| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,522,004 | G→C | 6.8% | A301A (GCG→GCC) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,522,004 | 0 | G | C | 6.8% | 210.8 / 4.0 | 73 | A301A (GCG→GCC) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base G (42/26); new base C (2/3); total (44/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
ATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGG > W3110S.gb/3521936‑3522074 | aTCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGAc > 1:3143220/1‑71 (MQ=255) cttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTa > 1:1980528/1‑71 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACt < 1:2761355/67‑1 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc > 1:313996/1‑70 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAcc > 1:1859000/1‑70 (MQ=255) ctctGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTAc > 1:2936784/1‑69 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:293348/69‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:2293034/69‑1 (MQ=255) tGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGa < 1:1810708/69‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:2926464/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:3123998/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGaa < 1:15553/68‑1 (MQ=255) ccACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg < 1:1944235/71‑1 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:2831506/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:689664/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:1569103/1‑55 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa > 1:3049543/1‑64 (MQ=255) ggTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGaaa > 1:970378/1‑64 (MQ=255) gTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg < 1:2687444/65‑1 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGa > 1:1836547/1‑49 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACgctgct > 1:1444274/1‑65 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:1545798/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3045977/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3100481/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:2957595/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:3211166/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:750130/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:1348449/1‑71 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCAGCAAAGGCAATATCGCGa > 1:771795/1‑49 (MQ=255) aCCAAAAGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg > 1:494970/1‑71 (MQ=255) ccAAAGGCGATCTGCTGGAAGGTATTGGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt > 1:1502218/1‑70 (MQ=255) ccAAAGGCGATCTGCTGGAAGGTATTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCcgt > 1:2431251/1‑70 (MQ=255) ccAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCcg < 1:229439/70‑1 (MQ=255) aaaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg > 1:3802691/1‑71 (MQ=255) aaGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCgg > 1:1343771/1‑70 (MQ=255) aGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGc > 1:675117/1‑70 (MQ=255) tgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg > 1:3140412/1‑70 (MQ=255) ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACg < 1:380177/68‑1 (MQ=255) ctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGc < 1:3243876/69‑1 (MQ=255) gAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCa < 1:113803/70‑1 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc < 1:469113/71‑1 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:257430/1‑71 (MQ=255) aGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:1810613/1‑71 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg > 1:495167/1‑71 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg > 1:2745108/1‑71 (MQ=255) ggCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg < 1:376657/71‑1 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:826038/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:779750/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgcc > 1:2657634/1‑71 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:3231192/1‑70 (MQ=255) aTTAGCCGCAAAGTCAATATCGCGACTACCGAAACgc < 1:2643082/37‑1 (MQ=255) aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACgc < 1:3706128/37‑1 (MQ=255) ttAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGc < 1:2781917/59‑1 (MQ=255) tAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGc < 1:2149343/58‑1 (MQ=255) aGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCAt > 1:759775/1‑71 (MQ=255) aGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCAt > 1:2467589/1‑71 (MQ=255) ccGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTc < 1:2533911/71‑1 (MQ=255) cGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCa < 1:579915/55‑1 (MQ=255) aaaGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcg > 1:2181772/1‑59 (MQ=255) aaaGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGt < 1:3540131/70‑1 (MQ=255) aaGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCa < 1:1616443/42‑1 (MQ=255) aaGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGtcc < 1:3047005/71‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGtccatc > 1:1423560/1‑71 (MQ=255) atatCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTc < 1:545822/40‑1 (MQ=255) atatCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCAtt < 1:2527131/59‑1 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGGGGCAAGTCAc > 1:2746571/1‑40 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGt < 1:1782452/37‑1 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGt < 1:735521/37‑1 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGt < 1:2171717/37‑1 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCAc > 1:3801036/1‑40 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCAc > 1:795744/1‑40 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCAc > 1:3240220/1‑40 (MQ=255) atCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTa > 1:2769081/1‑71 (MQ=255) gcgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCt > 1:2862636/1‑71 (MQ=255) gcgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCt > 1:1721481/1‑71 (MQ=255) gcgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCt > 1:2855451/1‑71 (MQ=255) cgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTg < 1:775555/71‑1 (MQ=255) cgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTg < 1:1774885/71‑1 (MQ=255) cgACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTg < 1:1121476/71‑1 (MQ=255) gACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTgg < 1:1581684/71‑1 (MQ=255) | ATCTTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGG > W3110S.gb/3521936‑3522074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |