| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,317,126 | G→A | 6.4% | S182S (TCC→TCT) | rpiR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,317,126 | 0 | G | A | 6.4% | 380.4 / 9.3 | 124 | S182S (TCC→TCT) | rpiR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (57/59); new base A (4/4); total (61/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 129 total aligned reads displayed.
TTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTA > W3110S.gb/4317058‑4317196 | tttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGa > 1:3331759/1‑71 (MQ=255) ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCgg < 1:2945111/69‑1 (MQ=255) ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCgg < 1:3861286/69‑1 (MQ=255) ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGa > 1:1722840/1‑70 (MQ=255) aCATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcat > 1:2364003/1‑71 (MQ=255) aTCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatca > 1:3153678/1‑71 (MQ=255) aTCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatca > 1:2689159/1‑71 (MQ=255) aTCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatca > 1:3731712/1‑71 (MQ=255) cACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcat > 1:1205942/1‑70 (MQ=255) cTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat < 1:3448878/71‑1 (MQ=255) ttCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCatgatg < 1:3157732/71‑1 (MQ=255) tCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAc > 1:3097423/1‑58 (MQ=255) cGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgt < 1:1686054/70‑1 (MQ=255) cGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgt < 1:1889645/70‑1 (MQ=255) cGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgt < 1:3802810/70‑1 (MQ=255) cccGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAg < 1:106659/71‑1 (MQ=255) ggAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCAt > 1:3209109/1‑71 (MQ=255) gAATCGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGTAGCGGACATCATCATGATGTGAGCAt > 1:2685306/1‑70 (MQ=255) aaTGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCAt > 1:284903/1‑69 (MQ=255) aTGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg < 1:3289777/71‑1 (MQ=255) tGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg < 1:881300/70‑1 (MQ=255) tGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg < 1:2653277/70‑1 (MQ=255) tGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg < 1:758037/70‑1 (MQ=255) ggTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa > 1:16518/1‑70 (MQ=255) gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa < 1:725750/71‑1 (MQ=255) gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa < 1:1601256/71‑1 (MQ=255) gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCAGACATCATCATGATGTGAGCATCAGGATa < 1:2565139/71‑1 (MQ=255) caccacCAGCACCACATCTCCTTCCTGTAACAACGAAGCAGACATCATCATGATGTGAGCGTCCGGa > 1:2782745/1‑67 (MQ=255) caccacCAGCACCACATCTCCTTCCTGTAACAACGAAGCAGACATCATCATGATGTGAGCGTCCGGAt > 1:1437099/1‑68 (MQ=255) caccacCAGCACCACATCTCCTTCCTGTAACAACGAAGCAGACATCATCATGATGTGAGCGTCCGGAt > 1:1662639/1‑68 (MQ=255) cTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCatgatg < 1:589120/53‑1 (MQ=255) cTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAg < 1:752367/57‑1 (MQ=255) tACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat < 1:1160689/48‑1 (MQ=255) tACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat < 1:1415727/48‑1 (MQ=255) tACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat < 1:3028509/48‑1 (MQ=255) tACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatca > 1:2168639/1‑47 (MQ=255) aCCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGc > 1:2140592/1‑56 (MQ=255) aCCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCa > 1:173250/1‑57 (MQ=255) aCCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAg > 1:2077292/1‑66 (MQ=255) aCCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACACCATCATGATGTGAGCATCAGGATAg > 1:898095/1‑66 (MQ=255) ccAGCACCACATCTCCTTCCTGTAACAACGAAGCAGAcatc < 1:2382532/41‑1 (MQ=38) ccAGCACCACATCTCCTTCCTGTAACAACGAAGCAGAcatc < 1:2027941/41‑1 (MQ=38) ccAGCACCACATCTCCTTCCTGTAACAACGAAGCAGAcatc < 1:2687600/41‑1 (MQ=38) ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa > 1:1141139/1‑64 (MQ=255) ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTg < 1:1461501/70‑1 (MQ=255) ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGa > 1:3103676/1‑71 (MQ=255) ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGa > 1:133514/1‑71 (MQ=255) cAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCt < 1:1081154/68‑1 (MQ=255) cAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGa > 1:3791550/1‑70 (MQ=255) cAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGa > 1:2451328/1‑70 (MQ=255) gCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAg < 1:3145943/71‑1 (MQ=255) acaacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa > 1:1281639/1‑59 (MQ=255) acaacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCg < 1:954707/71‑1 (MQ=255) caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAg < 1:1867494/48‑1 (MQ=255) caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAg < 1:975321/48‑1 (MQ=255) caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa < 1:1044577/56‑1 (MQ=255) caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa < 1:678212/56‑1 (MQ=255) caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa > 1:1657331/1‑56 (MQ=255) aacaTTTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTa < 1:1156811/71‑1 (MQ=255) acaTCTCCTTCCTGTAACAACGAAGCGGACATCATCatga > 1:2557085/1‑40 (MQ=255) acaTCTCCTTCCTGTAACAACGAAGCGGACATCATCatga > 1:1037733/1‑40 (MQ=255) acaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTAc > 1:3772770/1‑71 (MQ=255) acaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTAc > 1:2217265/1‑71 (MQ=255) tccttcctGTAACAACGAAGCGGACATCATCatga > 1:2015936/1‑35 (MQ=255) tccttcctGTAACAACGAAGCGGACATCATCATGATGTGa > 1:3815293/1‑40 (MQ=255) tccttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCaa > 1:410296/1‑71 (MQ=255) tccttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCaa > 1:1943452/1‑71 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGcc < 1:2530921/67‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCa < 1:477104/68‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCa < 1:2338945/68‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt < 1:2139069/70‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt < 1:856928/70‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt < 1:1585180/70‑1 (MQ=255) cttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATg > 1:1399538/1‑71 (MQ=255) ttcctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt < 1:3348205/69‑1 (MQ=255) tcctGTAACAACGAAGCGGACATCATCATGATCTGAGCAt > 1:403059/1‑40 (MQ=255) cctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg > 1:974080/1‑42 (MQ=255) cctGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg > 1:3452319/1‑42 (MQ=255) tGTAACAACGAAGCGGACATCATCATGATGTGAGCa < 1:3625310/36‑1 (MQ=255) tGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa < 1:2242036/42‑1 (MQ=255) gTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGa < 1:1974653/41‑1 (MQ=255) aacaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGaa > 1:1201493/1‑71 (MQ=255) aacaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGaa > 1:3045571/1‑71 (MQ=255) acaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAAc > 1:1461758/1‑71 (MQ=255) acaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAAc > 1:1319633/1‑71 (MQ=255) acaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAAc > 1:292070/1‑71 (MQ=255) acaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAAc > 1:3030880/1‑71 (MQ=255) aacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACa < 1:560391/47‑1 (MQ=255) aacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACtt > 1:1868470/1‑71 (MQ=255) cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTtgt > 1:1728989/1‑71 (MQ=255) cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTtgt < 1:3623081/71‑1 (MQ=255) cGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGAAAGCGTACGCCAATGCGCAAGAACTtgt < 1:3472645/71‑1 (MQ=255) aaGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGc > 1:3414183/1‑71 (MQ=255) aGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGc > 1:1789646/1‑70 (MQ=255) aGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCt < 1:1685032/71‑1 (MQ=255) gCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGaa > 1:1509897/1‑62 (MQ=255) gCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTAGTGCTg < 1:2268925/71‑1 (MQ=255) cGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTg < 1:214239/70‑1 (MQ=255) ggACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTa < 1:816337/71‑1 (MQ=255) ggACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTa < 1:2104380/71‑1 (MQ=255) | TTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTA > W3110S.gb/4317058‑4317196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |