Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,080,438 G→A 5.9% S209S (TCG→TCA glpR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,080,4380GA 5.9% 259.6 / 3.9 84S209S (TCG→TCAglpRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (35/44);  new base A (3/2);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCC  >  W3110S.gb/4080373‑4080508
                                                                 |                                                                      
gAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAtt                                                                   <  1:755867/71‑1 (MQ=255)
 aaGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAAttt                                                                  >  1:1365511/1‑71 (MQ=255)
       gCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGt                                                            <  1:1182386/71‑1 (MQ=255)
            aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       >  1:3173834/1‑71 (MQ=255)
            aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       <  1:1632713/71‑1 (MQ=255)
            aaaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       >  1:3465633/1‑71 (MQ=255)
             aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                      <  1:1806766/71‑1 (MQ=255)
             aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       >  1:199203/1‑70 (MQ=255)
             aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       >  1:1049252/1‑70 (MQ=255)
             aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgc                                                       >  1:1032524/1‑70 (MQ=255)
               cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                                    <  1:332136/71‑1 (MQ=38)
               cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                                    <  1:2256761/71‑1 (MQ=38)
                    cATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                                >  1:2182129/1‑70 (MQ=255)
                     attattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                              <  1:523442/71‑1 (MQ=255)
                      ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTg                                                                 <  1:3707460/51‑1 (MQ=255)
                      ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                             <  1:961065/71‑1 (MQ=255)
                        attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                                >  1:3719272/1‑66 (MQ=255)
                        attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAatat                                           >  1:3491836/1‑71 (MQ=255)
                         ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                                     <  1:215970/44‑1 (MQ=255)
                         ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                                     <  1:3360155/44‑1 (MQ=255)
                         ttGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                                     <  1:2682120/44‑1 (MQ=255)
                           gagaACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGaaa                                                                     <  1:2097693/42‑1 (MQ=255)
                            agaACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATggg                                        <  1:53472/70‑1 (MQ=255)
                              aaCTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                             <  1:2149949/63‑1 (MQ=255)
                                cTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAATTTTg                                                                 <  1:3680402/41‑1 (MQ=255)
                                cTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGa                                                     >  1:1951194/1‑53 (MQ=255)
                                 tCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                              >  1:2076936/1‑59 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                          >  1:1826052/1‑46 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                          >  1:3551047/1‑46 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTaa                                                          >  1:953221/1‑46 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                            >  1:2803816/1‑60 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                  >  1:2778800/1‑70 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                  >  1:2042738/1‑70 (MQ=255)
                                  cgcgCCACGTTATGCTGGTTGTCGATAACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                  >  1:1577774/1‑70 (MQ=255)
                                   gcgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                  >  1:2212447/1‑69 (MQ=255)
                                    cgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               <  1:2819758/71‑1 (MQ=255)
                                     gcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               >  1:3229716/1‑70 (MQ=255)
                                         cGTTATGCTGGTTGTCGATCACTCGAAATTTGTCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                          <  1:1346656/71‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                            <  1:2448146/51‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                            <  1:1605821/51‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcat                               <  1:1933446/64‑2 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               <  1:2341648/64‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               <  1:12512/64‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               <  1:1792963/64‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:475265/71‑1 (MQ=255)
                                           ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:470747/71‑1 (MQ=255)
                                           ttATGCTGGGTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:306471/71‑1 (MQ=255)
                                            tATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:190684/70‑1 (MQ=255)
                                             aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                          >  1:45713/1‑67 (MQ=255)
                                             aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                          >  1:2643268/1‑67 (MQ=255)
                                               gCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGAGATGGTCAATATGGGcagcatcag                               <  1:3650015/60‑1 (MQ=255)
                                                 tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                      <  1:2041585/35‑1 (MQ=255)
                                                 tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                      <  1:1305118/35‑1 (MQ=255)
                                                  ggTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCg                 >  1:2531192/1‑71 (MQ=255)
                                                    ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                                   <  1:3385675/51‑1 (MQ=255)
                                                    ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                                   <  1:1726367/51‑1 (MQ=255)
                                                    ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               >  1:2639382/1‑71 (MQ=255)
                                                    ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               <  1:1994186/71‑1 (MQ=255)
                                                    ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               >  1:2596925/1‑71 (MQ=255)
                                                     tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                            >  1:2777158/1‑57 (MQ=255)
                                                     tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                            >  1:1514206/1‑57 (MQ=255)
                                                     tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                            >  1:1995795/1‑57 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                            >  1:357783/1‑40 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        >  1:1787557/1‑60 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        >  1:3302244/1‑60 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                      >  1:869334/1‑62 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                      >  1:1859399/1‑62 (MQ=255)
                                                      gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                      >  1:2409305/1‑62 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:881355/59‑1 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAg                       >  1:259925/1‑60 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                      >  1:2562805/1‑61 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac            <  1:1819831/71‑1 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac            <  1:3368839/71‑1 (MQ=255)
                                                       tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTa             <  1:3119924/70‑1 (MQ=255)
                                                          aTCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACAcc         <  1:1320844/71‑1 (MQ=255)
                                                              cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               <  1:2984161/61‑1 (MQ=255)
                                                              cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               <  1:1953566/61‑1 (MQ=255)
                                                              cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc               <  1:1729965/61‑1 (MQ=255)
                                                               tCGAAATTTGGCCGTAACGCGATGGTTAATATGGGCAGCATCAGCATGGTa                        <  1:946207/51‑1 (MQ=255)
                                                               tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                               <  1:1009051/44‑1 (MQ=255)
                                                               tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:2519491/51‑1 (MQ=255)
                                                               tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:1552687/51‑1 (MQ=255)
                                                               tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGc    >  1:1436559/1‑71 (MQ=255)
                                                                cGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatc                                 >  1:173280/1‑41 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATTGTCAATATGGGCAGCATCAGCATGCTa                        <  1:1775403/49‑1 (MQ=38)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGgca                                      >  1:931199/1‑35 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGgca                                      >  1:558945/1‑35 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcagcattg                          >  1:1090942/1‑45 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                          >  1:517201/1‑47 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:3431413/49‑1 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                        <  1:1986197/49‑1 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTaca           >  1:2779161/1‑62 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGCCGccc  >  1:1355083/1‑71 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc  >  1:1797234/1‑71 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc  >  1:2350724/1‑71 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc  >  1:951533/1‑71 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc  >  1:391586/1‑71 (MQ=255)
                                                                 gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc  >  1:997143/1‑71 (MQ=255)
                                                                 |                                                                      
GAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCC  >  W3110S.gb/4080373‑4080508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: