| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 363,556 | C→T | 13.9% | A658A (GCG→GCA) | lacZ ← | beta‑D‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 363,556 | 0 | C | T | 13.9% | 85.7 / 6.1 | 36 | A658A (GCG→GCA) | lacZ | beta‑D‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base C (16/15); new base T (3/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGG > W3110S.gb/363491‑363623 | aaCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGccac > 1:3598050/1‑71 (MQ=255) tttACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATc < 1:924493/70‑1 (MQ=255) ttGTGGAGCGACATCGAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGc < 1:218337/70‑1 (MQ=255) ttGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTg > 1:3592426/1‑69 (MQ=255) ttGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGc > 1:1069537/1‑70 (MQ=255) cGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCg > 1:3700842/1‑71 (MQ=255) gACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCa > 1:3029142/1‑57 (MQ=255) cATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa < 1:3613852/71‑1 (MQ=255) cATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa < 1:2998478/71‑1 (MQ=255) cAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATc > 1:1790683/1‑49 (MQ=255) cAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTAt > 1:3803948/1‑68 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:1344680/1‑70 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:3628319/1‑70 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:1419361/1‑70 (MQ=255) ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCAt < 1:2587699/39‑1 (MQ=255) cACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:2337708/1‑70 (MQ=255) cACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCAACCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:2798856/1‑70 (MQ=255) aCCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACa < 1:3839739/71‑1 (MQ=255) ccGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACGGaa < 1:1603659/68‑1 (MQ=255) ccGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACGGaa < 1:491466/68‑1 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCg < 1:1924078/57‑1 (MQ=255) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAgg < 1:1035945/71‑1 (MQ=255) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAgg < 1:472801/71‑1 (MQ=255) gCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa > 1:2463070/1‑52 (MQ=255) tGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAAc < 1:1877245/64‑1 (MQ=255) gCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTc > 1:1189472/1‑71 (MQ=255) gCGGCTTACCATCCAGCTCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTg > 1:1989846/1‑71 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:3347388/1‑50 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa < 1:3197672/56‑1 (MQ=255) cATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCg < 1:471813/71‑1 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATgt < 1:1494267/43‑2 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa > 1:379380/1‑54 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa > 1:1853797/1‑54 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg > 1:3519193/1‑71 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg < 1:2829476/71‑1 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg < 1:1505936/71‑1 (MQ=255) | AACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGG > W3110S.gb/363491‑363623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |