| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,534,326 | A→T | 7.0% | T222T (ACT→ACA) | kdgT ← | 2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,534,326 | 0 | A | T | 7.0% | 191.4 / 6.1 | 71 | T222T (ACT→ACA) | kdgT | 2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base A (37/29); new base T (2/3); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCGGCGATAATCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGCG > W3110S.gb/3534258‑3534390 | tCGGCGATAATCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGt < 1:3721465/71‑1 (MQ=255) ggCGATAATCAACTGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCa > 1:1098234/1‑67 (MQ=255) ggCGATAATCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCa > 1:973760/1‑67 (MQ=255) gATAATCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTggg < 1:864212/71‑1 (MQ=255) aTCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCaa < 1:2743465/70‑1 (MQ=255) aTCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCaa < 1:3544225/70‑1 (MQ=255) cAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATca > 1:3361759/1‑71 (MQ=255) cAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATca > 1:920884/1‑71 (MQ=255) cAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATca > 1:3567697/1‑71 (MQ=255) aaCTGAATACCGGTCACGATAATTACTTCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATcac < 1:509211/71‑1 (MQ=255) aaCGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATca > 1:1775029/1‑70 (MQ=255) cGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCTGTCTGGGCAATTACAg > 1:527096/1‑71 (MQ=255) aTACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCaa < 1:2098739/71‑1 (MQ=255) tACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGc < 1:2812361/58‑1 (MQ=255) ccGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAAt > 1:798031/1‑70 (MQ=255) ccGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAAt > 1:223646/1‑70 (MQ=255) cGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCa < 1:876061/71‑1 (MQ=255) tCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAAt > 1:1908658/1‑70 (MQ=255) tCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATg < 1:3475217/71‑1 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATcaca > 1:2312415/1‑57 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg < 1:978744/70‑1 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg > 1:2255486/1‑70 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg < 1:3307184/70‑1 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg > 1:1297044/1‑70 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg < 1:447108/70‑1 (MQ=255) aCGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGt < 1:1121085/71‑1 (MQ=255) cGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAg < 1:1730697/42‑1 (MQ=255) cGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATgg < 1:2048374/69‑1 (MQ=255) cGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGt < 1:2330334/70‑1 (MQ=255) gATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTAt > 1:1146722/1‑71 (MQ=255) gATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTAt < 1:501341/71‑1 (MQ=255) tAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTc > 1:2421198/1‑41 (MQ=255) tAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTg > 1:3170016/1‑71 (MQ=255) tAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTg < 1:3700867/71‑1 (MQ=255) aTTACTGCCACACCCAACAGGATCCCCAGCACACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGcc > 1:2518498/1‑71 (MQ=255) aTTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCaa > 1:1001009/1‑46 (MQ=255) aTTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCaa > 1:2312331/1‑46 (MQ=255) aTTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCaa > 1:1724508/1‑46 (MQ=255) aTTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGcc > 1:1326458/1‑71 (MQ=255) tACTGCCACACCCAACAGGATCCCCAGCAAACCTGTCTGGGCAATTACAGTCa > 1:98824/1‑53 (MQ=39) tACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATcac > 1:3734153/1‑48 (MQ=255) tACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGcc > 1:51402/1‑69 (MQ=255) aCTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATcaca > 1:77684/1‑48 (MQ=255) aCTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAg > 1:2705474/1‑71 (MQ=255) cacaCCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGcgcg > 1:341786/1‑70 (MQ=255) cacaCCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGcgcg > 1:3254491/1‑70 (MQ=255) cacaCCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGcgcg > 1:2338798/1‑70 (MQ=255) cacaCCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGcgcg > 1:259338/1‑70 (MQ=255) cacCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGa < 1:1596008/69‑1 (MQ=255) ccAACAGGATCCCCAGCAAACCAGTCTGGGCAATAACAGTCaaa < 1:1909583/44‑1 (MQ=255) aCAGGATCCCCAGCAAACCTGTCTTGGCAATTACAGTCAAATCAATGGTATTGCCCAGTGCGAAGGCAAAg < 1:2831943/71‑1 (MQ=255) aCAGGATCCCCAGCAAACCTGTCTGGGCAATTTCAGTCAAATCAATGGTATTGCCCAGTGCGAAGGCAAAg < 1:1266351/71‑1 (MQ=255) aCAGGATCCCCAGCAAACCTGTCTGGGCAATTACAGTCAAATCAATGGTATTGCCCAGTGCGAAGGCAAAg < 1:2897857/71‑1 (MQ=255) aCAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAg < 1:1416249/71‑1 (MQ=255) aCAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAg < 1:1600056/71‑1 (MQ=255) aCAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAg < 1:2255305/71‑1 (MQ=255) cAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAg > 1:3717485/1‑70 (MQ=255) aTCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATgg < 1:2588661/71‑1 (MQ=255) tCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGa < 1:1007097/71‑1 (MQ=255) cccAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGa < 1:3408536/69‑1 (MQ=255) cAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGt < 1:558425/39‑1 (MQ=255) cAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGt < 1:575286/39‑1 (MQ=255) aGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGc > 1:843244/1‑43 (MQ=255) gCAAACCAGTCTGGGCAATCACAGTCAAATCAATTGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGc > 1:401775/1‑71 (MQ=255) gCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTa > 1:1926418/1‑38 (MQ=255) gCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGc > 1:1978740/1‑71 (MQ=255) gCAAACCAGTCTGGGCAATCACAGTCAAATCAATCGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGc > 1:3225328/1‑71 (MQ=255) cAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGaa > 1:709665/1‑60 (MQ=255) cAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGc > 1:1063724/1‑70 (MQ=255) aaaCCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGCg < 1:3830976/70‑1 (MQ=255) aaCCAGTCTGGGCAATCACAGTCAAATCAATGGTAt > 1:89731/1‑36 (MQ=255) aaCCAGTCTGGGCAATCACAGTCAAATCAATGGTAt > 1:3591682/1‑36 (MQ=255) ccAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAg > 1:521704/1‑65 (MQ=255) | TCGGCGATAATCAACGGAATACCGGTCACGATAATTACTGCCACACCCAACAGGATCCCCAGCAAACCAGTCTGGGCAATCACAGTCAAATCAATGGTATTGCCCAGCGCGAAGGCAAAGAATGGAATCAGCG > W3110S.gb/3534258‑3534390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |