BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,805,447	T→C	8.0%	T320T (ACT→ACC)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,805,447	0	T	C	8.0%	375.3 / 17.9	138	T320T (ACT→ACC)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (65/62); new base C (8/3); total (73/65)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 150 total aligned reads displayed.

GTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAATGATGT  >  W3110S.gb/3805378‑3805517

gtggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACt                                                                        <  1:429693/70‑1 (MQ=255)

 tggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTAt                                                                      <  1:3355498/71‑1 (MQ=255)

  ggACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATg                                                                     >  1:3043960/1‑71 (MQ=255)

   gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                                    >  1:1001013/1‑71 (MQ=255)

   gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                                    >  1:238193/1‑71 (MQ=255)

   gACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                                    <  1:1976779/71‑1 (MQ=255)

        aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                                               >  1:3503669/1‑71 (MQ=255)

        aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                                               >  1:252344/1‑71 (MQ=255)

        aCGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTc                                                               <  1:2344954/71‑1 (MQ=255)

           cccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTc                                                            <  1:2782602/71‑1 (MQ=255)

           cccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTc                                                            <  1:2166102/71‑1 (MQ=255)

            ccTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCa                                                           <  1:1882476/71‑1 (MQ=255)

              tgctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATc                                                         >  1:1826727/1‑71 (MQ=255)

                ctgGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCtt                                                       >  1:2083102/1‑71 (MQ=255)

                    cAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTg                                                   <  1:2253060/71‑1 (MQ=255)

                     aGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                                                  >  1:1201744/1‑71 (MQ=255)

                      gTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGAt                                                 <  1:221972/71‑1 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTc                                                            >  1:2589824/1‑57 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTTATTgg                                              <  1:1508524/71‑1 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                                              >  1:632635/1‑71 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                                              >  1:2114167/1‑71 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                                              >  1:2621807/1‑71 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                                              <  1:1201628/71‑1 (MQ=255)

                         aTTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTgg                                              >  1:1901441/1‑71 (MQ=255)

                             gCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgt                                           <  1:1758951/70‑1 (MQ=255)

                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                                    <  1:2437509/42‑1 (MQ=255)

                                tCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGt                                                                    <  1:1917261/42‑1 (MQ=255)

                                tCGCGATGTTCTTTGTTCACATGCAGGCCAGCATCACTATGTTTGTCTTCATCt                                                        <  1:2503741/54‑1 (MQ=255)

                                 cgcgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCt                                                        <  1:1664158/53‑1 (MQ=255)

                                  gcgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCa                                     <  1:3348654/71‑1 (MQ=255)

                                   cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    <  1:2993588/55‑1 (MQ=255)

                                   cgATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    <  1:3670116/55‑1 (MQ=255)

                                    gATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTCTGTTGCATc                                   <  1:2141522/71‑1 (MQ=255)

                                     aTGTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGAtt                                                <  1:764536/57‑1 (MQ=255)

                                     aTGTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGAtt                                                <  1:2016631/57‑1 (MQ=255)

                                       gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                                               >  1:625984/1‑40 (MQ=38)

                                       gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                                               >  1:1341528/1‑40 (MQ=38)

                                       gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                                               >  1:1502012/1‑40 (MQ=38)

                                       gTTCTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTc                                                               >  1:647217/1‑40 (MQ=38)

                                       gTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAAc                                >  1:3790471/1‑71 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTc                                                      <  1:3402089/48‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    <  1:3017326/50‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    <  1:1085522/50‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                                               <  1:1213206/55‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                                               <  1:2044070/55‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTg                                               <  1:942145/55‑1 (MQ=255)

                                        ttCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAAc                                >  1:3132558/1‑70 (MQ=255)

                                         tCTTTGTTCCCATGCAGGCCAGCATCACTATGttt                                                                  <  1:894495/35‑1 (MQ=255)

                                         tCTTTGTTCCCATGCAGGCCAGCATCACTATGttt                                                                  <  1:1884280/35‑1 (MQ=255)

                                         tCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGgtgt                                           <  1:1864635/58‑1 (MQ=255)

                                          cTTTGTTCCTATGCAGGCCAGCATTACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:54991/1‑71 (MQ=255)

                                          cTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACt                               >  1:3798688/1‑69 (MQ=255)

                                          cTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:391239/1‑71 (MQ=255)

                                          cTTTGTTCCTATGCAGGCCAGCATCACCATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:2917654/1‑71 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCtt                                                             >  1:39213/1‑39 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    >  1:2824702/1‑48 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGt                                                    >  1:823631/1‑48 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGa                                                  >  1:2123742/1‑50 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:411978/1‑71 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:758253/1‑71 (MQ=255)

                                          cTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTgg                             >  1:1786963/1‑71 (MQ=255)

                                           tttGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              <  1:1349167/69‑1 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt                            <  1:2754650/69‑1 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt                            <  1:3385598/69‑1 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt                            <  1:1209710/69‑1 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt                            <  1:2938040/69‑1 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGa                          >  1:2450963/1‑71 (MQ=255)

                                             tGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGa                          >  1:600993/1‑71 (MQ=255)

                                               ttCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGac                         <  1:2247574/70‑1 (MQ=255)

                                                    aTGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTTATTgg                                              >  1:3575577/1‑44 (MQ=255)

                                                    aTGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTa                    >  1:3763645/1‑70 (MQ=255)

                                                    aTGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTAt                   >  1:1539957/1‑71 (MQ=255)

                                                    aTGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTAt                   >  1:1579550/1‑71 (MQ=255)

                                                     tGCAGGCCAGCATCACTATGTTTGTCTTCATCTTc                                                      <  1:715400/35‑1 (MQ=255)

                                                     tGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATc                  >  1:601971/1‑71 (MQ=255)

                                                     tGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATc                  >  1:53951/1‑71 (MQ=255)

                                                       cAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATGAACTGGTGACACCTATcc                 <  1:622043/70‑1 (MQ=255)

                                                       cAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATcc                 <  1:1497729/70‑1 (MQ=255)

                                                         ggCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGt                            <  1:1864587/57‑1 (MQ=255)

                                                           ccAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              >  1:1825342/1‑53 (MQ=255)

                                                           ccAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              >  1:3097514/1‑53 (MQ=255)

                                                           ccAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGa                          >  1:3702713/1‑57 (MQ=255)

                                                                aTCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       >  1:2160411/1‑71 (MQ=255)

                                                                aTCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       <  1:3625981/71‑1 (MQ=255)

                                                                aTCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       <  1:746137/71‑1 (MQ=255)

                                                                aTCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       <  1:1840752/71‑1 (MQ=255)

                                                                aTCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       <  1:492166/71‑1 (MQ=255)

                                                                   aCTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATcc                 >  1:3299525/1‑58 (MQ=255)

                                                                   aCTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAatgat    >  1:3603253/1‑71 (MQ=255)

                                                                    cTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAatgatg   >  1:1838808/1‑71 (MQ=255)

                                                                    cTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       >  1:2758096/1‑67 (MQ=255)

                                                                    cTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAat       >  1:1953189/1‑67 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATc                                   <  1:3690787/38‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              <  1:3833145/43‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              <  1:1513916/43‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTg                              <  1:946140/43‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATcc                 <  1:167809/56‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGt              <  1:373132/59‑1 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAATGATGt  >  1:2377539/1‑71 (MQ=255)

                                                                     tATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAATGATGt  >  1:3744099/1‑71 (MQ=255)

GTGGACGAACGCCCTGCTGGCAGTGATTAGCCTCGCGATGTTCTTTGTTCCCATGCAGGCCAGCATCACTATGTTTGTCTTCATCTTCGTGATTGGTGTGTTGCATCAACTGGTGACACCTATCCAGTGGGTAATGATGT  >  W3110S.gb/3805378‑3805517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`