Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,603,753 T→C 7.8% H146R (CAT→CGT) ‡ yigZ ← predicted elongation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,603,7530TC 7.8% 241.5 / 9.1 90H146R (CAT→CGT) ‡yigZpredicted elongation factor
Reads supporting (aligned to +/- strand):  ref base T (47/36);  new base C (2/5);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

TGCCTGATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAGCTGGCGCAGC  >  W3110S.gb/3603683‑3603818
                                                                      |                                                                 
tGCCTGATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACtgat                                                                   <  1:2990620/71‑1 (MQ=255)
tGCCTGATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACtgat                                                                   <  1:3047691/71‑1 (MQ=255)
     gATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGatat                                                              >  1:64184/1‑71 (MQ=255)
     gATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGatat                                                              >  1:15102/1‑71 (MQ=255)
       tAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATAtt                                                             >  1:2679945/1‑70 (MQ=255)
         aTCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTca                                                           >  1:368035/1‑70 (MQ=255)
         aTCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTca                                                           >  1:1769397/1‑70 (MQ=255)
         aTCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGACTATATTca                                                           <  1:2716601/70‑1 (MQ=255)
          tCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTcaca                                                         <  1:457703/71‑1 (MQ=255)
              tGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTc                                                            <  1:1661628/64‑1 (MQ=255)
              tGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTc                                                            <  1:2820230/64‑1 (MQ=255)
                  gATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 >  1:3079943/1‑71 (MQ=255)
                  gATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 >  1:1325884/1‑71 (MQ=255)
                   aTAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 <  1:24374/70‑1 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCACCGGTTCAATGCCGGTTAACTGATGATATTCACAt                                                        >  1:2584583/1‑62 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:2991309/1‑71 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               <  1:1806331/71‑1 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:2226585/1‑71 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               <  1:2349801/71‑1 (MQ=255)
                    tAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:803789/1‑71 (MQ=255)
                      aTTTTGCGGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTcaca                                                         >  1:311464/1‑59 (MQ=255)
                      aTTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTcaca                                                         >  1:3723784/1‑59 (MQ=255)
                       ttttGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTatat                                            >  1:475616/1‑71 (MQ=255)
                         ttGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:3194349/1‑63 (MQ=255)
                         ttGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:2020366/1‑63 (MQ=255)
                         ttGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:2274274/1‑63 (MQ=255)
                         ttGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTc                                          >  1:2793234/1‑71 (MQ=255)
                          tGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTc                                          <  1:1532988/70‑1 (MQ=255)
                              gTCACACTGCCCCAGCAACGCTTGAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTa                                     <  1:2814221/71‑1 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTca                                                           <  1:1310337/46‑1 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTca                                                           <  1:1525955/46‑1 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTc                                                            >  1:2452916/1‑45 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:3284430/1‑55 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:100542/1‑55 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:1295203/1‑55 (MQ=255)
                                 acacTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATg                                  >  1:1135037/1‑71 (MQ=255)
                                      gCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATAtt                                           >  1:2417960/1‑57 (MQ=255)
                                      gCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTc                             >  1:770843/1‑71 (MQ=255)
                                      gCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTc                             >  1:1967248/1‑71 (MQ=255)
                                         ccAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCaaa                                                  >  1:2870369/1‑47 (MQ=255)
                                         ccAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGt                                                <  1:3656447/49‑1 (MQ=255)
                                          cAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGc                         <  1:1773748/71‑1 (MQ=255)
                                           aGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGtata                                             >  1:873085/1‑50 (MQ=255)
                                           aGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGtat                                              >  1:219340/1‑49 (MQ=255)
                                           aGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGc                         <  1:2271241/70‑1 (MQ=255)
                                            gCAACGCTTCAATGCTGGTTAACTGACTATATTCACATTGCAAAGta                                               >  1:3194635/1‑47 (MQ=255)
                                            gCAACGCTTCAATGCCGGTTAACTGATGATATTcaca                                                         <  1:2858829/37‑1 (MQ=255)
                                            gCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGtt                                      <  1:675315/56‑1 (MQ=255)
                                            gCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCt                            >  1:2697444/1‑66 (MQ=255)
                                            gCAACGCTTCAATGCCGGTTAACTGATGATATTAACATTGAAAAGTATATTCGGt                                       >  1:1072178/1‑55 (MQ=255)
                                            gCAACGCTTCAATGCCGGTTAACTGACTATATTCACATTGCAAAGta                                               >  1:775854/1‑47 (MQ=39)
                                             cAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGtt                      >  1:3448001/1‑71 (MQ=255)
                                             cAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGtt                      >  1:1600418/1‑71 (MQ=255)
                                             cAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGtt                      >  1:1605483/1‑71 (MQ=255)
                                             cAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGtt                      >  1:1746814/1‑71 (MQ=255)
                                              aaCGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGt                       >  1:1836608/1‑69 (MQ=255)
                                              aaCGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTg                     <  1:1065178/71‑1 (MQ=255)
                                               aCGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTgg                    <  1:898600/71‑1 (MQ=255)
                                               aCGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTgg                    <  1:1634313/71‑1 (MQ=255)
                                               aCGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTgg                    <  1:1154287/71‑1 (MQ=255)
                                                cGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               <  1:1754593/43‑1 (MQ=255)
                                                cGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               <  1:1750200/43‑1 (MQ=255)
                                                  cTTCAATGCCTGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTg                     <  1:320199/67‑1 (MQ=255)
                                                   ttCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGtt                                      <  1:511995/49‑1 (MQ=255)
                                                   ttCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGt                  <  1:1244676/69‑1 (MQ=255)
                                                     cAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGt               >  1:1847197/1‑70 (MQ=255)
                                                      aaTGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 <  1:2492459/35‑1 (MQ=255)
                                                      aaTGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 <  1:1219577/35‑1 (MQ=255)
                                                      aaTGCCGGTTAACTGATGATATTCACATTGCAAAg                                                 <  1:1202487/35‑1 (MQ=255)
                                                      aaTGCCGGTTAACTGACTATATTCACATTGCAAAGTATATTc                                          <  1:2514434/42‑1 (MQ=38)
                                                      aaTGCCGGTTAACTGACTATATTCACATTGCAAAGTATATTc                                          <  1:2930813/42‑1 (MQ=38)
                                                      aaTGCCGGTTAACTGACTATATTCACATTGCAAAGTATATTc                                          <  1:1408692/42‑1 (MQ=38)
                                                      aaTGCCGGTTAACTGACTATATTCACATTGCAAAGTATATTc                                          <  1:1395665/42‑1 (MQ=38)
                                                       aTGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGtt              <  1:2452662/69‑1 (MQ=255)
                                                       aTGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGtt              <  1:1615657/69‑1 (MQ=255)
                                                        tGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:3498253/1‑35 (MQ=255)
                                                        tGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:2728256/1‑35 (MQ=255)
                                                        tGCCGGTTAACTGATGATATTCACATTGCAAAGta                                               >  1:786207/1‑35 (MQ=255)
                                                         gCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGtt                                      <  1:1196047/43‑1 (MQ=255)
                                                         gCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAg            >  1:2534465/1‑69 (MQ=255)
                                                         gCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAg            >  1:2239282/1‑69 (MQ=255)
                                                         gCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAg            >  1:3198518/1‑69 (MQ=255)
                                                         gCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAg            >  1:1748448/1‑69 (MQ=255)
                                                          ccGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTaa                                    >  1:1024454/1‑44 (MQ=255)
                                                          ccGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAGCTg         >  1:2155727/1‑71 (MQ=255)
                                                              ttAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTg                     <  1:2810782/55‑1 (MQ=255)
                                                               tAACTGATGATATTCACATTGCAAAGTATATTCGGTTa                                     <  1:2207457/38‑1 (MQ=255)
                                                               tAACTGATGATATTCACATTGCAAAGTATATTCGGTTa                                     <  1:1129666/38‑1 (MQ=255)
                                                               tAACTGATGATATTCACATTGCAAAGTATATTCGGTTa                                     <  1:2994146/38‑1 (MQ=255)
                                                               tAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGt                  <  1:708848/57‑1 (MQ=255)
                                                                aaCTGATGATATTCACATTGAAAAGTATATTCGGTTAATGGCGTCtt                           >  1:1323123/1‑47 (MQ=255)
                                                                 aCTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAGCTGGCGCAgc  <  1:3651115/71‑1 (MQ=255)
                                                                     atgatgTTCACATTGCAAAGTATATTCGGTTAATGGCGt                              >  1:2712684/1‑39 (MQ=255)
                                                                      |                                                                 
TGCCTGATAATCACTGTTGATAATTTTGCCGTCACACTGCCCCAGCAACGCTTCAATGCCGGTTAACTGATGATATTCACATTGCAAAGTATATTCGGTTAATGGCGTCTTGCGTTGGGTCGTTAGCTGGCGCAGC  >  W3110S.gb/3603683‑3603818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: