| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,437,511 | G→A | 7.8% | L79L (CTC→CTT) | ytfF ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,437,511 | 0 | G | A | 7.8% | 188.5 / 4.4 | 64 | L79L (CTC→CTT) | ytfF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (27/32); new base A (2/3); total (29/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
CCGATAATCATCGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACG > W3110S.gb/4437443‑4437580 | ccGATAATCATCGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAg > 1:1046024/1‑71 (MQ=255) gATAATCATCGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGt > 1:880129/1‑71 (MQ=255) aaTCATCGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGc > 1:1710666/1‑71 (MQ=255) catcGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCca > 1:3833947/1‑71 (MQ=255) atcGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcat > 1:2170662/1‑71 (MQ=255) atcGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcat > 1:166268/1‑71 (MQ=255) atcGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcat > 1:1606715/1‑71 (MQ=255) atcGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCca > 1:3221721/1‑70 (MQ=255) atcGTGGAAACAGGAGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcat > 1:2913676/1‑71 (MQ=255) gTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:1530110/70‑1 (MQ=255) gTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:703577/70‑1 (MQ=255) gTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:1525697/70‑1 (MQ=255) gTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca > 1:1344339/1‑70 (MQ=255) aaCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:1549480/65‑1 (MQ=255) aaCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:2198211/65‑1 (MQ=255) aaCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:1321632/65‑1 (MQ=255) aaCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCcatca < 1:1088543/65‑1 (MQ=255) aCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:1514531/1‑66 (MQ=255) aCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:772516/1‑66 (MQ=255) cAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAg > 1:189061/1‑70 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCAt > 1:2334791/1‑70 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:3839367/71‑1 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:1838151/71‑1 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:777800/71‑1 (MQ=255) cgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc > 1:279377/1‑70 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGt < 1:830044/71‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGt < 1:512999/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGa < 1:3060139/36‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:615972/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:1666738/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:3762403/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:817051/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:2077232/71‑1 (MQ=255) tGAATGGCACTTGCAAGTCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGc > 1:2498504/1‑69 (MQ=255) tGAATGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGc > 1:759624/1‑69 (MQ=255) tGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc > 1:3495774/1‑69 (MQ=255) gAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:3842781/1‑47 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:999264/71‑1 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:3078672/71‑1 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:2542064/71‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:2626160/70‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc > 1:1302912/1‑70 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:3742073/70‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:45011/70‑1 (MQ=255) cAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGcc < 1:1377099/71‑1 (MQ=255) cAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGcc < 1:3749591/71‑1 (MQ=255) aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa < 1:3258513/71‑1 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt > 1:137144/1‑70 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg < 1:1996665/71‑1 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg > 1:2202339/1‑71 (MQ=255) aGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg < 1:2506414/70‑1 (MQ=255) aaGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg < 1:2130992/71‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:1283567/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:2155478/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:3861104/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAc < 1:373621/52‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAc < 1:1922906/52‑1 (MQ=255) aGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg > 1:3163839/1‑64 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:2354769/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:1698869/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:3616567/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgcagacgca > 1:2570562/1‑71 (MQ=255) tGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacg > 1:1853272/1‑71 (MQ=255) tGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacg > 1:3600983/1‑71 (MQ=255) | CCGATAATCATCGTGGAAACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACG > W3110S.gb/4437443‑4437580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |