| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,041,983 | A→G | 7.1% | intergenic (+121/+67) | livJ → / ← yhhK | leucine/isoleucine/valine transporter subunit/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,041,983 | 0 | A | G | 7.1% | 145.6 / 2.7 | 56 | intergenic (+121/+67) | livJ/yhhK | leucine/isoleucine/valine transporter subunit/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (32/20); new base G (2/2); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGATTAA > W3110S.gb/4041915‑4042053 | gCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAaca > 1:563416/1‑71 (MQ=255)gCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAaca > 1:1911924/1‑71 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCGTACAAGATCCAAAGAAATCAGTATATTGCAACACACATTg < 1:1739856/70‑1 (MQ=37) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTg < 1:3488744/70‑1 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTg < 1:549171/70‑1 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACAtt > 1:2218351/1‑69 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTg > 1:1417776/1‑70 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCt > 1:1069109/1‑71 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAacac < 1:1109863/56‑1 (MQ=255) gcgTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTGTa > 1:2380531/1‑63 (MQ=37) gcgTAAACGCCTTATCCGGCCTACAAGATTCAAAGAAATCAGTAAATTGCAGCACACATTGTa > 1:3669056/1‑63 (MQ=37) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg > 1:3780058/1‑71 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg > 1:3537195/1‑71 (MQ=255) aaCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:3619841/71‑1 (MQ=255) aCGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGt < 1:271361/71‑1 (MQ=255) cGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAAcacaca > 1:1712232/1‑51 (MQ=255) cTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAgcg > 1:3195828/1‑71 (MQ=255) ttATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAgcg > 1:3064916/1‑70 (MQ=255) aTCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAAcacaca > 1:686924/1‑45 (MQ=255) aTCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCAt > 1:702827/1‑71 (MQ=255) tCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTg < 1:3776046/47‑1 (MQ=255) tCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCAt > 1:2593400/1‑70 (MQ=255) cGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTa > 1:897941/1‑47 (MQ=255) gCCTACAAGATGCAAAGAAATCAGTAAATTGCAACACACAt < 1:1917016/41‑1 (MQ=255) gCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGc < 1:1090155/71‑1 (MQ=255) ccTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCa < 1:2195639/71‑1 (MQ=255) cTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa < 1:2793776/71‑1 (MQ=255) tACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATaa < 1:2574454/52‑1 (MQ=255) tACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATaa < 1:2831936/52‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAg < 1:124760/42‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:1066536/54‑1 (MQ=255) aCAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCg < 1:979619/54‑1 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACAt > 1:1535589/1‑36 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:102832/1‑71 (MQ=255) cAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:2459931/1‑71 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTg < 1:3787279/37‑1 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAg > 1:3564275/1‑55 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAatat > 1:634801/1‑70 (MQ=255) aaGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAata > 1:1698581/1‑69 (MQ=255) aGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCa > 1:923871/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2981288/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2234704/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACtt > 1:2985848/1‑71 (MQ=255) tCCAAAGAAATCAGTAAATTGCAACACACATT‑TAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACttt > 1:2905439/1‑71 (MQ=255) aaGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCa > 1:3766434/1‑54 (MQ=255) aaGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa > 1:2282761/1‑59 (MQ=255) aaGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa > 1:3112238/1‑59 (MQ=255) aaGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa > 1:2573606/1‑59 (MQ=255) aGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAata > 1:146085/1‑60 (MQ=255) aGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGa > 1:3030660/1‑70 (MQ=255) aGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAAc > 1:1235421/1‑71 (MQ=255) tAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa < 1:140610/49‑1 (MQ=255) tAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACtt < 1:28679/71‑1 (MQ=255) aaTTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGa > 1:3722345/1‑71 (MQ=255) aaTTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGa > 1:3391030/1‑71 (MQ=255) aTTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGAc < 1:729529/71‑1 (MQ=255) cAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGATGAtt < 1:1429910/71‑1 (MQ=255) cAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGAtt < 1:3251134/71‑1 (MQ=255) cAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGAtt < 1:659661/71‑1 (MQ=255) acacacATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGATTaa < 1:2486113/71‑1 (MQ=255) acacacATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGATTaa < 1:705471/71‑1 (MQ=255) acacacATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGATTaa < 1:2557014/71‑1 (MQ=255) | GCTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAGATCCAAAGAAATCAGTAAATTGCAACACACATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATATCACTTTTGAAATCAAACTTGACGATTAA > W3110S.gb/4041915‑4042053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |