| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,347,163 | A→G | 10.0% | P191P (CCA→CCG) | melA → | alpha‑galactosidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,347,163 | 0 | A | G | 10.0% | 184.2 / 10.1 | 70 | P191P (CCA→CCG) | melA | alpha‑galactosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (12/51); new base G (2/5); total (14/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACC > W3110S.gb/4347095‑4347232 | cAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGc < 1:1105250/71‑1 (MQ=255)cAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGc < 1:2789693/71‑1 (MQ=255) tCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACg > 1:3857099/1‑71 (MQ=255) tCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACg > 1:1201008/1‑71 (MQ=255) tgtgCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGt > 1:1475682/1‑71 (MQ=255) ccATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGc < 1:464728/57‑1 (MQ=255) aTTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGt > 1:1845971/1‑71 (MQ=255) tGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCa < 1:1104536/71‑1 (MQ=255) gggAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTa < 1:2012970/71‑1 (MQ=255) ggAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTgcg > 1:2598449/1‑64 (MQ=255) gAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATc < 1:2664296/71‑1 (MQ=255) gAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATc < 1:1031829/71‑1 (MQ=255) aaCGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTa > 1:868738/1‑44 (MQ=255) aaCGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTAcc < 1:1918294/56‑1 (MQ=255) aaCGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTAcc < 1:3857608/56‑1 (MQ=255) aaCGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTAcc < 1:3853438/56‑1 (MQ=255) ggcggAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTAcc < 1:3726180/53‑1 (MQ=255) ggcggAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAAcc < 1:589114/71‑1 (MQ=255) ggcggAAGAGCTGGCGCGTGACCTCAATATCGACCCGGCTACGCTGc < 1:631911/47‑1 (MQ=38) ggcggAAGAGCTGGCGCGTGACCTCAATATCGACCCGGCTACGCTGc < 1:692979/47‑1 (MQ=38) ggcggAAGAGCTGGCGCGTGACCTCAATATCGACCCGGCTACGCTGc < 1:2016712/47‑1 (MQ=38) ggcggAAGAGCTGGCGCGTGACCTCAATATCGACCCGGCTACGCTGc < 1:2435843/47‑1 (MQ=38) ggcggAAGAGCTGGCGCCTGACCTCAATATCGACCCGGCTACGCTGc < 1:1171026/47‑1 (MQ=38) gcggAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:574258/71‑1 (MQ=255) gcggAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:934856/71‑1 (MQ=255) cggAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCat < 1:585930/71‑1 (MQ=255) gAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCg < 1:1571980/50‑1 (MQ=255) aaGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATg < 1:2438611/71‑1 (MQ=255) aaGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATg < 1:1699292/71‑1 (MQ=255) agTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCg < 1:1942630/71‑1 (MQ=255) agTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCg < 1:1128791/71‑1 (MQ=255) agTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCg < 1:3339676/71‑1 (MQ=255) gTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCg < 1:699610/70‑1 (MQ=255) ttGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAAcaa < 1:199360/62‑3 (MQ=255) ttGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:3637504/62‑1 (MQ=255) ttGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:3633071/62‑1 (MQ=255) ttGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTAACGTTGCGCAGGTATCAACCa < 1:3852198/62‑1 (MQ=255) tGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCat < 1:2648517/62‑1 (MQ=255) ggCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGttt < 1:937651/70‑1 (MQ=255) ggCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGttt < 1:870079/70‑1 (MQ=255) ggCGCGTGACATCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGttt < 1:3061859/70‑1 (MQ=255) gcgcGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGtttt < 1:56220/70‑1 (MQ=255) cgcgTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTAc < 1:2050226/71‑1 (MQ=255) cgcgTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTAc < 1:580934/71‑1 (MQ=255) gcgTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTAcc < 1:3503731/71‑1 (MQ=255) gcgTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTAc < 1:3296822/70‑1 (MQ=255) gTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCata > 1:2743535/1‑57 (MQ=255) gTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCata > 1:1296350/1‑57 (MQ=255) gTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGAAGGTATCAACCata > 1:1971731/1‑57 (MQ=255) gTGACCTCAATATCGACACAGCTACGCTGCGTTACCGTTGCGCAg > 1:181450/1‑45 (MQ=255) cTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:316205/50‑1 (MQ=255) cTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGtttt < 1:232645/61‑1 (MQ=255) cTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACctgg < 1:327283/67‑1 (MQ=255) cTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACctgg < 1:1750778/67‑1 (MQ=255) cTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACctgg < 1:1533806/67‑1 (MQ=255) cAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGtttt < 1:1134357/59‑1 (MQ=255) atatCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACctgg < 1:651952/63‑1 (MQ=255) atCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAgcg > 1:2439156/1‑71 (MQ=255) tCGACCCGGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAgcg > 1:3099301/1‑70 (MQ=255) tCGACCCGGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAgcg > 1:455572/1‑70 (MQ=255) gACCCAGCTACGCTGCGTTACCGTTGCTCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCa > 1:3770825/1‑70 (MQ=255) gACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCaa < 1:583122/71‑1 (MQ=255) gACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCaa < 1:2851244/71‑1 (MQ=255) gACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCaa < 1:2611066/71‑1 (MQ=255) gACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCaa < 1:1097378/71‑1 (MQ=255) cAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:540272/37‑1 (MQ=255) cAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:516196/37‑1 (MQ=255) cAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCa < 1:3858588/37‑1 (MQ=255) cAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAAcc < 1:815255/71‑1 (MQ=255) cAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAAcc < 1:3232344/71‑1 (MQ=255) | CAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGTTGGCGCGTGACCTCAATATCGACCCAGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACC > W3110S.gb/4347095‑4347232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |