| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,800,285 | C→T | 5.7% | intergenic (‑83/‑139) | yicS ← / → nlpA | hypothetical protein/cytoplasmic membrane lipoprotein‑28 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,800,285 | 0 | C | T | 5.7% | 269.6 / 3.7 | 87 | intergenic (‑83/‑139) | yicS/nlpA | hypothetical protein/cytoplasmic membrane lipoprotein‑28 |
| Reads supporting (aligned to +/- strand): ref base C (43/39); new base T (3/2); total (46/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
TACAAATGTGTATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCTTATCGATAG > W3110S.gb/3800216‑3800354 | tACAAATGTGTATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAAcgc < 1:347889/70‑1 (MQ=255) aTGTGTATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGc > 1:316110/1‑71 (MQ=255) aTGTGTATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGc > 1:409363/1‑71 (MQ=255) tgtATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGt < 1:2870075/70‑1 (MQ=255) tCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCg < 1:522366/65‑1 (MQ=255) tCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGt > 1:1049503/1‑69 (MQ=255) cGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAg > 1:3105488/1‑71 (MQ=255) gAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAg > 1:2868012/1‑70 (MQ=255) gAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGt < 1:1977201/71‑1 (MQ=255) gAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGt < 1:3850805/71‑1 (MQ=255) aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAg > 1:1218865/1‑69 (MQ=255) aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGt > 1:2778290/1‑70 (MQ=255) aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGt > 1:1200446/1‑70 (MQ=255) aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTa < 1:725962/71‑1 (MQ=255) aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACAAAACGCGCTTGCGTGGTCAGt > 1:1429298/1‑70 (MQ=255) aTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaa < 1:2353945/71‑1 (MQ=255) tGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAAcgcg < 1:3508112/54‑1 (MQ=255) tGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGt > 1:2589740/1‑68 (MQ=255) tGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaag < 1:1621941/71‑1 (MQ=255) cATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaag < 1:665239/66‑1 (MQ=255) cATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTg < 1:118867/71‑1 (MQ=255) tAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACgcgc < 1:598022/48‑1 (MQ=255) tAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACgcgc < 1:10324/48‑1 (MQ=255) tAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaag < 1:2290572/64‑1 (MQ=255) tAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGc > 1:1297763/1‑70 (MQ=255) tAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGc > 1:3357174/1‑70 (MQ=255) aGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGt > 1:2404199/1‑55 (MQ=255) aGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCa < 1:3143648/70‑1 (MQ=255) aGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCa < 1:1557789/70‑1 (MQ=255) gtgtAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaa < 1:842328/60‑1 (MQ=255) tgtAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTTGTCAGTaa < 1:2146663/59‑1 (MQ=255) tAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTg < 1:1687767/49‑1 (MQ=255) tAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTg < 1:1386442/49‑1 (MQ=255) tAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTg < 1:2481608/49‑1 (MQ=255) aGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACtt > 1:239928/1‑70 (MQ=255) gTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTta < 1:2046156/71‑1 (MQ=255) gTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTta > 1:1246369/1‑71 (MQ=255) tGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTatat > 1:2962208/1‑71 (MQ=255) gCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTatat < 1:2540975/70‑1 (MQ=255) gCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATAtt > 1:962416/1‑71 (MQ=255) cTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTta < 1:552566/66‑1 (MQ=255) tttCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaaga < 1:3819484/51‑1 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCg < 1:2788243/37‑1 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaagaa > 1:2145469/1‑50 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTaaga > 1:2465910/1‑49 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:2979543/1‑65 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:2020314/1‑65 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTtat > 1:3041539/1‑64 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTcc < 1:3823316/69‑1 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCAc > 1:211293/1‑71 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCAc > 1:2986797/1‑71 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCAc > 1:3171028/1‑71 (MQ=255) tCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCAc > 1:1005199/1‑71 (MQ=255) aTCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACtt > 1:235164/1‑71 (MQ=255) aTCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACtt < 1:3516929/71‑1 (MQ=255) aTCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACt > 1:1164403/1‑70 (MQ=255) cGCTCTCATCATGCTGTACGAAACGCGCGTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACttt > 1:2516935/1‑70 (MQ=255) ctcATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTc > 1:2859766/1‑71 (MQ=255) ctcATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTc > 1:2450010/1‑71 (MQ=255) tcatcaTGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACttt < 1:3501183/65‑1 (MQ=255) catcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt > 1:851024/1‑71 (MQ=255) atcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt < 1:2811989/70‑1 (MQ=255) tcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt < 1:3294931/69‑1 (MQ=255) tcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt < 1:580436/69‑1 (MQ=255) tcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt < 1:1193403/69‑1 (MQ=255) tcatGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCt < 1:794216/69‑1 (MQ=255) catGCTGTACGAAAGGTGTTTGCGTGGTCAGTaagaa > 1:1115128/1‑37 (MQ=25) catGCTGTACGAAAGGTGTTTGCGTGGTCAGTaagaa > 1:3337020/1‑37 (MQ=25) catGCTGTACGAAAGGTGTTTGCGTGGTCAGTaagaa > 1:2860657/1‑37 (MQ=25) atGCTGTACGAAACGCGCTTGCGTGGTCAGTaagaa > 1:2349598/1‑36 (MQ=255) atGCTGTACGAAACGCGCTTGCGTGGTCAGTaagaa > 1:1607563/1‑36 (MQ=255) atGCTGTACGAAACGCGCTTGCGTGGTCAGTaagaa > 1:2888612/1‑36 (MQ=255) atGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCtttt < 1:16189/70‑1 (MQ=255) atGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCtttt > 1:1663205/1‑70 (MQ=255) cTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTatat < 1:2970633/49‑1 (MQ=255) cTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTAt < 1:1290852/70‑1 (MQ=255) gTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCt > 1:3440533/1‑71 (MQ=255) tACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCtt < 1:2051529/71‑1 (MQ=255) tACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCtt < 1:3353033/71‑1 (MQ=255) tACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCt > 1:318418/1‑70 (MQ=255) aCGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCtt < 1:3009999/70‑1 (MQ=255) aCGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCtt < 1:3110260/70‑1 (MQ=255) aCGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:2257174/40‑1 (MQ=255) aCGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:569279/1‑44 (MQ=255) cGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCa > 1:1026098/1‑48 (MQ=255) cGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTa > 1:613871/1‑54 (MQ=255) aaCGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTta < 1:478146/38‑1 (MQ=255) aaCGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTta < 1:1235939/38‑1 (MQ=255) aaCGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCa > 1:1913572/1‑45 (MQ=255) aaCGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCTTATc < 1:1686998/69‑1 (MQ=255) aCGCGCTTGCGTGGTCAGTAAGAAGTGACAGACTTTATATTCCACTTTTATTCCTTTTTATTCTTATcag > 1:3699883/1‑68 (MQ=255) gcgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCTTATCGATAg < 1:475186/71‑1 (MQ=255) cgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:1347972/1‑36 (MQ=255) cgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:1724980/1‑36 (MQ=255) cgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:2808182/1‑36 (MQ=255) cgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:2566049/1‑36 (MQ=255) cgcTTGCGTGGTCAGTAAGAAGTGCCAGACTTtata > 1:3326896/1‑36 (MQ=255) | TACAAATGTGTATCGAATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTTTATATTCCACTTTTATTCCTTTTTATTCTTATCGATAG > W3110S.gb/3800216‑3800354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |