| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,095,396 | G→T | 16.1% | S47S (TCC→TCA) | gntX ← | gluconate periplasmic binding protein with phosphoribosyltransferase domain, GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,095,396 | 0 | G | T | 16.1% | 83.4 / 3.7 | 31 | S47S (TCC→TCA) | gntX | gluconate periplasmic binding protein with phosphoribosyltransferase domain, GNT I system |
| Reads supporting (aligned to +/- strand): ref base G (6/20); new base T (2/3); total (8/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
ATAGTCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGAACAGACCG > W3110S.gb/4095327‑4095466 | aTAGTCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATggg < 1:910968/71‑1 (MQ=255) gTCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAg < 1:1546823/70‑1 (MQ=255) gTCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAg < 1:1958304/70‑1 (MQ=255) tCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAgtg < 1:783589/71‑1 (MQ=255) gCAACC‑TGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGtgtgtg < 1:1252874/71‑1 (MQ=255) tGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGt < 1:2511572/71‑1 (MQ=255) tGCAAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGTAGTGTGTGGCGGGTAATCCACATtg > 1:2916233/1‑71 (MQ=255) ccAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAgtg < 1:3668399/48‑1 (MQ=255) ccAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAgtg < 1:2421176/48‑1 (MQ=255) ccAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAgtg < 1:2319221/48‑1 (MQ=255) ccAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATtgt < 1:694049/70‑1 (MQ=255) ggcggcGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACAt < 1:3105945/63‑1 (MQ=255) gcggcgGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCAt < 1:576566/71‑1 (MQ=255) gcggTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAAc < 1:234120/71‑1 (MQ=255) gcggTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAAc < 1:2040024/71‑1 (MQ=255) ggTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGt < 1:189768/71‑1 (MQ=255) gTTTTTGCAGGCAGCGACCGCAGGGAAGATGTGAGTGTGTGGCGGGTAATCCACATtgg > 1:3120379/1‑58 (MQ=255) gTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAgtg < 1:3501705/37‑1 (MQ=255) tttGCAGGCAGCGACCGCAGGGAAGATGTGAGTGTGTGGCGGGTAATCCACATtggg < 1:29703/57‑3 (MQ=255) tttGCAGGCAGCGACCGCAGGGAAGATGTGAGTGTGTGGCGGGTAATCCACATtggg < 1:1461307/57‑3 (MQ=255) tttGCAGGCAGCGACAGCAGGGAAGATGTGAGTGTGTGGCGGGTAATCCACATtggg < 1:372461/57‑3 (MQ=255) agCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAAc > 1:1642275/1‑56 (MQ=255) aCCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTgg > 1:3017985/1‑45 (MQ=255) ggAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACg < 1:3260201/46‑1 (MQ=255) gAAGATGTGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCg > 1:3386054/1‑58 (MQ=255) gAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACg < 1:561478/45‑1 (MQ=255) gAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCg > 1:1570907/1‑58 (MQ=255) gAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGaa < 1:2854228/71‑1 (MQ=255) aaGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGaa > 1:2939879/1‑70 (MQ=255) aGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGAACa < 1:933267/71‑1 (MQ=255) gATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGTGTGGCGCGTGAACAg < 1:503799/71‑1 (MQ=255) tGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGAACAGAc > 1:3510436/1‑71 (MQ=255) ggAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGAACAGACCg < 1:529972/71‑1 (MQ=255) | ATAGTCGGCAACCGTGACCAGTCTTTGCCAGGGCGGCGGTTTTTGCAGGCAGCGACCGCAGGGAAGATGGGAGTGTGTGGCGGGTAATCCACATTGTGGGCATAACGTTTTATCTGTGCGGGTGGCGCGTGAACAGACCG > W3110S.gb/4095327‑4095466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |