| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,379,728 | A→G | 13.6% | R204R (CGT→CGC) | yjeK ← | predicted lysine aminomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,379,728 | 0 | A | G | 13.6% | 105.4 / 8.9 | 44 | R204R (CGT→CGC) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base A (15/23); new base G (3/3); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
GTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGCTTCCAGT > W3110S.gb/4379659‑4379797 | gTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATAc > 1:1658579/1‑71 (MQ=255) gATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGt < 1:3724182/70‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:1095925/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:512688/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGcc < 1:2075053/71‑1 (MQ=255) tgtgATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGcc < 1:3761002/71‑1 (MQ=255) aTTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCggg < 1:3584484/70‑1 (MQ=255) ttCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGGATTCAACCAGCGCCTCGGTGATACGTGCCGGGAt < 1:2258834/71‑1 (MQ=255) aCCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATca < 1:3578353/70‑1 (MQ=255) ccAGCAAGATTTGCAGTGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATcaca < 1:517779/71‑1 (MQ=255) ccAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATcaca < 1:761821/71‑1 (MQ=255) cAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACaa < 1:1358240/71‑1 (MQ=255) aaGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCgg < 1:3726326/71‑1 (MQ=255) aGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCgg > 1:3109006/1‑70 (MQ=255) tttGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATa < 1:1131009/47‑1 (MQ=255) tGCAGCGTAGAATGTGAAAAGCGTTCAACCAGCGCATCTGTGATGCGTGCCGGGAt > 1:1430725/1‑56 (MQ=255) tAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGa < 1:1401494/71‑1 (MQ=255) tAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGa < 1:3240926/71‑1 (MQ=255) aGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGaa > 1:200072/1‑71 (MQ=255) cGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCg < 1:3059175/71‑1 (MQ=255) gggCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATcaca < 1:3766599/47‑1 (MQ=255) gggCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCg > 1:208526/1‑70 (MQ=255) gggCAAAGCATTCAACCAGCGACTCGGTGATACGTGCCGGGATcaca < 1:419957/47‑1 (MQ=255) aaGCATTCAACGAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACg > 1:2517910/1‑71 (MQ=255) aaGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACg > 1:1381942/1‑71 (MQ=255) aGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGCATCCGCAGACGt > 1:3734849/1‑71 (MQ=255) cATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGttt < 1:1991477/71‑1 (MQ=255) ttCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAg < 1:147809/63‑1 (MQ=255) ttCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGtttt < 1:1075261/70‑1 (MQ=255) cAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtata > 1:936445/1‑71 (MQ=255) cAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtata > 1:1006390/1‑71 (MQ=255) cAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtata > 1:3251905/1‑71 (MQ=255) cAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtat < 1:2172407/70‑1 (MQ=255) cAACCAGCGACTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtata > 1:1250179/1‑71 (MQ=255) aaCCAGCGCATCTGTGATGCGTGCCGGGATCACAATCGGCAGACGACTGTGAATCCGCAGACGTTTGatat > 1:258550/1‑71 (MQ=255) aaCCAGCGCATCTGTGATGCGTGCCGGGATCACAATCGGCAGACCACTGTGAATCCGCAGACGTTTgata > 1:1375135/1‑70 (MQ=255) aGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCgg < 1:1858938/71‑1 (MQ=255) tCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTta < 1:3287292/58‑1 (MQ=255) tCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGAGGGATGGc > 1:3847107/1‑71 (MQ=255) tCGGTGATACGTGCCGGGATCACAATCGCCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGc > 1:3803902/1‑71 (MQ=255) ggTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGaa > 1:3651165/1‑41 (MQ=255) ggTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGCtt > 1:1457185/1‑71 (MQ=255) gATACTTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTtat < 1:392825/54‑1 (MQ=255) aTACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGCTTCCa < 1:2621224/70‑1 (MQ=255) tACGTGCCGTGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGCTTCCAg < 1:3512363/70‑1 (MQ=255) aCGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGATTTATATGCGGGATGGCTTCCAGt > 1:3562050/1‑70 (MQ=255) | GTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGATCACAATCGGCAGACGGCTGTGAATCCGCAGACGTTTTATATGCGGGATGGCTTCCAGT > W3110S.gb/4379659‑4379797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |