| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,657,715 | A→G | 8.8% | G122G (GGT→GGC) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,657,715 | 0 | A | G | 8.8% | 147.9 / 5.3 | 57 | G122G (GGT→GGC) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base A (25/27); new base G (2/3); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TGTAGGAGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCGGACGGCGCGG > W3110S.gb/3657645‑3657780 | tGTAGGAGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATa > 1:2846856/1‑71 (MQ=255) ggAGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCa < 1:824980/71‑1 (MQ=255) ggAGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCa < 1:2148292/71‑1 (MQ=255) aGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATc < 1:272206/71‑1 (MQ=255) aGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATc < 1:1857327/71‑1 (MQ=255) aaGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAgg < 1:2608280/70‑1 (MQ=255) gCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTCACCAGAGCGATGCCCATCAGGAt > 1:1583749/1‑70 (MQ=255) gCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTCACCAGAGCGATGCCCATCAGGAt > 1:3258201/1‑70 (MQ=255) aaCAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTCACCAGAGCGATGCCCAt < 1:1369515/59‑1 (MQ=255) aaCAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTCACCAGAGCGATGCCCAt < 1:171220/59‑1 (MQ=255) aaCAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTCACCAGAGCGATGCCCAt < 1:2404098/59‑1 (MQ=255) aCAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCAc > 1:1032550/1‑71 (MQ=255) cAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACg > 1:822389/1‑71 (MQ=255) gCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGt > 1:601794/1‑70 (MQ=255) cAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:472810/71‑1 (MQ=255) cAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:588600/71‑1 (MQ=255) cAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:302761/71‑1 (MQ=255) aaaaaGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:1928293/70‑1 (MQ=255) aaaaaGCCGTTGTGCATGAAGAAGACGCCGACGCTTACCATCGCGATACCCATCAGGATCACCCa > 1:2044673/1‑65 (MQ=255) aaaaaGCCGTTGTGCATGAAGAAGACGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCa > 1:460032/1‑65 (MQ=255) aaaaGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAg < 1:3849356/70‑1 (MQ=255) aaaGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGGGCGATACCTATCAGGATCACCCACGTAAGAt < 1:192721/71‑1 (MQ=255) aaaGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGAt < 1:820743/71‑1 (MQ=255) aaaGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGAt < 1:821356/71‑1 (MQ=255) aaaGCCGTTGCGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGAt < 1:3817639/71‑1 (MQ=255) ccGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:830130/64‑1 (MQ=255) gTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTg > 1:1405113/1‑67 (MQ=255) gTTGTGCATGAAGAAGATACCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTg > 1:517022/1‑67 (MQ=255) ttGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGa < 1:3663230/63‑1 (MQ=255) ttGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGa < 1:3657795/63‑1 (MQ=255) ttGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGtctc < 1:3661338/71‑1 (MQ=255) gtgCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTgg > 1:2918484/1‑65 (MQ=255) gtgCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTgg > 1:3646389/1‑65 (MQ=255) gtgCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCa > 1:480651/1‑71 (MQ=255) cATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATAACCCACGTAAGATTGGt > 1:3495130/1‑63 (MQ=255) aTGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCAcc < 1:1813971/48‑1 (MQ=255) gaagaaGATGCCGACGCTTACCAGCGCGATACCCATCAg < 1:1028186/39‑1 (MQ=255) gaagaaGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGt > 1:2266159/1‑60 (MQ=255) agaTGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATg > 1:767575/1‑71 (MQ=255) gaTGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATgg > 1:2916349/1‑71 (MQ=255) aTGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGt < 1:2984825/44‑1 (MQ=255) tGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGt > 1:3699530/1‑70 (MQ=255) tGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGt > 1:3851919/1‑70 (MQ=255) tGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGt > 1:1895402/1‑70 (MQ=255) tGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGt > 1:2588458/1‑70 (MQ=255) tGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGt > 1:1360564/1‑70 (MQ=255) gCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTa > 1:2039836/1‑70 (MQ=255) gACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCAt > 1:3070417/1‑63 (MQ=255) cGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACa > 1:3343828/1‑69 (MQ=255) cGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACa > 1:118143/1‑69 (MQ=255) gCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:3371757/38‑1 (MQ=255) gCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:1833349/38‑1 (MQ=255) gCTTACCAGCGCGATACCCATCAGGATCACCCACGTaa < 1:181900/38‑1 (MQ=255) gCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCg > 1:3785811/1‑71 (MQ=255) aCCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCGGAc < 1:2163520/70‑1 (MQ=255) gcgcGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCGGACGgcgc < 1:2829513/71‑1 (MQ=255) gcgATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCGGACGGCGCgg > 1:3469320/1‑71 (MQ=255) tACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCAt < 1:2270461/46‑1 (MQ=255) | TGTAGGAGTTAAGCCGGAACAGCAAAAAGCCGTTGTGCATGAAGAAGATGCCGACGCTTACCAGCGCGATACCCATCAGGATCACCCACGTAAGATTGGTCTCCACGCGGTTCATGGTAAACAGCGGACGGCGCGG > W3110S.gb/3657645‑3657780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |