BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,805,429	C→T	8.6%	P314P (CCC→CCT)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,805,429	0	C	T	8.6%	369.3 / 16.9	128	P314P (CCC→CCT)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (57/58); new base T (8/3); total (65/63)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 130 total aligned reads displayed.

TAAAGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACC  >  W3110S.gb/3805360‑3805497

tAAAGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCa                                                                     >  1:2702948/1‑71 (MQ=255)

  aaGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCAt                                                                    >  1:2370876/1‑70 (MQ=255)

  aaGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATg                                                                   <  1:2763756/71‑1 (MQ=255)

         tATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAg                                                                <  1:1050740/67‑1 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCa                                                            >  1:3416536/1‑69 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCa                                                            >  1:3643365/1‑69 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           <  1:3579228/70‑1 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           <  1:2538006/70‑1 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           <  1:3042718/70‑1 (MQ=255)

           tcttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           >  1:459815/1‑70 (MQ=255)

             ttctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCAt                                                        >  1:3473726/1‑71 (MQ=255)

              tctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCAt                                                        >  1:1747307/1‑70 (MQ=255)

               ctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCAATGCAGGACAGCATc                                                       <  1:1792184/70‑1 (MQ=255)

               ctGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCAATGCAGGACAGCATc                                                       <  1:1796603/70‑1 (MQ=255)

               ctGGTGGACGAACGACCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCa                                                      >  1:3263972/1‑71 (MQ=255)

                  gtggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACt                                                    <  1:429693/70‑1 (MQ=255)

                   tggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTAt                                                  <  1:616499/71‑1 (MQ=255)

                    ggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATg                                                 >  1:3043960/1‑71 (MQ=255)

                     gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATg                                                                   <  1:1103988/52‑1 (MQ=255)

                     gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                <  1:1976779/71‑1 (MQ=255)

                     gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                >  1:1001013/1‑71 (MQ=255)

                     gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                >  1:238193/1‑71 (MQ=255)

                        gAACGCCCTGCTGGCAGTTATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           <  1:389656/57‑1 (MQ=255)

                          aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCa                                                         >  1:1263689/1‑57 (MQ=255)

                          aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                           <  1:2344954/71‑1 (MQ=255)

                          aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                           >  1:3503669/1‑71 (MQ=255)

                          aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                           >  1:252344/1‑71 (MQ=255)

                           cGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCt                                          <  1:757314/71‑1 (MQ=255)

                             cccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTc                                        <  1:2782602/71‑1 (MQ=255)

                             cccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTc                                        <  1:1832359/71‑1 (MQ=255)

                              ccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCa                                       <  1:1882476/71‑1 (MQ=255)

                               ctgctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCa                                       >  1:588316/1‑70 (MQ=255)

                                tgctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATc                                     >  1:1826727/1‑71 (MQ=255)

                                 gctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGt                                            >  1:248784/1‑63 (MQ=255)

                                  ctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCtt                                   >  1:2083102/1‑71 (MQ=255)

                                     gCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:513243/71‑1 (MQ=255)

                                      cAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                               <  1:2253060/71‑1 (MQ=255)

                                       aGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                              >  1:1201744/1‑71 (MQ=255)

                                       aGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                              >  1:2742978/1‑71 (MQ=255)

                                        gTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAt                             <  1:221972/71‑1 (MQ=255)

                                         tGATTAGCCTGGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                           <  1:3697862/56‑1 (MQ=255)

                                          gATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAg                                                           >  1:1859674/1‑39 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTTATTgg                          <  1:1508524/71‑1 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          >  1:628321/1‑71 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          >  1:632635/1‑71 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          >  1:2621807/1‑71 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          <  1:1201628/71‑1 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          >  1:1860862/1‑71 (MQ=255)

                                           aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                          >  1:2114167/1‑71 (MQ=255)

                                                  tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                <  1:2437509/42‑1 (MQ=255)

                                                  tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                <  1:1917261/42‑1 (MQ=255)

                                                  tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCt                                    <  1:1503939/54‑1 (MQ=255)

                                                  tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAtt                            <  1:1425230/62‑1 (MQ=255)

                                                   cgcgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCt                                    <  1:1664158/53‑1 (MQ=255)

                                                    gcgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                           >  1:3377802/1‑61 (MQ=255)

                                                    gcgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCa                 <  1:3348654/71‑1 (MQ=255)

                                                     cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:3670116/55‑1 (MQ=255)

                                                     cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:3332355/55‑1 (MQ=255)

                                                     cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:3771807/55‑1 (MQ=255)

                                                     cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:2993588/55‑1 (MQ=255)

                                                      gATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTCTGTTGCATc               <  1:2141522/71‑1 (MQ=255)

                                                       aTGTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGAtt                            <  1:2016631/57‑1 (MQ=255)

                                                       aTGTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGAtt                            <  1:764536/57‑1 (MQ=255)

                                                       aTGTTCTTTCTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                              >  1:2561963/1‑55 (MQ=255)

                                                         gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                           >  1:1502012/1‑40 (MQ=38)

                                                         gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                           >  1:1341528/1‑40 (MQ=38)

                                                         gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                           >  1:625984/1‑40 (MQ=38)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTc                                  <  1:3038870/48‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTc                                  <  1:3402089/48‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                <  1:1085522/50‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                           <  1:942145/55‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                           <  1:2044070/55‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                           <  1:2048613/55‑1 (MQ=255)

                                                          ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAAc            >  1:3132558/1‑70 (MQ=255)

                                                           tCTTTGTTCCCATGCAGGCCAGCATCACTATGttt                                              <  1:1884280/35‑1 (MQ=255)

                                                           tCTTTGTTCCCATGCAGGCCAGCATCACTATGttt                                              <  1:894495/35‑1 (MQ=255)

                                                            cTTTGTTCCTATGCAGGCCAGCATTACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:54991/1‑71 (MQ=255)

                                                            cTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt           >  1:3798688/1‑69 (MQ=255)

                                                            cTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:391239/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGt                                            >  1:3670070/1‑36 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGt                                            >  1:2206314/1‑36 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                >  1:823631/1‑48 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                >  1:2824702/1‑48 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                              >  1:2123742/1‑50 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                           >  1:1616400/1‑53 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:2156112/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:758253/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:411978/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:1786963/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg         >  1:186728/1‑71 (MQ=255)

                                                            cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg          >  1:1338765/1‑70 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt        <  1:2938040/69‑1 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt        <  1:1209710/69‑1 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt        <  1:3737428/69‑1 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt        <  1:3385598/69‑1 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt        <  1:2754650/69‑1 (MQ=255)

                                                               tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGa      >  1:2450963/1‑71 (MQ=255)

                                                                 ttCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGac     <  1:2247574/70‑1 (MQ=255)

                                                                   cccATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACAcc  >  1:2968004/1‑71 (MQ=255)

                                                                   cccATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACAcc  >  1:3827924/1‑71 (MQ=255)

TAAAGTCACTATCTTCTGGTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACC  >  W3110S.gb/3805360‑3805497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`