| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 383,415 | G→A | 12.9% | C142C (TGC→TGT) | yaiS ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 383,415 | 0 | G | A | 12.9% | 158.3 / 12.4 | 62 | C142C (TGC→TGT) | yaiS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (31/23); new base A (6/2); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
CAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATA > W3110S.gb/383345‑383483 | cAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg > 1:3464351/1‑71 (MQ=255) aGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAAc < 1:3411949/69‑1 (MQ=255) ggCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCAt < 1:2573223/71‑1 (MQ=255) aTAAATGAAAGCCAGGTACTTGGTGTTTCATAGCCGAGAATTTGCGGAATAGCGCGACAGGCAACCATTg < 1:2281325/70‑1 (MQ=255) aTAAATGAAAGCCAGGTACTTGGTGTTTCATAGCCGAGAATTTGCGGAATAGCGCGACAGGCAACCATTg < 1:1660928/70‑1 (MQ=255) aaTGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAg < 1:430443/70‑1 (MQ=255) aaTGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGc > 1:3858162/1‑71 (MQ=255) aTGAAAGCCAGTTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg < 1:3200639/53‑1 (MQ=255) aTGAAAGCCAGTTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg < 1:3204888/53‑1 (MQ=255) aTGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCt > 1:866639/1‑71 (MQ=255) tGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCtt > 1:467061/1‑71 (MQ=255) aaaGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGa > 1:2119809/1‑71 (MQ=255) aaaGCCAGGTACTTGGGGTTACGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGa > 1:406274/1‑71 (MQ=255) gCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATaa < 1:1567536/71‑1 (MQ=255) cAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATaaa > 1:1914026/1‑70 (MQ=255) gTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAAc < 1:1057752/50‑1 (MQ=255) gTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGc < 1:1504790/71‑1 (MQ=255) gTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGc < 1:2276275/71‑1 (MQ=255) aCTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCa > 1:1834589/1‑50 (MQ=255) aCTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCa > 1:496236/1‑50 (MQ=255) aCTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGc > 1:3455924/1‑69 (MQ=255) aCTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGc > 1:3451364/1‑69 (MQ=255) cTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAg < 1:2521801/71‑1 (MQ=255) tGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:2279054/1‑71 (MQ=255) gggTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga > 1:75734/1‑71 (MQ=255) gggTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:1477701/71‑1 (MQ=255) ggTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatgat < 1:3725593/71‑1 (MQ=255) gTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTg < 1:2755002/46‑1 (MQ=255) gTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTg < 1:1679275/46‑1 (MQ=255) tttCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATa < 1:1687333/55‑1 (MQ=255) ttCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCt > 1:1295420/1‑71 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCATTGATGCCTGATAAACAGCaat > 1:2276920/1‑61 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCATTGATGCCTGATAAACAGCaat > 1:3504251/1‑61 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCATTGATGCCTGATAAACAGCaat > 1:2043219/1‑61 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCATTGATGCCTGATAAACAGCaat > 1:3323956/1‑61 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCATTGATGCCTGATAAACAGCaat > 1:763707/1‑61 (MQ=255) tCGTATCCTAGTATTTGAGGAATATAACGACAGGCAACCACTGATGCCTGATAAACAGCaat > 1:253518/1‑61 (MQ=255) tCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATc < 1:253878/68‑1 (MQ=255) tCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCt > 1:526839/1‑70 (MQ=255) tCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCt > 1:2238119/1‑70 (MQ=255) tCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCt > 1:3561548/1‑70 (MQ=255) gCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCa > 1:3024010/1‑35 (MQ=255) agaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTc > 1:1518414/1‑71 (MQ=255) gaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCg > 1:724604/1‑71 (MQ=255) gaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCg > 1:2299508/1‑71 (MQ=255) gaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCg > 1:1691189/1‑71 (MQ=255) tttGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:1193483/1‑51 (MQ=255) tttGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:1580036/1‑51 (MQ=255) tttGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:3851630/1‑51 (MQ=255) tttGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:2338525/1‑51 (MQ=255) tttGCGGAATAGTTAGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgat > 1:3718535/1‑51 (MQ=255) tGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTg > 1:2275670/1‑56 (MQ=255) tGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATg > 1:2691859/1‑59 (MQ=255) gCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGcatc < 1:1320471/70‑1 (MQ=255) gCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGGCGGcatc < 1:2718759/70‑1 (MQ=255) ggAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCgg < 1:342730/64‑1 (MQ=255) ggAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCgg < 1:1071525/64‑1 (MQ=255) aaTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATcc > 1:3416899/1‑49 (MQ=255) aaTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGa > 1:1331827/1‑52 (MQ=255) ttCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:2454484/41‑1 (MQ=255) ttCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:1431011/41‑1 (MQ=255) ttCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:444096/41‑1 (MQ=255) ttCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:2587517/41‑1 (MQ=255) ttCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAgatga < 1:1177854/41‑1 (MQ=255) cGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGtata > 1:283763/1‑71 (MQ=255) | CAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATA > W3110S.gb/383345‑383483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |