| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,716,825 | C→A | 9.1% | intergenic (+12/‑1) | atpH → / → atpA | F1 sector of membrane‑bound ATP synthase, delta subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,716,825 | 0 | C | A | 9.1% | 199.9 / 4.1 | 65 | intergenic (+12/‑1) | atpH/atpA | F1 sector of membrane‑bound ATP synthase, delta subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/35); new base A (4/2); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GATGGCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTGTGAGTG > W3110S.gb/3716760‑3716895 | gATGGCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGc < 1:1936508/70‑1 (MQ=255)gATGGCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGc > 1:1122272/1‑70 (MQ=255)gATGGCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGc > 1:7151/1‑70 (MQ=255) gCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTg < 1:3756242/71‑1 (MQ=255) gCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTg < 1:3786164/71‑1 (MQ=255) cAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGa < 1:624223/71‑1 (MQ=255) cAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGa < 1:3488867/71‑1 (MQ=255) cGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGc > 1:1782795/1‑58 (MQ=255) cgcgGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCAc > 1:1286178/1‑71 (MQ=255) cgcgGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCAc > 1:750283/1‑71 (MQ=255) cgcgGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCAc > 1:2203786/1‑71 (MQ=255) gcgGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGTGCATGCAACTGAATTCCAcc < 1:3433536/71‑1 (MQ=255) tcgtcTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGaaa < 1:2871582/71‑1 (MQ=255) cgtcTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt > 1:2532122/1‑71 (MQ=255) gtcTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt < 1:1698061/70‑1 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGc > 1:1674101/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGc > 1:3431046/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGc > 1:2314370/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGc > 1:2453651/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGc > 1:1796068/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGc > 1:2030320/1‑71 (MQ=255) ttGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGc > 1:2025794/1‑71 (MQ=255) ttGAGCGCCTT‑CAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGc > 1:771856/1‑70 (MQ=255) gAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCg > 1:1760173/1‑70 (MQ=255) atcgcCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGaa < 1:2159385/69‑1 (MQ=255) cgcCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACt < 1:2348151/71‑1 (MQ=255) ccTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGa < 1:1048830/71‑1 (MQ=255) tGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCg < 1:2380276/62‑1 (MQ=255) tGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCg < 1:2393069/62‑1 (MQ=255) cGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGa < 1:2333306/38‑1 (MQ=255) gTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:3826833/71‑1 (MQ=255) gTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:3102805/71‑1 (MQ=255) cTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTc > 1:1225705/1‑39 (MQ=255) cTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTc > 1:1936633/1‑39 (MQ=255) gCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGaa > 1:3005070/1‑52 (MQ=255) aGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCt < 1:2231111/71‑1 (MQ=255) gTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:2218024/63‑1 (MQ=255) gTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTc < 1:538378/71‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt < 1:1073146/41‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt < 1:1308953/41‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt < 1:58672/41‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAAt < 1:2000966/41‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcgc < 1:2574275/63‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcgc < 1:1631962/63‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:3812092/62‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:2105408/62‑1 (MQ=255) tCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAgcg < 1:3650327/62‑1 (MQ=255) ttAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGt > 1:683944/1‑71 (MQ=255) ttAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAACGAACTGATCAAGCAGCGCATTGCTCAGt > 1:320740/1‑71 (MQ=255) aaGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGaa > 1:2861509/1‑44 (MQ=255) aaGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGaa > 1:2048493/1‑44 (MQ=255) aaGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAg < 1:1350014/68‑1 (MQ=255) aaGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGtt > 1:289446/1‑70 (MQ=255) aaGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGtt > 1:2937775/1‑70 (MQ=255) aaGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGt < 1:665129/69‑1 (MQ=255) aaGGGGACTGGAGAATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGt < 1:2527386/69‑1 (MQ=255) aGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGa > 1:1172835/1‑47 (MQ=255) aGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTc > 1:2271053/1‑65 (MQ=255) aGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTc < 1:2294440/70‑1 (MQ=255) aGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTc < 1:1485970/70‑1 (MQ=255) aGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTc < 1:3014294/70‑1 (MQ=255) ggggACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCaa < 1:3360352/71‑1 (MQ=255) ggggACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCaa < 1:70316/71‑1 (MQ=255) gACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAAt > 1:2646188/1‑69 (MQ=255) cTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAAtgttg < 1:2377696/71‑1 (MQ=255) aGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTgtgag > 1:234490/1‑71 (MQ=255) cATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTgtgagtg < 1:481781/71‑1 (MQ=255) cATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTgtgagtg < 1:374873/71‑1 (MQ=255) cATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTgtgagtg < 1:1919609/71‑1 (MQ=255) | GATGGCAGCGTACGCGGTCGTCTTGAGCGCCTTGCAGACGTCTTGCAGTCTTAAGGGGACTGGAGCATGCAACTGAATTCCACCGAAATCAGCGAACTGATCAAGCAGCGCATTGCTCAGTTCAATGTTGTGAGTG > W3110S.gb/3716760‑3716895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |