| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 363,576 | G→A | 12.1% | L652F (CTC→TTC) | lacZ ← | beta‑D‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 363,576 | 0 | G | A | 12.1% | 106.8 / 4.8 | 41 | L652F (CTC→TTC) | lacZ | beta‑D‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base G (13/23); new base A (3/2); total (16/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGG > W3110S.gb/363510‑363646 | cGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCg > 1:3700842/1‑71 (MQ=255) cATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa < 1:3613852/71‑1 (MQ=255) cATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa < 1:2998478/71‑1 (MQ=255) cAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTAt > 1:3803948/1‑68 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:3628319/1‑70 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:1344680/1‑70 (MQ=255) ttCACCGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACgg > 1:1419361/1‑70 (MQ=255) cACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:2337708/1‑70 (MQ=255) cACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCAACCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:2798856/1‑70 (MQ=255) aCCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACa < 1:3839739/71‑1 (MQ=255) ccGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACGGaa < 1:1603659/68‑1 (MQ=255) ccGCTTGCCAGCGGCTTGCCATCCAGTGCCACCATCCAGTGCAGGAACTCGTTATCGCTATGACGGaa < 1:491466/68‑1 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCg < 1:1924078/57‑1 (MQ=255) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAgg < 1:472801/71‑1 (MQ=255) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAgg < 1:1035945/71‑1 (MQ=255) gCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTa > 1:2463070/1‑52 (MQ=255) tGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAAc < 1:1877245/64‑1 (MQ=255) gCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTc > 1:1189472/1‑71 (MQ=255) gCGGCTTACCATCCAGCTCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTg > 1:1989846/1‑71 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGaa > 1:3347388/1‑50 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa < 1:3197672/56‑1 (MQ=255) cATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCg < 1:471813/71‑1 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATgt < 1:1494267/43‑2 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa > 1:1853797/1‑54 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa > 1:379380/1‑54 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg < 1:1505936/71‑1 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg < 1:2829476/71‑1 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATgg > 1:3519193/1‑71 (MQ=255) accaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGa > 1:22415/1‑61 (MQ=255) accaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGa > 1:2226621/1‑61 (MQ=255) aTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGa < 1:2728967/71‑1 (MQ=255) cAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCt < 1:953135/44‑1 (MQ=255) cAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGCTTTGCCCGGATa < 1:240191/70‑1 (MQ=255) gTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATaa < 1:3662932/69‑1 (MQ=255) tGCAGGAGCTCGTTAT‑‑CTATGACGGAACAGGTATTCGCTGGTc < 1:3427355/43‑1 (MQ=38) cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACgg < 1:2105073/70‑1 (MQ=255) cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACgg < 1:782867/70‑1 (MQ=255) cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGa < 1:827523/71‑1 (MQ=255) cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGa < 1:844667/71‑1 (MQ=255) cAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGa < 1:1126854/71‑1 (MQ=255) aGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTg < 1:3440801/71‑1 (MQ=255) gCTCGTTATCGCTATGACTGAACAGGTATTCGCTGGTCACTTc < 1:3576386/43‑1 (MQ=255) gCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTc < 1:2377449/43‑1 (MQ=255) gCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTc < 1:1127156/43‑1 (MQ=255) gCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATaaa > 1:55754/1‑62 (MQ=255) gCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTgg < 1:3548783/71‑1 (MQ=255) | CGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGG > W3110S.gb/363510‑363646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |