| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,077,898 | C→A | 14.9% | R169R (CGG→CGT) | glpD ← | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,077,898 | 0 | C | A | 14.9% | 114.8 / 5.4 | 40 | R169R (CGG→CGT) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (19/15); new base A (3/3); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
CCGGTATCGATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCG > W3110S.gb/4077830‑4077964 | ccGGTATCGATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTcc < 1:144015/70‑1 (MQ=255) tCGATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAg > 1:2941163/1‑71 (MQ=255) tCGATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAg > 1:2633078/1‑71 (MQ=255) gATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCa > 1:2415370/1‑71 (MQ=255) gATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGAGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCa > 1:3555811/1‑71 (MQ=255) tatCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACt < 1:815026/71‑1 (MQ=255) tCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACtt > 1:163556/1‑70 (MQ=255) ttCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcac > 1:3604815/1‑69 (MQ=255) ccGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccg > 1:1650660/1‑71 (MQ=255) cGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccgc < 1:391246/71‑1 (MQ=255) cGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccgc < 1:1452180/71‑1 (MQ=255) ccACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCtt < 1:3646184/69‑1 (MQ=255) cacaATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTCTAc > 1:262292/1‑71 (MQ=255) caATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACg > 1:3268436/1‑70 (MQ=255) tCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACt < 1:1078907/53‑1 (MQ=255) cacaGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGcaccac > 1:1074314/1‑71 (MQ=255) acaGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCaccacc < 1:34800/71‑1 (MQ=255) caGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTa > 1:2806608/1‑61 (MQ=255) caGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCAccacca < 1:887942/71‑1 (MQ=255) ccGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTg < 1:1391692/71‑1 (MQ=255) ttttCG‑GGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCAt < 1:2117664/64‑1 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCg > 1:3555413/1‑70 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:28418/1‑71 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:1449394/1‑71 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:146989/1‑71 (MQ=255) gCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTAc > 1:1019120/1‑71 (MQ=255) gagGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAg < 1:646686/71‑1 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:2585903/3‑60 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:65758/3‑60 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:2800053/3‑60 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg > 1:3258209/1‑71 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg > 1:2353448/1‑71 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg < 1:2609874/71‑1 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:191594/69‑1 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:2824523/69‑1 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:1981201/69‑1 (MQ=255) gcgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcg > 1:3721202/1‑70 (MQ=255) cgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtc < 1:1887374/71‑1 (MQ=255) cgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtc < 1:268303/71‑1 (MQ=255) gAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtcg < 1:1768351/71‑1 (MQ=255) gAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtcg < 1:2445981/71‑1 (MQ=255) | CCGGTATCGATATCTTCCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCG > W3110S.gb/4077830‑4077964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |