| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,977,928 | T→C | 8.5% | K692K (AAA→AAG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,977,928 | 0 | T | C | 8.5% | 120.5 / 3.0 | 47 | K692K (AAA→AAG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (28/15); new base C (2/2); total (30/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
CGTACCCCGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAG > W3110S.gb/3977860‑3977995 | cGTACCCCGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGttt < 1:2873516/71‑1 (MQ=255) gTACCCCGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGtttt > 1:1305358/1‑71 (MQ=255) tACCCCGGTGCCCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGttttt > 1:633177/1‑71 (MQ=255) tACCCCGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGttttt > 1:2623928/1‑71 (MQ=255) ccccGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCg < 1:1706895/71‑1 (MQ=255) ccccGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCg > 1:3467057/1‑71 (MQ=255) ccccGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCg < 1:3291391/71‑1 (MQ=255) ccccGATGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTCAGCTTTTc < 1:602266/70‑1 (MQ=255) ccccGATGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTCAGCTTTTc < 1:2790941/70‑1 (MQ=255) gTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGccc > 1:71195/1‑71 (MQ=255) gTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGccc > 1:1859657/1‑71 (MQ=255) tGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGcccc < 1:3858687/71‑1 (MQ=255) gACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGccg > 1:2105824/1‑68 (MQ=255) gACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGccc > 1:239427/1‑69 (MQ=255) aCCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAg > 1:1263247/1‑71 (MQ=255) aTTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGtttt < 1:2717809/55‑1 (MQ=255) ggTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGt < 1:881472/70‑1 (MQ=255) tGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAgg > 1:3463741/1‑61 (MQ=255) gATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTc > 1:1252826/1‑71 (MQ=255) gATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTCAGCTTTTCGTGCCCCAGATTGCCGTTGTc > 1:1684758/1‑71 (MQ=255) gATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTCAGCTTTTCGTGCCCCAGATTGCCGTTGTc > 1:3803459/1‑71 (MQ=255) aTTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTc < 1:1497718/70‑1 (MQ=255) tGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTc > 1:1690597/1‑47 (MQ=255) tGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTc > 1:167273/1‑47 (MQ=255) tGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcag > 1:2002897/1‑71 (MQ=255) tGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcag > 1:421826/1‑71 (MQ=255) tGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCca > 1:2209766/1‑70 (MQ=255) gcgcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCg < 1:463355/62‑1 (MQ=255) gcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGtt > 1:1064191/1‑62 (MQ=255) cAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa < 1:3030325/71‑1 (MQ=255) tGATAACAGCTCGTTTCGCGCCTTGGTTAGttttt < 1:520778/35‑1 (MQ=255) gATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTa > 1:964387/1‑48 (MQ=255) gATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca > 1:2187229/1‑61 (MQ=255) gATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCatat > 1:3507944/1‑70 (MQ=255) gATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCatat > 1:564360/1‑70 (MQ=255) aTAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATc > 1:1064495/1‑70 (MQ=255) aTAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATc > 1:212551/1‑70 (MQ=255) tAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa < 1:3316151/65‑1 (MQ=255) tAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaa < 1:1136006/71‑1 (MQ=255) tAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaa < 1:1776054/71‑1 (MQ=255) aaCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa > 1:2381114/1‑64 (MQ=255) aaCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa > 1:3493192/1‑64 (MQ=255) aCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCat < 1:1179016/64‑1 (MQ=255) aGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGccc < 1:2451898/35‑1 (MQ=255) tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc < 1:310806/71‑1 (MQ=255) tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc < 1:3000867/71‑1 (MQ=255) tCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCgg > 1:3763907/1‑71 (MQ=255) gcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagc > 1:2747453/1‑42 (MQ=255) gcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaaaa > 1:323382/1‑57 (MQ=255) gggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGt > 1:2200884/1‑71 (MQ=255) gTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaa < 1:128896/49‑1 (MQ=255) tAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGtt > 1:225434/1‑68 (MQ=255) tAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAg > 1:1836055/1‑71 (MQ=255) tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGtt < 1:2567226/65‑1 (MQ=255) tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGtt < 1:3186105/65‑1 (MQ=255) | CGTACCCCGGTGACCTGATTCGGTGATTGCGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAG > W3110S.gb/3977860‑3977995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |