| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,924,649 | T→C | 7.0% | S191S (TCT→TCC) | bisC → | biotin sulfoxide reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,924,649 | 0 | T | C | 7.0% | 153.6 / 2.6 | 57 | S191S (TCT→TCC) | bisC | biotin sulfoxide reductase |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base C (2/2); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTAACCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCTT > W3110S.gb/3924582‑3924717 | cTAACCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCa < 1:3743087/71‑1 (MQ=255)cTAACCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCa > 1:3620678/1‑71 (MQ=255) aCTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGt > 1:1286281/1‑70 (MQ=255) tCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTg < 1:1172088/60‑1 (MQ=255) cAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGa > 1:125389/1‑69 (MQ=255) cAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGa > 1:637418/1‑69 (MQ=255) cAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGa > 1:875854/1‑69 (MQ=255) aaTACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCAc > 1:710315/1‑61 (MQ=255) aaTACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAg > 1:229161/1‑71 (MQ=255) aaTACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAg > 1:2091586/1‑71 (MQ=255) aCGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCgg > 1:1054019/1‑71 (MQ=255) aCGCTGAAAATTGCGTGGAATACATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCgg > 1:3794054/1‑71 (MQ=255) cGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCgg > 1:3020840/1‑70 (MQ=255) cTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaa < 1:603554/71‑1 (MQ=255) tGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCCGCACTGCg < 1:268783/58‑1 (MQ=255) tGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCCGCACTGCg < 1:3619763/58‑1 (MQ=255) aaaaTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCa > 1:879699/1‑51 (MQ=255) aaaaTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaaaa < 1:18898/70‑1 (MQ=255) aaaaTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaaa < 1:21378/69‑1 (MQ=255) aaaaTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaaa < 1:2373050/69‑1 (MQ=255) aaTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGc > 1:373902/1‑71 (MQ=255) aTTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCt < 1:1291959/71‑1 (MQ=255) cGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCCGCACTGCGTGACAGCGGGAAAAAGCTGATc > 1:1599275/1‑71 (MQ=255) cGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCCGCACTGCGTGACAGCGGGAAAAAGCTGATc > 1:2722735/1‑71 (MQ=255) gTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaa < 1:3319756/60‑1 (MQ=255) tGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCAtt > 1:1590588/1‑70 (MQ=255) tGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCAtt > 1:1390619/1‑70 (MQ=255) tGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTg < 1:1434412/71‑1 (MQ=255) ccGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATcc < 1:3204386/70‑1 (MQ=255) cGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGa > 1:3819227/1‑39 (MQ=255) cGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCa > 1:910890/1‑62 (MQ=255) gCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGAt < 1:1286081/71‑1 (MQ=255) aGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCg < 1:40409/71‑1 (MQ=255) aGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCg < 1:1825373/71‑1 (MQ=255) gggCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCgg > 1:1602765/1‑70 (MQ=255) gggCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGa < 1:347204/71‑1 (MQ=255) gCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGaaa < 1:1866329/37‑1 (MQ=255) cTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGa > 1:2530089/1‑68 (MQ=255) cTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAAc > 1:3595556/1‑71 (MQ=255) cTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAAc > 1:976129/1‑71 (MQ=255) ttCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTg < 1:3308797/40‑1 (MQ=255) tCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATc > 1:1110704/1‑42 (MQ=255) cTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGAt > 1:1198452/1‑40 (MQ=255) ttACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTg < 1:1658596/42‑1 (MQ=255) ttACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTc > 1:663991/1‑70 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCa > 1:911113/1‑42 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATTCAATGCGATCGGAAACCGTCGAt > 1:598430/1‑71 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCg < 1:2592041/69‑1 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCg < 1:2363061/69‑1 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt > 1:2697367/1‑71 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt > 1:1658473/1‑71 (MQ=255) aCTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt > 1:977743/1‑71 (MQ=255) ttttCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt < 1:3064603/69‑1 (MQ=255) ttttCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt < 1:2850073/69‑1 (MQ=255) ttttCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGAt < 1:857409/69‑1 (MQ=255) ttCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCt > 1:3074618/1‑71 (MQ=255) cTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCtt < 1:1392883/70‑1 (MQ=255) | CTAACCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACAGCGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCTT > W3110S.gb/3924582‑3924717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |