1:1810613/1‑71 (MQ=255)
                                                                  aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc     >  1:257430/1‑71 (MQ=255)
                                                                   ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg    >  1:495167/1‑71 (MQ=255)
                                                                   ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg    <  1:376657/71‑1 (MQ=255)
                                                                   ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg    >  1:2745108/1‑71 (MQ=255)
                                                                     cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc  >  1:779750/1‑71 (MQ=255)
                                                                     cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc  >  1:826038/1‑71 (MQ=255)
                                                                     cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc  >  1:2657634/1‑71 (MQ=255)
                                                                     cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc   >  1:3231192/1‑70 (MQ=255)
                                                                     |                                                                      
CGATATGGCGCGCAGCGATAACGTCATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCC  >  W3110S.gb/3521911‑3522050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: