| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,410 | T→A | 100% | pseudogene (543/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,410 | 0 | T | A | 98.7% | 214.4 / ‑4.3 | 75 | pseudogene (543/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (38/36); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGG > W3110S.gb/3824342‑3824472 | gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc > 1:630595/1‑71 (MQ=255) cGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg > 1:1853139/1‑68 (MQ=255) ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc > 1:554003/1‑70 (MQ=255) ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc > 1:225258/1‑70 (MQ=255) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa > 1:3279704/1‑63 (MQ=255) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCg < 1:3199485/70‑1 (MQ=255) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:1063349/1‑71 (MQ=255) tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg < 1:2718677/71‑1 (MQ=255) ggtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa < 1:3193241/68‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:762581/69‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:2457143/69‑1 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:1168252/4‑69 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:276315/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:3175103/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:1240312/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:1388081/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTAAGCCGCAGAGACCGACATGAACGTAGt > 1:1776242/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATAAGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:1183083/4‑69 (MQ=255) taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt < 1:1857207/68‑1 (MQ=255) aaaCGGTGAACCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtg < 1:1078379/69‑1 (MQ=255) aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat < 1:907376/71‑1 (MQ=255) aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat < 1:1130282/71‑1 (MQ=255) gtgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa < 1:57526/55‑1 (MQ=255) tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:3128718/42‑1 (MQ=37) tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:506983/42‑1 (MQ=37) tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:2702629/42‑1 (MQ=37) tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCg < 1:330253/70‑1 (MQ=255) gAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa < 1:2556700/56‑1 (MQ=255) gAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa < 1:879190/56‑1 (MQ=255) aatttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACg > 1:298121/3‑71 (MQ=255) tttGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:3237565/35‑1 (MQ=255) tttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:3148883/1‑57 (MQ=255) tttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:362190/1‑57 (MQ=255) tttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:15530/65‑1 (MQ=255) tttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACggg > 1:1720791/1‑71 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt > 1:1721640/1‑50 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:1669028/1‑55 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:1793490/1‑55 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:901028/1‑55 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:467063/1‑55 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:2399681/1‑55 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgcg > 1:3746026/1‑71 (MQ=255) gATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt < 1:325975/47‑1 (MQ=255) gATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt < 1:2315496/47‑1 (MQ=255) tCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:39171/44‑1 (MQ=255) tCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:813733/44‑1 (MQ=255) tCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:2695107/44‑1 (MQ=255) tCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:2408848/44‑1 (MQ=255) tCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:2197058/58‑1 (MQ=255) cTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatcat > 1:1030242/1‑71 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:897119/1‑44 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:3095719/56‑1 (MQ=255) tgAATAGGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt < 1:3336341/41‑1 (MQ=255) ggAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatga > 1:910964/1‑50 (MQ=255) gAATATGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGtatca < 1:3701801/67‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:1818529/1‑46 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:3126387/1‑46 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatc < 1:3205387/66‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg > 1:3632285/1‑70 (MQ=255) aaTACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg > 1:18727/1‑69 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:1207571/51‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGa < 1:3492460/50‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGa < 1:2977033/50‑1 (MQ=255) aCGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGa < 1:230477/49‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTg < 1:1771647/70‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTg > 1:2074081/1‑70 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTg < 1:2147715/70‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGc < 1:1350181/71‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATAAACGTAGTGATGACCGaa > 1:2991675/1‑49 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATAAACGTAGTGATGACCGaa > 1:1349197/1‑49 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATAAACGTAGTGATGACCGaa > 1:3230523/1‑49 (MQ=255) gTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCa < 1:3715704/71‑1 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAggg > 1:2123540/1‑71 (MQ=255) aCTCAGCCGCAGAGACCGACATGAACGTAGtgatg > 1:2736285/1‑35 (MQ=255) aCTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatca > 1:3418304/1‑54 (MQ=255) | GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGG > W3110S.gb/3824342‑3824472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |