| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,080,396 | T→C | 10.2% | I195I (ATT→ATC) | glpR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,080,396 | 0 | T | C | 10.2% | 98.5 / 3.4 | 39 | I195I (ATT→ATC) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (16/19); new base C (2/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01 | |||||||||||
AGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAAT > W3110S.gb/4080328‑4080465 | aGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatta < 1:953577/70‑1 (MQ=255) cGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTg < 1:172858/71‑1 (MQ=255) ggCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTga < 1:1952193/71‑1 (MQ=255) gCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTgag < 1:932772/71‑1 (MQ=255) aTCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGaa > 1:2489094/1‑71 (MQ=255) tCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:1002928/1‑71 (MQ=255) gATAGCTACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACt < 1:3266561/70‑1 (MQ=255) gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatta > 1:1888369/1‑61 (MQ=255) gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatta > 1:210971/1‑61 (MQ=255) gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTc > 1:1689756/1‑71 (MQ=255) ggCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGt < 1:231037/71‑1 (MQ=255) ggCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGt < 1:658894/71‑1 (MQ=255) gCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGt < 1:3503623/70‑1 (MQ=255) tCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTgag < 1:2624584/54‑1 (MQ=255) gctgGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCattatt < 1:196096/46‑1 (MQ=255) ctgGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatt < 1:1312906/42‑1 (MQ=255) ctgGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatt < 1:2728051/42‑1 (MQ=255) aGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTc < 1:128254/71‑1 (MQ=255) ttCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGc < 1:892745/61‑1 (MQ=255) ttCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGc < 1:3376487/61‑1 (MQ=255) tCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:1157327/1‑71 (MQ=255) cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCAc > 1:2013210/1‑52 (MQ=255) cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:569561/1‑70 (MQ=255) cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:2479848/1‑70 (MQ=255) cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:278205/1‑70 (MQ=255) cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:3234757/1‑70 (MQ=255) gATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATc < 1:2291553/70‑1 (MQ=255) cACGAAGTTCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTcaa > 1:744713/1‑68 (MQ=255) cACGAAGTTCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTcaa > 1:2506532/1‑68 (MQ=255) gAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAtt < 1:755867/71‑1 (MQ=255) aaGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAttt > 1:1365511/1‑71 (MQ=255) gCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGt < 1:1182386/71‑1 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa < 1:1728455/50‑1 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa < 1:3657041/50‑1 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa < 1:1527104/50‑1 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc > 1:3173834/1‑71 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc < 1:1632713/71‑1 (MQ=255) aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc > 1:3465633/1‑71 (MQ=255) aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg < 1:1806766/71‑1 (MQ=255) aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc > 1:1032524/1‑70 (MQ=255) aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc > 1:199203/1‑70 (MQ=255) aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc > 1:1049252/1‑70 (MQ=255) cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt < 1:332136/71‑1 (MQ=38) cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt < 1:2256761/71‑1 (MQ=38) cATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc > 1:2182129/1‑70 (MQ=255) attattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa < 1:523442/71‑1 (MQ=255) ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt < 1:341350/38‑1 (MQ=255) ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt < 1:3151277/38‑1 (MQ=255) ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt < 1:2046856/38‑1 (MQ=255) ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTg < 1:3707460/51‑1 (MQ=255) ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat < 1:961065/71‑1 (MQ=255) ttattGAGAACTAGCGCCACGTTATGCTGGTTGTCGAt < 1:1593894/38‑1 (MQ=255) tattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt > 1:2605036/1‑37 (MQ=255) | AGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAAT > W3110S.gb/4080328‑4080465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |