Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,080,432 T→C 8.5% D207D (GAT→GAC glpR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,080,4320TC 8.5% 215.2 / 9.6 82D207D (GAT→GACglpRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (34/41);  new base C (5/2);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

TCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACC  >  W3110S.gb/4080362‑4080501
                                                                      |                                                                     
tCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:1157327/1‑71 (MQ=255)
 cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:2479848/1‑70 (MQ=255)
 cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:569561/1‑70 (MQ=255)
 cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:278205/1‑70 (MQ=255)
 cGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:3234757/1‑70 (MQ=255)
  gATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATc                                                                      <  1:2291553/70‑1 (MQ=255)
        cACGAAGTTCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTcaa                                                                >  1:744713/1‑68 (MQ=255)
        cACGAAGTTCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTcaa                                                                >  1:2506532/1‑68 (MQ=255)
           gAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAtt                                                            <  1:755867/71‑1 (MQ=255)
            aaGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAttt                                                           >  1:1365511/1‑71 (MQ=255)
                  gCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGt                                                     <  1:1182386/71‑1 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa                                                                     <  1:1527104/50‑1 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa                                                                     <  1:3657041/50‑1 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa                                                                     <  1:1728455/50‑1 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                >  1:3465633/1‑71 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                >  1:3173834/1‑71 (MQ=255)
                       aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                <  1:1632713/71‑1 (MQ=255)
                        aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                               <  1:1806766/71‑1 (MQ=255)
                        aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                >  1:199203/1‑70 (MQ=255)
                        aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                >  1:1032524/1‑70 (MQ=255)
                        aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                >  1:1049252/1‑70 (MQ=255)
                          cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                             <  1:2256761/71‑1 (MQ=38)
                          cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                             <  1:332136/71‑1 (MQ=38)
                               cATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                         >  1:2182129/1‑70 (MQ=255)
                                attattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                       <  1:523442/71‑1 (MQ=255)
                                 ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       <  1:2046856/38‑1 (MQ=255)
                                 ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       <  1:3151277/38‑1 (MQ=255)
                                 ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       <  1:341350/38‑1 (MQ=255)
                                 ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTg                                                          <  1:3707460/51‑1 (MQ=255)
                                 ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                      <  1:961065/71‑1 (MQ=255)
                                 ttattGAGAACTAGCGCCACGTTATGCTGGTTGTCGAt                                                                       <  1:1593894/38‑1 (MQ=255)
                                  tattGAGAACTCGCGCCACGTTATGCTGGTTGTCGAt                                                                       >  1:2605036/1‑37 (MQ=255)
                                   attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCa                                                                     >  1:3101564/1‑38 (MQ=255)
                                   attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                         >  1:3719272/1‑66 (MQ=255)
                                   attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAatat                                    >  1:3491836/1‑71 (MQ=255)
                                    ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                              <  1:2682120/44‑1 (MQ=255)
                                    ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                              <  1:215970/44‑1 (MQ=255)
                                    ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                              <  1:3360155/44‑1 (MQ=255)
                                      gagaACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                              <  1:2097693/42‑1 (MQ=255)
                                       agaACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATggg                                 <  1:53472/70‑1 (MQ=255)
                                         aaCTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                      <  1:2149949/63‑1 (MQ=255)
                                           cTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAATTTTg                                                          <  1:3680402/41‑1 (MQ=255)
                                           cTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGa                                              >  1:1951194/1‑53 (MQ=255)
                                            tCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                       >  1:2076936/1‑59 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                   >  1:1826052/1‑46 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                   >  1:3551047/1‑46 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                   >  1:953221/1‑46 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                     >  1:2803816/1‑60 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                           >  1:2042738/1‑70 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                           >  1:2778800/1‑70 (MQ=255)
                                             cgcgCCACGTTATGCTGGTTGTCGATAACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                           >  1:1577774/1‑70 (MQ=255)
                                              gcgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                           >  1:2212447/1‑69 (MQ=255)
                                               cgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                        <  1:2819758/71‑1 (MQ=255)
                                                gcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                        >  1:3229716/1‑70 (MQ=255)
                                                    cGTTATGCTGGTTGTCGATCACTCGAAATTTGTCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                   <  1:1346656/71‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                     <  1:1605821/51‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                     <  1:2448146/51‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcat                        <  1:1933446/64‑2 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                        <  1:1792963/64‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                        <  1:12512/64‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                        <  1:2341648/64‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 <  1:475265/71‑1 (MQ=255)
                                                      ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 <  1:470747/71‑1 (MQ=255)
                                                      ttATGCTGGGTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 <  1:306471/71‑1 (MQ=255)
                                                       tATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 <  1:190684/70‑1 (MQ=255)
                                                        aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                   >  1:45713/1‑67 (MQ=255)
                                                        aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                   >  1:2643268/1‑67 (MQ=255)
                                                          gCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGAGATGGTCAATATGGGcagcatcag                        <  1:3650015/60‑1 (MQ=255)
                                                            tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                               <  1:2041585/35‑1 (MQ=255)
                                                            tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                               <  1:1305118/35‑1 (MQ=255)
                                                             ggTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCg          >  1:2531192/1‑71 (MQ=255)
                                                               ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                            <  1:3385675/51‑1 (MQ=255)
                                                               ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                            <  1:1726367/51‑1 (MQ=255)
                                                               ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc        >  1:2639382/1‑71 (MQ=255)
                                                               ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc        >  1:2596925/1‑71 (MQ=255)
                                                               ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc        <  1:1994186/71‑1 (MQ=255)
                                                                tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                     >  1:1995795/1‑57 (MQ=255)
                                                                tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                     >  1:1514206/1‑57 (MQ=255)
                                                                tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                     >  1:2777158/1‑57 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                     >  1:357783/1‑40 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 >  1:3302244/1‑60 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 >  1:1787557/1‑60 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa               >  1:2409305/1‑62 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa               >  1:1859399/1‑62 (MQ=255)
                                                                 gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa               >  1:869334/1‑62 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                 <  1:881355/59‑1 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAg                >  1:259925/1‑60 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa               >  1:2562805/1‑61 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac     <  1:3368839/71‑1 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac     <  1:1819831/71‑1 (MQ=255)
                                                                  tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTa      <  1:3119924/70‑1 (MQ=255)
                                                                     aTCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACAcc  <  1:1320844/71‑1 (MQ=255)
                                                                      |                                                                     
TCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACC  >  W3110S.gb/4080362‑4080501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: