| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,041,961 | C→T | 6.5% | intergenic (+99/+89) | livJ → / ← yhhK | leucine/isoleucine/valine transporter subunit/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,041,961 | 0 | C | T | 6.5% | 183.2 / 2.1 | 62 | intergenic (+99/+89) | livJ/yhhK | leucine/isoleucine/valine transporter subunit/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (32/25); new base T (2/2); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
AAATTGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTT > W3110S.gb/4041892‑4042031 | aaaTTGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATcc < 1:2765304/71‑1 (MQ=255)aaaTTGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATcc < 1:2740653/71‑1 (MQ=255) aaTTGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCa < 1:3431640/71‑1 (MQ=255) ttGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCaaa < 1:480475/71‑1 (MQ=255) gCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCaaa < 1:3540448/69‑1 (MQ=255) gCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAg < 1:2447318/70‑1 (MQ=255) atatCGAGTACGTTGCTTCATGCCGGATTCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAAt > 1:962036/1‑70 (MQ=255) atatCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAAt < 1:1554038/70‑1 (MQ=255) gAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTa > 1:3042304/1‑70 (MQ=255) gAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTa < 1:3467818/70‑1 (MQ=255) gAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTa < 1:703198/70‑1 (MQ=255) gAGTACGTTGCTTCAAGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTa < 1:2255029/70‑1 (MQ=255) gCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAaca > 1:563416/1‑71 (MQ=255) gCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAaca > 1:1911924/1‑71 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCGTACAAGATCCAAAGAAATCAGTATATTGCAACACACATTg < 1:1739856/70‑1 (MQ=37) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTg < 1:3488744/70‑1 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTg < 1:549171/70‑1 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACAtt > 1:2218351/1‑69 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTg > 1:1417776/1‑70 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCt > 1:1069109/1‑71 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAacac < 1:1109863/56‑1 (MQ=255) gcgTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTGTa > 1:2380531/1‑63 (MQ=37) gcgTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTGTa > 1:3669056/1‑63 (MQ=37) tAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGt < 1:1815746/40‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTaaa > 1:716220/1‑41 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTaaa > 1:1321158/1‑41 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTaaa > 1:1413622/1‑41 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCa > 1:1800547/1‑46 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCa > 1:3664427/1‑46 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg > 1:3537195/1‑71 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:3619841/71‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg > 1:3780058/1‑71 (MQ=255) aCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAg < 1:915647/36‑1 (MQ=255) aCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAg < 1:1483299/36‑1 (MQ=255) aCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGt < 1:271361/71‑1 (MQ=255) cGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAAcacaca > 1:1712232/1‑51 (MQ=255) cTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAgcg > 1:3195828/1‑71 (MQ=255) ttATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAgcg > 1:3064916/1‑70 (MQ=255) aTCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAAcacaca > 1:686924/1‑45 (MQ=255) aTCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCAt > 1:702827/1‑71 (MQ=255) tCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTg < 1:3776046/47‑1 (MQ=255) tCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCAt > 1:2593400/1‑70 (MQ=255) cGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTa > 1:897941/1‑47 (MQ=255) gCCTACAAGATGCAAAGAAATCAGTAAATTGCAACACACAt < 1:1917016/41‑1 (MQ=255) gCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGc < 1:1090155/71‑1 (MQ=255) ccTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCa < 1:2195639/71‑1 (MQ=255) cTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa < 1:2793776/71‑1 (MQ=255) tACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATaa < 1:2574454/52‑1 (MQ=255) tACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATaa < 1:2831936/52‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAg < 1:124760/42‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:979619/54‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:1066536/54‑1 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACAt > 1:1535589/1‑36 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:2459931/1‑71 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:102832/1‑71 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTg < 1:3787279/37‑1 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAg > 1:3564275/1‑55 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:634801/1‑70 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAata > 1:1698581/1‑69 (MQ=255) aGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCa > 1:923871/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2985848/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2981288/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2234704/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATT‑TAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACttt > 1:2905439/1‑71 (MQ=255) | AAATTGCTAATATCGAGTACGTTGCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTT > W3110S.gb/4041892‑4042031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |