BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,804,817	C→T	6.5%	I110I (ATC→ATT)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,804,817	0	C	T	6.5%	425.5 / 12.1	138	I110I (ATC→ATT)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (82/47); new base T (2/7); total (84/54)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 140 total aligned reads displayed.

CTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTGCGCATTGG  >  W3110S.gb/3804749‑3804887

cTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                      <  1:763437/71‑1 (MQ=255)

   ccATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATg                                                                    >  1:2224586/1‑70 (MQ=255)

   ccATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATg                                                                    >  1:3064972/1‑70 (MQ=255)

     aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcag                                                                 >  1:1833858/1‑71 (MQ=255)

     aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcag                                                                 >  1:2359110/1‑71 (MQ=255)

       tCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCt                                                                       >  1:3719361/1‑63 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGaaa                                                              >  1:3596523/1‑71 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:1084039/1‑71 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:1656742/1‑71 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:2067882/1‑71 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:2396968/1‑71 (MQ=255)

        cGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:3071221/1‑71 (MQ=255)

         gggATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAt                                                             <  1:1823604/71‑1 (MQ=255)

             tCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttact                                                         >  1:1185445/1‑71 (MQ=255)

              cGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttac                                                          >  1:3637723/1‑69 (MQ=255)

                  tgtgTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTacac                                                    >  1:1873778/1‑71 (MQ=255)

                  tgtgTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTacac                                                    >  1:48042/1‑71 (MQ=255)

                  tgtgTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGATTTATgcagc                                                                >  1:400059/1‑59 (MQ=255)

                      tACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgca                                                                  >  1:2518840/1‑53 (MQ=255)

                      tACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgca                                                                  >  1:1096952/1‑53 (MQ=255)

                           gCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtac                                           >  1:1176894/1‑71 (MQ=255)

                           gCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtac                                           <  1:650151/71‑1 (MQ=255)

                           gCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtac                                           >  1:3538276/1‑71 (MQ=255)

                           gCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActt                                             <  1:3850815/69‑1 (MQ=255)

                           gCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTActt                                             <  1:1760471/69‑1 (MQ=255)

                             cTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         <  1:2934468/71‑1 (MQ=255)

                              tataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         >  1:2840534/1‑70 (MQ=255)

                              tataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         >  1:2283235/1‑70 (MQ=255)

                              tataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         >  1:963489/1‑70 (MQ=255)

                              tataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         >  1:1770008/1‑70 (MQ=255)

                              tataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacct                                         >  1:151222/1‑70 (MQ=255)

                               ataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                       >  1:3415459/1‑55 (MQ=255)

                               ataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacctta                                       >  1:366383/1‑71 (MQ=255)

                               ataGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtacctta                                       >  1:1161957/1‑71 (MQ=255)

                                taGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTa                                                                      <  1:1478601/39‑1 (MQ=255)

                                taGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATgcagca                                                               <  1:358802/46‑1 (MQ=255)

                                taGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactt                                                        >  1:1106296/1‑53 (MQ=255)

                                taGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACAcc                                                   >  1:1343964/1‑58 (MQ=255)

                                 aGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACt                                     >  1:3586291/1‑71 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACCCCCTa                                                >  1:1837825/1‑59 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTAc                                               >  1:1454859/1‑60 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACtt                                    >  1:2465710/1‑71 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACtt                                    <  1:3383599/71‑1 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACtt                                    <  1:2236532/71‑1 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACt                                     >  1:541202/1‑70 (MQ=255)

                                  gCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACt                                     >  1:2996658/1‑70 (MQ=255)

                                    aCGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCaa                                                              >  1:1786834/1‑43 (MQ=255)

                                    aCGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  <  1:2446726/71‑1 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  >  1:3314270/1‑70 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  >  1:3368545/1‑70 (MQ=255)

                                     cGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  >  1:2681544/1‑70 (MQ=255)

                                         aGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTtata                                >  1:692324/1‑68 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAt                                                             <  1:3314170/38‑1 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAAt                                                             <  1:1745987/38‑1 (MQ=255)

                                          gATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACcgt                            >  1:3714408/1‑71 (MQ=255)

                                           aTCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                       <  1:2538141/43‑1 (MQ=255)

                                           aTCTCAGTATGAACGGCAAAATGATCTATGCAGCAAttactta                                                       <  1:1304222/43‑1 (MQ=255)

                                           aTCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACcgtc                           >  1:2279824/1‑71 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAAttac                                                          >  1:3145950/1‑38 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                      >  1:1084351/1‑58 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                      >  1:600278/1‑58 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTtat                                 >  1:1230166/1‑63 (MQ=255)

                                             ctcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACcgtcgt                         >  1:1896710/1‑71 (MQ=255)

                                              tcAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActta                                            <  1:1204119/51‑1 (MQ=255)

                                                aGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:101789/69‑1 (MQ=255)

                                                aGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:264013/69‑1 (MQ=255)

                                                aGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:1403288/69‑1 (MQ=255)

                                                aGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                      <  1:1238926/71‑1 (MQ=255)

                                                aGTATGAACGGCAAAATGATCTATGCAGCAATTACTTAAACCCTACTTACCTTACTTTATACCGTCGTCaa                      <  1:1416690/71‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTActtaccttac                                      <  1:1550873/53‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  <  1:2944864/57‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACcgtcgt                         <  1:2027623/66‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACcgtcgt                         <  1:1159708/66‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:3717148/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:2544892/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCgtcgtc                        <  1:3813629/67‑1 (MQ=255)

                                                  tATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACCTTATACcgtcgt                         <  1:77061/66‑1 (MQ=255)

                                                   aTGAACGGCAAAATGATCTATGCAGCAATTACTTACAACCTActtaccttac                                      >  1:417891/1‑52 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCaa                      >  1:2184002/1‑67 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAatat                   <  1:3778497/70‑1 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAatat                   <  1:3218239/70‑1 (MQ=255)

                                                    tGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATc                  >  1:318610/1‑71 (MQ=255)

                                                     gAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCATCGTCa                       <  1:2957680/65‑1 (MQ=255)

                                                     gAACGGAAAAATGATTTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCATCGTCa                       <  1:689017/65‑1 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  >  1:2356868/1‑53 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTta                                  >  1:1410118/1‑53 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATcc                 >  1:643781/1‑70 (MQ=255)

                                                      aaCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACAGTCGTCAATATccc                >  1:755976/1‑71 (MQ=255)

                                                       aCGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCa                       >  1:1648583/1‑63 (MQ=255)

                                                         ggCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata                    >  1:2776034/1‑64 (MQ=255)

                                                         ggCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAata                    >  1:913335/1‑64 (MQ=255)

                                                         ggCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTa             <  1:2614584/71‑1 (MQ=255)

                                                              aaTGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTgcg        >  1:1455004/1‑71 (MQ=255)

                                                              aaTGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTgcg        <  1:3551867/71‑1 (MQ=255)

                                                              aaTGATCTATGCAACAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCt               <  1:1351252/64‑1 (MQ=255)

                                                               aTGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTgcg        >  1:2851687/1‑70 (MQ=255)

                                                               aTGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTgcg        >  1:233678/1‑70 (MQ=255)

                                                               aTGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACAgcg        >  1:1861982/1‑70 (MQ=255)

                                                                tGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTGCGCa      <  1:2962112/71‑1 (MQ=255)

                                                                   tCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCa                       >  1:2686754/1‑51 (MQ=255)

CTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGATCTATGCAGCAATTACTTACACCCTACTTACCTTACTTTATACCGTCGTCAATATCCCTTACTGCGCATTGG  >  W3110S.gb/3804749‑3804887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`