| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,674,367 | T→C | 6.4% | E546E (GAA→GAG) | rep ← | DNA helicase and single‑stranded DNA‑dependent ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,674,367 | 0 | T | C | 6.4% | 166.5 / 2.5 | 62 | E546E (GAA→GAG) | rep | DNA helicase and single‑stranded DNA‑dependent ATPase |
| Reads supporting (aligned to +/- strand): ref base T (32/26); new base C (2/2); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GTAGACATAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCGGCT > W3110S.gb/3674298‑3674437 | gTAGACATAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCGCCTGATCCAGCTcttctt > 1:899914/1‑71 (MQ=255) tAGACATAAGGAATCTCCAGCCCTTTCGACGGGTGGAGAGTCATCAGTTGCACCTGATCCAGCTcttctt < 1:667397/70‑1 (MQ=255) tAGACATAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCttcttc < 1:418145/71‑1 (MQ=255) gACATAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTcttctt < 1:2630631/68‑1 (MQ=255) tAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTcttctt < 1:2967378/64‑1 (MQ=255) aGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTcac > 1:1922286/1‑71 (MQ=255) aGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTcac < 1:1800637/71‑1 (MQ=255) aGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTca < 1:3797915/70‑1 (MQ=255) aaCTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTcacc > 1:2605961/1‑68 (MQ=255) aCTCCAGCCCTTTCGACGCGTGCAGAGTCATCAGTTGCACCTGATCCAGCTCTTCCTCGCTCTCGCcgcg > 1:3027795/1‑70 (MQ=255) aCTCCAGCCCTTTCGACGCGTGCAGAGTCATCAGTTGCACCTGATCCAGCTCTTCCTCGCTCTCGCCgcgc > 1:399752/1‑71 (MQ=255) tCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTc > 1:1471771/1‑71 (MQ=255) cAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCca > 1:1817308/1‑71 (MQ=255) cTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCActct < 1:2785385/54‑1 (MQ=255) cTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATg > 1:3521413/1‑71 (MQ=255) tttCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCttcttc < 1:2303594/48‑1 (MQ=255) tttAGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCttcttc < 1:2667642/48‑1 (MQ=255) tCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTcg > 1:1575973/1‑71 (MQ=255) cgcgTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCa < 1:2622032/71‑1 (MQ=255) tGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGt < 1:989254/71‑1 (MQ=255) gagaGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTa > 1:134183/1‑70 (MQ=255) agagTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTaa < 1:39770/70‑1 (MQ=255) agTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgc < 1:2122371/71‑1 (MQ=255) tcatcaGTTTCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTaa < 1:260281/66‑1 (MQ=255) tcatcaGTTGCACCTGATGCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTaa < 1:2753726/66‑1 (MQ=255) tcatcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTaa < 1:2702120/66‑1 (MQ=255) tcatcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc < 1:1897563/71‑1 (MQ=255) tcatcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc < 1:3554513/71‑1 (MQ=255) tcatcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc < 1:3559007/71‑1 (MQ=255) atcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGt < 1:1955332/71‑1 (MQ=255) tcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCcatcat < 1:2565874/49‑1 (MQ=255) tcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCcatcat < 1:473499/49‑1 (MQ=255) tcaGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGt < 1:1817755/61‑1 (MQ=255) tcaGTTGAACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCcatcat < 1:3137148/49‑1 (MQ=255) caGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTc > 1:1990359/1‑42 (MQ=255) caGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTc > 1:2085490/1‑42 (MQ=255) caGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTca > 1:3295639/1‑71 (MQ=255) aGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTaa > 1:52562/1‑61 (MQ=255) aGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcac < 1:2881074/71‑1 (MQ=255) aGTTGCACCTGATCCAGCTCTTCCTCGCTCTCGCCGCGCTCCATCATGTCGCGCAAAGTAAAg < 1:1487106/63‑1 (MQ=255) aGTTGCACCTGATCCAGCTCTTCCTCGCTCTCGCCGCGCTCCATCATGTCGCGCAAAGTAAAg < 1:3711931/63‑1 (MQ=255) tGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc > 1:157270/1‑63 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcaccac > 1:919485/1‑70 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCaccacc > 1:3211608/1‑71 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCaccacc > 1:1027291/1‑71 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCaccacc > 1:263531/1‑71 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCaccacc > 1:633384/1‑71 (MQ=255) gCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCaccacc > 1:3148154/1‑71 (MQ=255) cTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCa < 1:169046/47‑1 (MQ=255) cTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCa < 1:1925022/47‑1 (MQ=255) cTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcacc < 1:3137285/64‑1 (MQ=255) cTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcacc < 1:224415/64‑1 (MQ=255) tGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc > 1:509264/1‑57 (MQ=255) tGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgcgc > 1:2122882/1‑57 (MQ=255) tGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:3183671/1‑71 (MQ=255) gATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAgc > 1:3658275/1‑54 (MQ=255) gATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:466938/1‑70 (MQ=255) gATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:1818641/1‑70 (MQ=255) gATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:1971821/1‑70 (MQ=255) aTCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcaccac > 1:697749/1‑63 (MQ=255) aTCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcaccac > 1:72575/1‑63 (MQ=255) aTCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTggg > 1:1592559/1‑68 (MQ=255) aTCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:3439271/1‑69 (MQ=255) aTCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGt > 1:725969/1‑69 (MQ=255) ccAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGc > 1:1501669/1‑71 (MQ=255) cAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcacc < 1:943126/58‑1 (MQ=255) gCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcaccac > 1:910730/1‑58 (MQ=255) ctctTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGt < 1:2385861/69‑1 (MQ=255) ctctTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGt < 1:3843728/69‑1 (MQ=255) cttcttCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTc < 1:153751/68‑1 (MQ=255) ttcttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCg > 1:2797682/1‑71 (MQ=255) ttcttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCg > 1:3172833/1‑71 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcac > 1:2305500/1‑49 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcac > 1:2318750/1‑49 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcac > 1:2606226/1‑49 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTcac > 1:3463101/1‑49 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTggg > 1:1193381/1‑57 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTggg > 1:1263266/1‑57 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTggg > 1:2920359/1‑57 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTgg > 1:1552232/1‑56 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCgg > 1:2065190/1‑70 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCGGc > 1:1233874/1‑71 (MQ=255) cttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCGGc > 1:1001081/1‑71 (MQ=255) ttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTc < 1:2808134/46‑1 (MQ=255) ttcACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCGGCt > 1:2569411/1‑71 (MQ=255) ttcACTCTCACCACGCTCCATCATGTCGCGCAAAGT‑AAGCGCGTc < 1:1468810/45‑1 (MQ=255) | GTAGACATAAGGAAACTCCAGCCCTTTCGACGCGTGGAGAGTCATCAGTTGCACCTGATCCAGCTCTTCTTCACTCTCACCACGCTCCATCATGTCGCGCAAAGTAAAGCGCGTCACCACCTGGGTGAGCGTCATCGGCT > W3110S.gb/3674298‑3674437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |