| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,077,913 | G→A | 15.7% | G164G (GGC→GGT) | glpD ← | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,077,913 | 0 | G | A | 15.7% | 88.3 / 8.0 | 38 | G164G (GGC→GGT) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (19/13); new base A (3/3); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGTCAG > W3110S.gb/4077846‑4077980 | ccGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccg > 1:1650660/1‑71 (MQ=255) cGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccgc < 1:391246/71‑1 (MQ=255) cGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTcgccgc < 1:1452180/71‑1 (MQ=255) ccACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCtt < 1:3646184/69‑1 (MQ=255) cacaATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTCTAc > 1:262292/1‑71 (MQ=255) caATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACg > 1:3268436/1‑70 (MQ=255) cacaGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGcaccac > 1:1074314/1‑71 (MQ=255) acaGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCaccacc < 1:34800/71‑1 (MQ=255) caGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTa > 1:2806608/1‑61 (MQ=255) caGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCAccacca < 1:887942/71‑1 (MQ=255) ccGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTg < 1:1391692/71‑1 (MQ=255) ttttCG‑GGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCAt < 1:2117664/64‑1 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCg > 1:3555413/1‑70 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:1449394/1‑71 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:146989/1‑71 (MQ=255) ttCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGt > 1:28418/1‑71 (MQ=255) gCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTAc > 1:1019120/1‑71 (MQ=255) gagGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAg < 1:646686/71‑1 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:65758/3‑60 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:2585903/3‑60 (MQ=255) acgTGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTgg > 1:2800053/3‑60 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg > 1:2353448/1‑71 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg < 1:2609874/71‑1 (MQ=255) gTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACg > 1:3258209/1‑71 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:2824523/69‑1 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:191594/69‑1 (MQ=255) tGGCACGAGTACGAGTAAGCACTTCACCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGAc < 1:1981201/69‑1 (MQ=255) gcgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcg > 1:3721202/1‑70 (MQ=255) cgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtc < 1:268303/71‑1 (MQ=255) cgAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtc < 1:1887374/71‑1 (MQ=255) gAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtcg < 1:1768351/71‑1 (MQ=255) gAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtcg < 1:2445981/71‑1 (MQ=255) cGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTg < 1:3246182/38‑1 (MQ=255) gTAAGCACTTCGCCGCCTTTACGCACCACCATCTg > 1:1704464/1‑35 (MQ=255) gTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCa < 1:2554438/71‑1 (MQ=255) aGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCaa > 1:2890365/1‑69 (MQ=255) aCTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGt > 1:644966/1‑70 (MQ=255) aCTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGt > 1:208728/1‑70 (MQ=255) tCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGcgtcg > 1:2597031/1‑54 (MQ=255) tCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGTCAg > 1:290769/1‑70 (MQ=255) tCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGTCAg > 1:2268543/1‑70 (MQ=255) | CCGCTTCCACAATCCACAGGCCGTTTTCGCGGCGAGCAGAGGTGGCGCGAGTCCGAGTAAGCACTTCGCCGCCTTTACGCACCACCATCTGGGCGTTGGCGAGTACCAGACGGGCGTCGTCTACCCAACAGTCAG > W3110S.gb/4077846‑4077980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |