| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,993,747 | C→T | 9.1% | E124E (GAG→GAA) | gor ← | glutathione oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,993,747 | 0 | C | T | 9.1% | 184.5 / 6.8 | 66 | E124E (GAG→GAA) | gor | glutathione oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (27/33); new base T (4/2); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
TCCGGGTGGCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATCAACGTTATTTTTACC > W3110S.gb/3993679‑3993809 | tCCGGGTGGCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTc > 1:3257550/1‑71 (MQ=255) gTGGCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCg < 1:2425616/71‑1 (MQ=255) gTGGCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCg < 1:1938193/71‑1 (MQ=255) ggCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGtt > 1:800332/1‑71 (MQ=255) gCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGttt > 1:3550524/1‑71 (MQ=255) cTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGtttt < 1:1437645/71‑1 (MQ=255) ggACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTgg < 1:3280775/70‑1 (MQ=255) cgacCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATc < 1:2171893/71‑1 (MQ=255) ccTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGGATCAACg > 1:1097413/1‑69 (MQ=255) ccTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCa > 1:2792454/1‑66 (MQ=255) ccTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACg > 1:627142/1‑69 (MQ=255) tgGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCgg > 1:2269917/1‑71 (MQ=255) tgGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCgg > 1:3130803/1‑71 (MQ=255) tgGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCgg > 1:3135260/1‑71 (MQ=255) tgGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCgg > 1:214867/1‑71 (MQ=255) ggCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCggg > 1:2462541/1‑71 (MQ=255) ggCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCggg > 1:835196/1‑71 (MQ=255) aTCAGAATATGATCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCa > 1:2643558/1‑69 (MQ=255) aTCAGAATATGATCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCa > 1:764875/1‑69 (MQ=255) aTCAGAATATGATCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCa > 1:1785709/1‑69 (MQ=255) aTCAGAATATGATCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCa > 1:1688125/1‑69 (MQ=255) aaTATGATCGGCCGTTATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCt < 1:551661/70‑1 (MQ=255) atatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt > 1:244156/1‑71 (MQ=255) tatGATCGTCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATc < 1:2048651/49‑1 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGtt < 1:3779571/41‑1 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGtt < 1:1667436/41‑1 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGtt < 1:3678455/41‑1 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:896979/70‑1 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt > 1:2489130/1‑70 (MQ=255) tatGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:1824488/70‑1 (MQ=255) tGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAt < 1:1452012/71‑1 (MQ=255) tGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAt < 1:2998147/71‑1 (MQ=255) tGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAt < 1:1556449/71‑1 (MQ=255) tGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAt > 1:3468818/1‑71 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGa > 1:3814546/1‑68 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGa > 1:377147/1‑68 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAtt > 1:712332/1‑70 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTa < 1:3051854/71‑1 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAtt < 1:54105/70‑1 (MQ=255) aTCGGCCGTGATGGTTTCGCCGTTGACTTCAATGGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAtt < 1:848375/70‑1 (MQ=255) cGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTAc > 1:2167568/1‑70 (MQ=255) gCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATc < 1:725489/71‑1 (MQ=255) gCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATc < 1:2182336/71‑1 (MQ=255) gTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGaa > 1:3377046/1‑44 (MQ=255) tGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTa < 1:275329/63‑1 (MQ=255) gATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTg < 1:1678428/58‑1 (MQ=255) gATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTg < 1:1372405/58‑1 (MQ=255) gATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTa < 1:1483344/62‑1 (MQ=255) aTGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAg < 1:1026247/51‑1 (MQ=255) tGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGAt > 1:3318011/1‑58 (MQ=255) gTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCa < 1:1444141/45‑1 (MQ=255) gTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCa < 1:314291/45‑1 (MQ=255) gTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCa < 1:2547313/45‑1 (MQ=255) tttCGCCGTTTACCTCCAGCGTTTTGGCATCAACGa > 1:3837580/1‑36 (MQ=255) ttCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCg > 1:3647604/1‑39 (MQ=255) ttCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCg > 1:3110110/1‑39 (MQ=255) ttCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCg > 1:601396/1‑39 (MQ=255) tCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATCAACGTTAtttt > 1:2703244/1‑70 (MQ=255) cGTTTACCTCCAGCGTTTTGGCATCTACGAAGCTGGCAAAGCCttt < 1:645638/46‑1 (MQ=255) cGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:1411070/46‑1 (MQ=255) cGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:1829560/46‑1 (MQ=255) cGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGACTTTGATTACATCAACGTTATTTTTAcc < 1:737883/70‑1 (MQ=255) gTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:1621125/45‑1 (MQ=255) gTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATCAACGTTATTTTTAcc > 1:551637/1‑69 (MQ=255) tttACCTCCAGCGTTTTGTCATCAACGAAGCGGGCAAAGCCttt < 1:3343845/44‑1 (MQ=255) ttACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCttt < 1:3172461/43‑1 (MQ=255) | TCCGGGTGGCTCGGACGACCGCCTGTGGCGATCAGAATATGATCGGCCGTGATGGTTTCGCCGTTTACCTCCAGCGTTTTGGCATCAACGAAGCGGGCAAAGCCTTTGATTACATCAACGTTATTTTTACC > W3110S.gb/3993679‑3993809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |