Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,436,156 A→G 6.6% I170I (ATT→ATC ytfE ← predicted regulator of cell morphogenesis and cell wall metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,436,1560AG 6.6% 162.1 / 2.6 60I170I (ATT→ATCytfEpredicted regulator of cell morphogenesis and cell wall metabolism
Reads supporting (aligned to +/- strand):  ref base A (19/37);  new base G (2/2);  total (21/39)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGG  >  W3110S.gb/4436088‑4436225
                                                                    |                                                                     
ccGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAAt                                                                     <  1:718038/71‑1 (MQ=255)
 cGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATc                                                                    <  1:92025/71‑1 (MQ=255)
 cGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATc                                                                    <  1:857256/71‑1 (MQ=255)
 cGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATc                                                                    <  1:3167175/71‑1 (MQ=255)
   ttATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAAt                                                                     <  1:1157344/68‑1 (MQ=255)
     ataCATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACtt                                                                >  1:3686890/1‑71 (MQ=255)
      taCATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTc                                                               <  1:212814/71‑1 (MQ=255)
          tCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTc                                                               <  1:2716318/67‑1 (MQ=255)
          tCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTc                                                               <  1:1692345/67‑1 (MQ=255)
          tCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCca                                                             <  1:2625934/69‑1 (MQ=255)
           cGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCAc                                                                  >  1:608079/1‑63 (MQ=255)
           cGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCcag                                                            >  1:3065958/1‑69 (MQ=255)
            gCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCcagcag                                                         <  1:1033874/71‑1 (MQ=255)
             cTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGt                                                        <  1:3171746/71‑1 (MQ=255)
                  cAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGcc                                                   <  1:1115924/71‑1 (MQ=255)
                  cAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGcc                                                   <  1:1764765/71‑1 (MQ=255)
                    ggtggtGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGccc                                                  <  1:1693935/70‑1 (MQ=255)
                        gtgCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCtt                                              <  1:1327572/70‑1 (MQ=255)
                            aGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCg                                         <  1:2748545/71‑1 (MQ=255)
                              gCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCtt                                              <  1:2632345/64‑1 (MQ=255)
                               cTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGc                                      <  1:715653/71‑1 (MQ=255)
                               cTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGc                                      >  1:2641624/1‑71 (MQ=255)
                               cTTCTGGCGGCGGTGTGACGTTATTGGTGGTATGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGc                                      >  1:185822/1‑71 (MQ=255)
                                ttCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCa                                                                   >  1:797329/1‑41 (MQ=255)
                                ttCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCcagc                                                           <  1:2108502/49‑1 (MQ=255)
                                  cTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTc                                    <  1:2241512/70‑1 (MQ=255)
                                      cggcggTGTGACGTTATTGGTGGTGTGTTTGATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCttt                               >  1:1306271/1‑71 (MQ=255)
                                      cggcggTGTGACGTTATTGGTGGTGTGTTTGATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCttt                               >  1:2886604/1‑71 (MQ=255)
                                       ggcggTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTc                              <  1:1485141/71‑1 (MQ=255)
                                       ggcggTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTc                              <  1:2183963/71‑1 (MQ=255)
                                        gcggTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTc                              >  1:517206/1‑70 (MQ=255)
                                           gtgtGACGTTATTGGTGGTGTGTTTAATCACTTCcagcag                                                         <  1:1023846/40‑1 (MQ=255)
                                           gtgtGACGTTATTGGTGGTGTGTTTAATCACTTCcagcag                                                         <  1:1465572/40‑1 (MQ=255)
                                              tgACGTTATTGGTGGTGTGTTTAATCACTTCcagcag                                                         <  1:738324/37‑1 (MQ=255)
                                              tgACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCttt                               <  1:2719324/63‑1 (MQ=255)
                                                aCGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTc                                             >  1:1854352/1‑47 (MQ=255)
                                                 cGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTg                    <  1:1618363/71‑1 (MQ=255)
                                                  gTTATTGGTGGTGTGTTTGATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                   <  1:3694620/71‑1 (MQ=255)
                                                  gTTATTGGTGGTGTGTTTGATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                   <  1:1890576/71‑1 (MQ=255)
                                                  gTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                   <  1:792454/71‑1 (MQ=255)
                                                  gTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                   <  1:195364/71‑1 (MQ=255)
                                                  gTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGa                   <  1:87242/71‑1 (MQ=255)
                                                  gTTATTGGGGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGGtt                                              <  1:1429336/44‑1 (MQ=38)
                                                   ttATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCtt                                              <  1:2143599/43‑1 (MQ=255)
                                                    tATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCtt                                              <  1:3327647/42‑1 (MQ=255)
                                                     aTTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGc                                                    >  1:819841/1‑35 (MQ=255)
                                                     aTTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTg                >  1:2885626/1‑71 (MQ=255)
                                                     aTTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTg                >  1:1711045/1‑71 (MQ=255)
                                                     aTTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTg                >  1:1244139/1‑71 (MQ=255)
                                                     aTTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTg                >  1:1727006/1‑71 (MQ=255)
                                                        ggtggtGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCTCTTTCCATTACGCTGATTGGcc             >  1:911747/1‑71 (MQ=255)
                                                        ggtggtGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGcc             >  1:3398369/1‑71 (MQ=255)
                                                              gtgtTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCtt                                <  1:221362/46‑1 (MQ=255)
                                                              gtgtTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCtt                                <  1:3811037/46‑1 (MQ=255)
                                                               tgtTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTcc                             >  1:1758480/1‑48 (MQ=255)
                                                               tgtTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTcc                             >  1:1621869/1‑48 (MQ=255)
                                                               tgtTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGc      <  1:1380702/71‑1 (MQ=255)
                                                               tgtTTAATCACTTCCAGCAGTTCGCCCCTTCATCGTGCTCGCTTTcc                             >  1:2149193/1‑47 (MQ=38)
                                                                 tttAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGcc     <  1:397945/70‑1 (MQ=255)
                                                                   tAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTgg  >  1:1219411/1‑71 (MQ=255)
                                                                    |                                                                     
CCGTTATACATCGCTTTCCAGGTGGTGCAGGCTTCTGGCGGCGGTGTGACGTTATTGGTGGTGTGTTTAATCACTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGG  >  W3110S.gb/4436088‑4436225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: