| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,379,696 | C→T | 11.7% | R215H (CGT→CAT) | yjeK ← | predicted lysine aminomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,379,696 | 0 | C | T | 11.7% | 136.3 / 7.6 | 51 | R215H (CGT→CAT) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base C (20/25); new base T (3/3); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCG > W3110S.gb/4379631‑4379766 | ggAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGc > 1:1097584/1‑70 (MQ=255)ggAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCa < 1:7039/71‑1 (MQ=255) gAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCaa < 1:3168177/71‑1 (MQ=255) gAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCaa < 1:1556444/71‑1 (MQ=255) aTGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAg < 1:128711/71‑1 (MQ=255) tGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAg < 1:78921/70‑1 (MQ=255) tttCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCAt > 1:826537/1‑71 (MQ=255) tCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCa > 1:2666151/1‑68 (MQ=255) tCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCAtt < 1:3516906/70‑1 (MQ=255) tCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCAtt > 1:1869800/1‑70 (MQ=255) aTCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCa < 1:3783529/70‑1 (MQ=255) cATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:3742029/1‑63 (MQ=255) cATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:1792678/1‑63 (MQ=255) cATTGGCATGGTTCATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGcc > 1:2295229/1‑71 (MQ=255) aTTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAgcgc < 1:1091189/69‑1 (MQ=255) aTTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCt > 1:1820663/1‑71 (MQ=255) aTTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTACAACGGGCAAAGCATTCAACCAGCGCCt > 1:984123/1‑71 (MQ=255) tGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTc < 1:490655/70‑1 (MQ=255) ggCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCgg > 1:2469887/1‑71 (MQ=255) tGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGc < 1:3716945/49‑1 (MQ=255) tGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGc < 1:820528/49‑1 (MQ=255) tGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGa < 1:258403/70‑1 (MQ=255) tGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGAt < 1:1847877/71‑1 (MQ=255) gTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATAc > 1:1658579/1‑71 (MQ=255) gATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAg > 1:2096920/1‑43 (MQ=255) gATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAg > 1:942005/1‑43 (MQ=255) gATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGt < 1:3724182/70‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:1095925/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:512688/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:2075053/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGcc < 1:3761002/71‑1 (MQ=255) gtgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCgg > 1:2895965/1‑59 (MQ=255) tgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:106233/1‑46 (MQ=255) tgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:742115/1‑46 (MQ=255) tgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:3300648/1‑46 (MQ=255) tgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCaa > 1:2932093/1‑46 (MQ=255) aTTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCggg < 1:3584484/70‑1 (MQ=255) ttCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGGATTCAACCAGCGCCTCGGTGATACGTGCCGGGAt < 1:2258834/71‑1 (MQ=255) aCCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATca < 1:3578353/70‑1 (MQ=255) ccAGCAAGATTTGCAGTGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATcaca < 1:517779/71‑1 (MQ=255) ccAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATcaca < 1:761821/71‑1 (MQ=255) cAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACaa < 1:1358240/71‑1 (MQ=255) aaGATTTGCAGCGTAGAATGTGAAAAGCGTTCAAc > 1:992329/1‑35 (MQ=19) aaGATTTGCAGCGTAGAATGTGAAAAGCGTTCAAc > 1:583809/1‑35 (MQ=19) aaGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCgg < 1:3726326/71‑1 (MQ=255) aGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCgg > 1:3109006/1‑70 (MQ=255) tttGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATa < 1:1131009/47‑1 (MQ=255) tGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGCCGGGAt > 1:1430725/1‑56 (MQ=255) tAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGa < 1:3240926/71‑1 (MQ=255) tAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGa < 1:1401494/71‑1 (MQ=255) aGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGaa > 1:200072/1‑71 (MQ=255) cGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCg < 1:3059175/71‑1 (MQ=255) | GGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCG > W3110S.gb/4379631‑4379766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |