| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,217,050 | A→G | 11.1% | A75A (GCA→GCG) | yjaA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,217,050 | 0 | A | G | 11.1% | 155.6 / 10.5 | 63 | A75A (GCA→GCG) | yjaA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (37/19); new base G (2/5); total (39/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
CCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTG > W3110S.gb/4216987‑4217114 | ccacccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGGGCACTGGaaa > 1:1518844/1‑71 (MQ=255)ccacccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaaa > 1:1032229/1‑71 (MQ=255)ccacccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaa > 1:2309265/1‑70 (MQ=255) acccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatg < 1:107489/71‑1 (MQ=255) cccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatga < 1:3115539/71‑1 (MQ=255) cccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatga < 1:2066797/71‑1 (MQ=255) ccacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaa < 1:1537737/71‑1 (MQ=255) cacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCa > 1:2896727/1‑59 (MQ=255) cacGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaat > 1:1908281/1‑71 (MQ=255) acGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaaa > 1:760468/1‑65 (MQ=255) acGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaaa > 1:2975031/1‑65 (MQ=255) aCCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGa < 1:2279372/56‑1 (MQ=255) tGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaaa > 1:1542589/1‑55 (MQ=255) tGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaatg < 1:3789326/61‑1 (MQ=255) tGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaa > 1:2450974/1‑59 (MQ=255) ggCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaa > 1:2484536/1‑58 (MQ=255) gCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaat > 1:2655252/1‑58 (MQ=255) gCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGt < 1:2631288/71‑1 (MQ=255) aTTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGttt < 1:3503385/67‑1 (MQ=255) ttCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaa < 1:1568556/49‑1 (MQ=255) ttCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGaa < 1:1161490/49‑1 (MQ=255) ttCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaat < 1:1838401/55‑1 (MQ=255) ttCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTca > 1:3150493/1‑70 (MQ=255) ttCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTc > 1:1110452/1‑69 (MQ=255) tCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGt < 1:3112942/67‑1 (MQ=255) ttttttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTcaca > 1:665957/1‑69 (MQ=255) ttttttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTcaca > 1:1977235/1‑69 (MQ=255) ttttttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTcaca > 1:656640/1‑69 (MQ=255) ttttttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAgg > 1:2931679/1‑71 (MQ=255) ttttttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAgg > 1:160897/1‑71 (MQ=255) tttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGtt > 1:863669/1‑70 (MQ=255) tttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGtt > 1:856998/1‑70 (MQ=255) tttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGtt > 1:2336960/1‑70 (MQ=255) tttGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGtt > 1:224665/1‑70 (MQ=255) tGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGa < 1:2365978/70‑1 (MQ=255) gCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAg < 1:1089286/70‑1 (MQ=255) ccAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAgg < 1:1151174/70‑1 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACg > 1:3365963/1‑71 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACg > 1:1330018/1‑71 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaatgaa > 1:2264166/1‑41 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaat < 1:3712361/38‑1 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCACTGGAaat < 1:1061632/38‑1 (MQ=255) gCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACg < 1:487468/71‑1 (MQ=255) cAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACg > 1:2393539/1‑70 (MQ=255) aaaaCGTATGGATATTGTTGTGAGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCa < 1:1071180/71‑1 (MQ=255) aaaaCGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGt > 1:439002/1‑53 (MQ=255) aaaCGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAg > 1:2647741/1‑42 (MQ=255) aCGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATtc > 1:984507/1‑71 (MQ=255) gTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATtctc > 1:1697872/1‑71 (MQ=255) gATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAtt > 1:457999/1‑63 (MQ=255) gATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATg > 1:3425991/1‑70 (MQ=255) gATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATg > 1:2289786/1‑70 (MQ=255) atTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGtt < 1:291468/48‑1 (MQ=255) atTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTGTCCATGaa > 1:1398015/1‑69 (MQ=255) tgttgtGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGTACGCAt > 1:2595232/1‑57 (MQ=255) tgttgtGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTgg > 1:961702/1‑71 (MQ=255) gttgtGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGa > 1:2670213/1‑47 (MQ=255) gttgtGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGa > 1:1459744/1‑47 (MQ=255) gttgtGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGaa > 1:466015/1‑51 (MQ=255) gtgAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGc > 1:2811549/1‑71 (MQ=255) aGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTTCATGAAGTGGTCGcag < 1:2582986/70‑3 (MQ=255) aGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTTCATGAAGTGGTCGcag < 1:1790848/70‑3 (MQ=255) aGCGCGCTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTTCATGAAGTGGTCGcag < 1:1717435/70‑3 (MQ=255) aGCGCGCTGGAAATGAATGAGGGAGGTTTGTCACAGGTTGAGGAACGCATTCTTCATGAAGTGGTCGcag < 1:2508033/70‑3 (MQ=255) | CCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTG > W3110S.gb/4216987‑4217114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |