Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,080,456 G→A 6.1% A215A (GCG→GCA glpR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,080,4560GA 6.1% 247.1 / 3.9 81A215A (GCG→GCAglpRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base A (3/2);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

AACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGT  >  W3110S.gb/4080386‑4080524
                                                                      |                                                                    
aaCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                                      <  1:1806766/71‑1 (MQ=255)
  cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                                                    <  1:2256761/71‑1 (MQ=38)
  cgcgCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTCAAAATTTGGCCGTAACGCAAt                                                                    <  1:332136/71‑1 (MQ=38)
       cATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                                                >  1:2182129/1‑70 (MQ=255)
        attattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                                              <  1:523442/71‑1 (MQ=255)
         ttattGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                                             <  1:961065/71‑1 (MQ=255)
           attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTc                                                                >  1:3719272/1‑66 (MQ=255)
           attGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAatat                                                           >  1:3491836/1‑71 (MQ=255)
               agaACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATggg                                                        <  1:53472/70‑1 (MQ=255)
                 aaCTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAat                                                             <  1:2149949/63‑1 (MQ=255)
                   cTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGa                                                                     >  1:1951194/1‑53 (MQ=255)
                    tCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCaa                                                              >  1:2076936/1‑59 (MQ=255)
                     cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                                            >  1:2803816/1‑60 (MQ=255)
                     cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                                  >  1:2042738/1‑70 (MQ=255)
                     cgcgCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                                  >  1:2778800/1‑70 (MQ=255)
                     cgcgCCACGTTATGCTGGTTGTCGATAACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                                  >  1:1577774/1‑70 (MQ=255)
                      gcgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcat                                                  >  1:2212447/1‑69 (MQ=255)
                       cgcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               <  1:2819758/71‑1 (MQ=255)
                        gcCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               >  1:3229716/1‑70 (MQ=255)
                            cGTTATGCTGGTTGTCGATCACTCGAAATTTGTCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                                          <  1:1346656/71‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                                            <  1:1605821/51‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                                            <  1:2448146/51‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcat                                               <  1:1933446/64‑2 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               <  1:1792963/64‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               <  1:12512/64‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               <  1:2341648/64‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:470747/71‑1 (MQ=255)
                              ttATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:475265/71‑1 (MQ=255)
                              ttATGCTGGGTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:306471/71‑1 (MQ=255)
                               tATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:190684/70‑1 (MQ=255)
                                aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                                          >  1:2643268/1‑67 (MQ=255)
                                aTGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                                          >  1:45713/1‑67 (MQ=255)
                                  gCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGAGATGGTCAATATGGGcagcatcag                                               <  1:3650015/60‑1 (MQ=255)
                                    tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                                      <  1:1305118/35‑1 (MQ=255)
                                    tGGTTGTCGATCACTCGAAATTTGGCCGTAAcgcg                                                                      <  1:2041585/35‑1 (MQ=255)
                                     ggTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCg                                 >  1:2531192/1‑71 (MQ=255)
                                       ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                                                   <  1:3385675/51‑1 (MQ=255)
                                       ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGgcagca                                                   <  1:1726367/51‑1 (MQ=255)
                                       ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               <  1:1994186/71‑1 (MQ=255)
                                       ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               >  1:2639382/1‑71 (MQ=255)
                                       ttGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               >  1:2596925/1‑71 (MQ=255)
                                        tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                                            >  1:1995795/1‑57 (MQ=255)
                                        tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                                            >  1:1514206/1‑57 (MQ=255)
                                        tGTCGACCACTCAAAATTTGGCCGTAACGCAATGGTCAATATGGGtagcatcagcat                                            >  1:2777158/1‑57 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAata                                                            >  1:357783/1‑40 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        >  1:1787557/1‑60 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        >  1:3302244/1‑60 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                                      >  1:1859399/1‑62 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                                      >  1:2409305/1‑62 (MQ=255)
                                         gTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                                      >  1:869334/1‑62 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:881355/59‑1 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAg                                       >  1:259925/1‑60 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGa                                      >  1:2562805/1‑61 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac                            <  1:1819831/71‑1 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTac                            <  1:3368839/71‑1 (MQ=255)
                                          tCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTa                             <  1:3119924/70‑1 (MQ=255)
                                             aTCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACAcc                         <  1:1320844/71‑1 (MQ=255)
                                                 cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               <  1:1729965/61‑1 (MQ=255)
                                                 cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               <  1:2984161/61‑1 (MQ=255)
                                                 cTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTc                               <  1:1953566/61‑1 (MQ=255)
                                                  tCGAAATTTGGCCGTAACGCGATGGTTAATATGGGCAGCATCAGCATGGTa                                        <  1:946207/51‑1 (MQ=255)
                                                  tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcag                                               <  1:1009051/44‑1 (MQ=255)
                                                  tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:1552687/51‑1 (MQ=255)
                                                  tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:2519491/51‑1 (MQ=255)
                                                  tCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGc                    >  1:1436559/1‑71 (MQ=255)
                                                   cGAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatc                                                 >  1:173280/1‑41 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATTGTCAATATGGGCAGCATCAGCATGCTa                                        <  1:1775403/49‑1 (MQ=38)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGgca                                                      >  1:558945/1‑35 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGgca                                                      >  1:931199/1‑35 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGcagcatcagcattg                                          >  1:1090942/1‑45 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATgg                                          >  1:517201/1‑47 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:3431413/49‑1 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        <  1:1986197/49‑1 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTaca                           >  1:2779161/1‑62 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGCCGccc                  >  1:1355083/1‑71 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc                  >  1:997143/1‑71 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc                  >  1:951533/1‑71 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc                  >  1:1797234/1‑71 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc                  >  1:2350724/1‑71 (MQ=255)
                                                    gAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGccc                  >  1:391586/1‑71 (MQ=255)
                                                      aaTTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCccg                <  1:3450315/71‑1 (MQ=255)
                                                              cGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTa                                        >  1:2972549/1‑39 (MQ=255)
                                                                  aCGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGc    >  1:2503159/1‑71 (MQ=255)
                                                                  aCGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGc    >  1:2856437/1‑71 (MQ=255)
                                                                   cgcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCACGCCCCGCCGCCAGTAAGCGt  <  1:2674315/71‑1 (MQ=255)
                                                                    gcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCTGTAAGCGt  <  1:2732754/71‑1 (MQ=255)
                                                                    gcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGt  <  1:2391885/71‑1 (MQ=255)
                                                                    gcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGt  <  1:2225152/71‑1 (MQ=255)
                                                                    gcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGt  <  1:2937666/71‑1 (MQ=255)
                                                                    gcgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGt  <  1:1705444/71‑1 (MQ=255)
                                                                     cgATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGt  <  1:3365862/70‑1 (MQ=255)
                                                                      |                                                                    
AACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTAACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGT  >  W3110S.gb/4080386‑4080524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: