| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,258,286 | T→A | 8.8% | T591T (ACA→ACT) | plsB ← | glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,258,286 | 0 | T | A | 8.8% | 149.6 / 6.7 | 57 | T591T (ACA→ACT) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (35/17); new base A (3/2); total (38/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAG > W3110S.gb/4258217‑4258356 | cgcAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCTAtt < 1:3273060/70‑1 (MQ=255) gcAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtgt > 1:2764670/1‑71 (MQ=255) cAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtgtg > 1:1472862/1‑71 (MQ=255) aaTGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtgtg > 1:1233407/1‑70 (MQ=255) aaTGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATtgtg > 1:1327706/1‑70 (MQ=255) aaTGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTgtgt > 1:2279926/1‑71 (MQ=255) aaTGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTgtgt > 1:1884232/1‑71 (MQ=255) tgttgtTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCt < 1:1321541/71‑1 (MQ=255) ttgttgCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCttt > 1:2561773/1‑71 (MQ=255) ttgttgCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCttt < 1:1845441/71‑1 (MQ=255) ttgttgCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCtt > 1:1687133/1‑70 (MQ=255) ttgttgCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCtt > 1:171170/1‑70 (MQ=255) gtttCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCTATTGTGTCTTtc < 1:1955105/70‑1 (MQ=255) gttgCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTtct > 1:1957676/1‑71 (MQ=255) gCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCg < 1:3093492/70‑1 (MQ=255) gCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGa > 1:1744883/1‑71 (MQ=255) aTAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACtt < 1:1364721/71‑1 (MQ=255) gtagGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCa > 1:2032116/1‑70 (MQ=255) gtagGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCa > 1:1223453/1‑70 (MQ=255) ggTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTTGACTTCaaa > 1:809772/1‑69 (MQ=255) ggTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAAc < 1:1592174/70‑1 (MQ=255) gTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTtc < 1:1391928/57‑1 (MQ=255) gTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTtc < 1:2453540/57‑1 (MQ=255) tcaGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACt < 1:3109129/66‑1 (MQ=255) tcaGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACt < 1:263335/66‑1 (MQ=255) caGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCaaa > 1:1858144/1‑63 (MQ=255) caGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCaaa > 1:2757383/1‑63 (MQ=255) caGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCaaa > 1:3043470/1‑63 (MQ=255) agcagcGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTtctc > 1:2806844/1‑53 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCaa > 1:2581852/1‑61 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCAt > 1:1803866/1‑71 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCAt > 1:879725/1‑71 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATAGTGTCTTTCTCAACTTCAAACTTGTTCAt > 1:2727510/1‑71 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATAGTGTCTTTCTCAACTTCAAACTTGTTCAt > 1:2732134/1‑71 (MQ=255) aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATAGTGTCTTTCTCAACTTCAAACTTGTTCAt > 1:1075616/1‑71 (MQ=255) aCCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTg < 1:2061870/71‑1 (MQ=255) aCCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTg < 1:3474423/71‑1 (MQ=255) ccGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTg > 1:1401807/1‑70 (MQ=255) cGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTg > 1:1184066/1‑69 (MQ=255) cGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATAGTGTCTTtctc < 1:3540968/48‑1 (MQ=255) cGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATAGTGTCTTtctc < 1:2894892/48‑1 (MQ=255) gCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAg > 1:2120942/1‑71 (MQ=255) gCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAg < 1:525508/71‑1 (MQ=255) gCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAg > 1:2934137/1‑71 (MQ=255) cTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAgc > 1:1171240/1‑71 (MQ=255) tGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAgcgc > 1:3650985/1‑71 (MQ=255) gcgcgGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTg < 1:2243418/69‑1 (MQ=255) cgGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGa < 1:2816432/71‑1 (MQ=255) cAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATaa < 1:3403249/71‑1 (MQ=255) aGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAg > 1:953906/1‑71 (MQ=255) aGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAg > 1:1719367/1‑71 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCaaa > 1:1334938/1‑38 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCaaa > 1:1180667/1‑38 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAgcg > 1:2441881/1‑55 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTc > 1:2567131/1‑71 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTc > 1:2013196/1‑71 (MQ=255) atgatgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTc > 1:3661496/1‑71 (MQ=255) atgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTcgct < 1:987120/71‑1 (MQ=255) atgatgTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTcgct > 1:3349803/1‑71 (MQ=255) tCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGa > 1:1109941/1‑55 (MQ=255) tCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTcgctggcgct > 1:2447624/1‑71 (MQ=255) gATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCgctggct > 1:2804010/1‑70 (MQ=255) tgtgTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAg > 1:2757910/1‑71 (MQ=255) | CGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAG > W3110S.gb/4258217‑4258356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |