| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,375,910 | T→C | 8.2% | N68N (AAT→AAC) | groL → | Cpn60 chaperonin GroEL, large subunit of GroESL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,375,910 | 0 | T | C | 8.2% | 163.6 / 5.0 | 61 | N68N (AAT→AAC) | groL | Cpn60 chaperonin GroEL, large subunit of GroESL |
| Reads supporting (aligned to +/- strand): ref base T (43/13); new base C (2/3); total (45/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
GTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCAC > W3110S.gb/4375840‑4375978 | gTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAat < 1:2829045/71‑1 (MQ=255)gTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAat < 1:1392036/71‑1 (MQ=255)gTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAat < 1:3268812/71‑1 (MQ=255)gTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAat < 1:1758619/71‑1 (MQ=255) aCCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTgc > 1:117504/1‑71 (MQ=255) tCACCAAAGATGGTGTTTCCGTTTCTCGTGAAATCGAACTTGAAGACAAGTTAGAAAatat < 1:3720998/61‑1 (MQ=255) tCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAatat < 1:671510/61‑1 (MQ=255) aCCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGa < 1:142581/70‑1 (MQ=255) aCCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGAt < 1:84140/71‑1 (MQ=255) cAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAata > 1:434547/1‑56 (MQ=255) cAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAata > 1:1437428/1‑56 (MQ=255) aaGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAata > 1:3459367/1‑54 (MQ=255) aaGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAata > 1:2967734/1‑54 (MQ=255) aaGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGTGCGCAg > 1:834698/1‑65 (MQ=255) aaGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGTGCGCAg > 1:2231987/1‑65 (MQ=255) aTGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGa > 1:2740555/1‑69 (MQ=255) tGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaag < 1:3009832/71‑1 (MQ=255) tGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaag < 1:1427927/71‑1 (MQ=255) gtgtTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGa > 1:423832/1‑60 (MQ=255) gtgtTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaaga < 1:2375206/70‑1 (MQ=255) ttCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTg > 1:3272124/1‑71 (MQ=255) tCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaa < 1:137516/66‑1 (MQ=255) tCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaa < 1:535827/66‑1 (MQ=255) ttGCTCTTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaaga < 1:2807944/61‑1 (MQ=255) ttGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCctc > 1:3079814/1‑70 (MQ=255) ttGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCctc > 1:151070/1‑70 (MQ=255) tGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaag > 1:2321706/1‑62 (MQ=255) tGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaag > 1:3409206/1‑62 (MQ=255) tGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaag > 1:1805706/1‑62 (MQ=255) tGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaagaag > 1:889901/1‑62 (MQ=255) cGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAg > 1:1828786/1‑70 (MQ=255) cGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAg > 1:3793502/1‑70 (MQ=255) gTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGc > 1:1784192/1‑70 (MQ=255) tGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCa > 1:315109/1‑70 (MQ=255) tGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCa > 1:3329936/1‑70 (MQ=255) tGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCa > 1:428645/1‑70 (MQ=255) gAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCaaa > 1:738253/1‑71 (MQ=255) aaaTCGAACTGGAGGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAac > 1:2723486/1‑71 (MQ=255) aaaTCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAac > 1:2805474/1‑71 (MQ=255) aaaTCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAac > 1:3300775/1‑71 (MQ=255) aaaTCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAac > 1:3350954/1‑71 (MQ=255) tCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTCCCTCTAAAGCAAacgac > 1:3582531/1‑71 (MQ=255) cGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAacgacg > 1:1773366/1‑71 (MQ=255) cGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAacgacg > 1:1098934/1‑71 (MQ=255) aCTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTg > 1:1745502/1‑71 (MQ=255) tGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGtt < 1:3056217/47‑1 (MQ=255) tGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGc < 1:2197761/49‑1 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCa > 1:77550/1‑69 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAgg > 1:746888/1‑71 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAgg > 1:1656899/1‑71 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAgg > 1:1523315/1‑71 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAg > 1:2772585/1‑70 (MQ=255) gAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCT‑‑AGCAAACGACGCTGc > 1:902457/1‑66 (MQ=255) aGACAAGTTCGAAAACATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCg < 1:3622824/71‑1 (MQ=255) aGACAAGTTCGAAAACATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCg < 1:1706684/71‑1 (MQ=255) aGACAAGTTCGAAAACATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCg < 1:643757/71‑1 (MQ=255) gACAAGTTCGAAAATATGGGTGCGCAGATGGTGAaaga < 1:3206116/38‑1 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:3712648/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:1348253/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:157773/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:2289072/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:3003225/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGt > 1:3005558/1‑39 (MQ=255) cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGAc < 1:219977/70‑1 (MQ=255) aGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGt < 1:1892772/71‑1 (MQ=255) ttCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTac > 1:1120003/1‑71 (MQ=255) cGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAg > 1:2756680/1‑44 (MQ=255) aaTATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGt > 1:2551543/1‑63 (MQ=255) aaTATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACcaccac > 1:2397479/1‑71 (MQ=255) aaTATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACcaccac > 1:1464848/1‑71 (MQ=255) aaTATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACcaccac > 1:895741/1‑71 (MQ=255) | GTGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCAC > W3110S.gb/4375840‑4375978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |