| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,946,443 | T→C | 10.2% | D421D (GAT→GAC) | bcsA → | cellulose synthase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,946,443 | 0 | T | C | 10.2% | 96.3 / 3.3 | 39 | D421D (GAT→GAC) | bcsA | cellulose synthase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/21); new base C (2/2); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TGAACGCAACCTGGGGCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAGCC > W3110S.gb/3946374‑3946505 | tGAACGCAACCTGGGGCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATg > 1:3568894/1‑71 (MQ=255) aCGCAACCTGGGGCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGc > 1:1950107/1‑70 (MQ=255) gCAACCTGGGGCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAAc < 1:721395/71‑1 (MQ=255) ggCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:1244162/71‑1 (MQ=255) ggCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:1262467/71‑1 (MQ=255) ggCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:2631458/71‑1 (MQ=255) ggCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:729618/71‑1 (MQ=255) ggCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:2888407/71‑1 (MQ=255) tttCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAAc < 1:3436462/58‑1 (MQ=255) tttCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGGCTGGTGCAGGATGGCAACGATATGTgg < 1:1313708/67‑1 (MQ=255) tttCCGTAAAACACCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGACGGCAATGATATGTGGGa < 1:3202443/69‑1 (MQ=255) tttCCGTAAAACACAGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGACGGCAATGATATGTGGGa < 1:3780175/69‑1 (MQ=255) tCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATAtg > 1:2421328/1‑62 (MQ=255) tCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATAtg > 1:3117790/1‑62 (MQ=255) tCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGc > 1:1082822/1‑70 (MQ=255) tCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGc > 1:1905689/1‑70 (MQ=255) cGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCa < 1:349554/70‑1 (MQ=255) cGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCa < 1:228993/70‑1 (MQ=255) cGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCAc > 1:132231/1‑71 (MQ=255) gTAAAACACCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGACGGCaat > 1:2316110/1‑52 (MQ=38) gTAAAACACCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGACGGCa > 1:1767524/1‑51 (MQ=38) tAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTgg < 1:1469350/61‑1 (MQ=255) aaaaCGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACtt > 1:2659440/1‑70 (MQ=255) cGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAAc > 1:1554657/1‑47 (MQ=255) cgaacgaaGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGa < 1:238571/55‑1 (MQ=255) aaGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTcc < 1:3261608/71‑1 (MQ=255) aGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTcc < 1:187815/70‑1 (MQ=255) ggCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCtg < 1:2003653/71‑1 (MQ=255) gCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTcc < 1:134968/68‑1 (MQ=255) cGCTGTTCTATGGTCTGGTGCATGATGGCAACGATACGTGGGACGCCACTttc < 1:2194949/53‑1 (MQ=39) cGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCg > 1:1071930/1‑71 (MQ=255) cTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATtc > 1:608429/1‑71 (MQ=255) tGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATtcg < 1:428034/69‑1 (MQ=255) tGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATtcg < 1:3727939/69‑1 (MQ=255) tGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAg > 1:872812/1‑71 (MQ=255) tGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAg > 1:1768953/1‑71 (MQ=255) gTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAGcc > 1:38376/1‑71 (MQ=255) gCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTcc < 1:33121/46‑1 (MQ=255) gCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTcc < 1:58881/46‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCt > 1:3380280/1‑41 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCt > 1:3367871/1‑41 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCt > 1:840656/1‑41 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGa < 1:2226013/50‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGa < 1:1574460/50‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGa < 1:1350873/50‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGa < 1:2801423/50‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGa < 1:3013227/50‑1 (MQ=255) tGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAGc > 1:2982726/1‑62 (MQ=255) | TGAACGCAACCTGGGGCGTTTCCGTAAAACGCCGAACGAAGGCACGCTGTTCTATGGTCTGGTGCAGGATGGCAACGATATGTGGGACGCCACTTTCTTCTGCGGTTCCTGTGCGGTGATTCGTCGTAAGCC > W3110S.gb/3946374‑3946505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |