| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,110,393 | A→G | 8.2% | N84N (AAT→AAC) | hslO ← | heat shock protein Hsp33 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,110,393 | 0 | A | G | 8.2% | 159.7 / 5.3 | 61 | N84N (AAT→AAC) | hslO | heat shock protein Hsp33 |
| Reads supporting (aligned to +/- strand): ref base A (26/30); new base G (3/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CCAGCGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGATATCACCATC > W3110S.gb/4110323‑4110461 | ccAGCGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTa < 1:291441/71‑1 (MQ=255) gCGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCAGGCGCGCCACACCGCGCATCTGCTGGTTATTg > 1:3183848/1‑71 (MQ=255) cGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGt < 1:2603007/71‑1 (MQ=255) cGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGt < 1:3423771/71‑1 (MQ=255) tCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCg > 1:3742450/1‑71 (MQ=255) cAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGt > 1:971602/1‑71 (MQ=255) ggTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTta < 1:3549897/71‑1 (MQ=255) tCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTtaat < 1:2103188/71‑1 (MQ=255) ggCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGt < 1:420919/59‑1 (MQ=255) ggCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGt < 1:673281/59‑1 (MQ=255) gCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAAc < 1:3172567/71‑1 (MQ=255) ttttCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGt < 1:1908721/55‑1 (MQ=255) tCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTGTTGTTACCGTTAATAACCGCCa < 1:2465860/70‑1 (MQ=255) tCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTGTTGTTACCGTTAATAACCGCCa < 1:2223475/70‑1 (MQ=255) cTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTAc > 1:1234037/1‑54 (MQ=255) tGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTg > 1:1674008/1‑49 (MQ=255) gAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGAtt < 1:1256376/70‑1 (MQ=255) gAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGAtt > 1:2598377/1‑70 (MQ=255) gAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTc < 1:194016/71‑1 (MQ=255) aTTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCAt < 1:1738090/71‑1 (MQ=255) tttCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCa < 1:392242/63‑1 (MQ=255) tCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCg > 1:2416808/1‑70 (MQ=255) tCGCCCTGCACGCGCGCCACACCGCGCATCTGCTCGTTATTGTTACCGTTAATAACCGCCAGATTCATCg > 1:491604/1‑70 (MQ=255) cGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCgg < 1:3187614/70‑1 (MQ=255) cGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCgg < 1:3593501/70‑1 (MQ=255) tGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCa < 1:67685/55‑1 (MQ=255) tGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTc > 1:3756651/1‑71 (MQ=255) gCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTc > 1:3172146/1‑70 (MQ=255) cACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCgg > 1:3354273/1‑63 (MQ=255) cACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGc > 1:3194326/1‑71 (MQ=255) cACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGc > 1:2341562/1‑71 (MQ=255) cgcgcCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTg < 1:1210474/71‑1 (MQ=255) gcCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAg > 1:3749010/1‑71 (MQ=255) cacaCCGCGCATCTGCTGGTTATTGTTACCGTtaat < 1:2555523/36‑1 (MQ=255) acacCGCGCATCTGCTGGTTATTGTTACCGTTAATAAcc > 1:3224451/1‑39 (MQ=255) acacCGCGCATCTGCTGGTTATTGTTACCGTTAATAAcc > 1:1425700/1‑39 (MQ=255) acacCGCGCATCTGCTGGTTATTGTTACCGTTAATAAcc > 1:1619994/1‑39 (MQ=255) ccGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCt < 1:1507799/61‑1 (MQ=255) cgcATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:2251674/71‑1 (MQ=255) cgcATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:3348794/71‑1 (MQ=255) gcATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTg < 1:3207979/71‑1 (MQ=255) tCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGAcc < 1:678208/46‑1 (MQ=255) tgctgGTTGTTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCTTGCAGCTGTACGGTGatat > 1:524141/1‑70 (MQ=255) tgctgGTTGTTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCTTGCAGCTGTACGGTGatat > 1:3784802/1‑70 (MQ=255) tgctgGTTGTTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCTTGCAGCTGTACGGTGat > 1:83205/1‑68 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCAt > 1:691275/1‑38 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:2875273/1‑70 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:1627629/1‑70 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:1589868/1‑70 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:594360/1‑70 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:3281347/1‑70 (MQ=255) tgctgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:105678/1‑70 (MQ=255) tgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:541547/62‑1 (MQ=255) tgGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:58522/62‑1 (MQ=255) gTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTa < 1:3534170/56‑1 (MQ=255) gTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTa < 1:2199067/56‑1 (MQ=255) ttATTGTTACCGTTAATAACCGCCAGATTCATCgg < 1:1709877/35‑1 (MQ=255) ttATTGTTACCGTTAATAACCGCCAGATTCATCgg < 1:3517045/35‑1 (MQ=255) ttATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:1849868/59‑1 (MQ=255) ttATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGt < 1:1239895/59‑1 (MQ=255) tATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGATATCACCATc > 1:1840409/1‑70 (MQ=255) tATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGATATCACCATc > 1:2755970/1‑70 (MQ=255) aTTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGatat > 1:2922042/1‑62 (MQ=255) | CCAGCGTTTTCAGGTCGGCATTTTCTGGAATTTCGCCCTGCACGCGCGCCACACCGCGCATCTGCTGGTTATTGTTACCGTTAATAACCGCCAGATTCATCGGACCGTCGCCCTGCAGCTGTACGGTGATATCACCATC > W3110S.gb/4110323‑4110461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |