| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 427,941 | T→C | 11.6% | T357T (ACT→ACC) | secD → | SecYEG protein translocase auxillary subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 427,941 | 0 | T | C | 11.6% | 216.9 / 17.2 | 86 | T357T (ACT→ACC) | secD | SecYEG protein translocase auxillary subunit |
| Reads supporting (aligned to +/- strand): ref base T (39/37); new base C (5/5); total (44/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCA > W3110S.gb/427876‑428010 | tACAACCAGCCGCAGGTTAACATCTCGCTCGATAGCGCGGGTGGTAACATCATGTCTAACTTCACCAAGGa < 1:22868/71‑1 (MQ=255)tACAACCAGCCGCAGGTTAACATCTCGCTCGATAGCGCGGGTGGTAACATCATGTCTAACTTCACCAAGGa < 1:1969223/71‑1 (MQ=255)tACAACCAGCCGCAGGTTAACATCTCGCTCGATAGCGCGGGTGGTAACATCATGTCTAACTTCACCAAGGa < 1:1274351/71‑1 (MQ=255)tACAACCAGCCGCAGGTTAACATCTCGCTCGATAGCGCGGGTGGTAACATCATGTCTAACTTCACCAAGGa < 1:903108/71‑1 (MQ=255)tACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAgg > 1:3716776/1‑70 (MQ=255) caaccaacCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGac > 1:109330/1‑70 (MQ=255) caaccaacCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGac < 1:1619645/70‑1 (MQ=255) accaaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGacaa > 1:1749439/1‑70 (MQ=255) aaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACAt > 1:876990/1‑70 (MQ=255) aaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACAt > 1:2763725/1‑70 (MQ=255) aaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATc > 1:405578/1‑71 (MQ=255) aaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATc < 1:751270/71‑1 (MQ=255) aaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATc < 1:307671/71‑1 (MQ=255) cAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTaa > 1:1765957/1‑56 (MQ=255) cAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCa > 1:2610595/1‑70 (MQ=255) gTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTa > 1:1707752/1‑52 (MQ=255) gTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc < 1:1081301/71‑1 (MQ=255) gTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc < 1:566724/71‑1 (MQ=255) gTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc < 1:3444172/71‑1 (MQ=255) ttAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc > 1:3179847/1‑70 (MQ=255) tAACATCTCGCTCGATAGCGCTTGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCg > 1:3700472/1‑70 (MQ=255) tAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGa > 1:2422771/1‑71 (MQ=255) aaCATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTa < 1:1658315/49‑1 (MQ=255) aaCATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGAt > 1:1775835/1‑71 (MQ=255) cATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATgg > 1:503675/1‑71 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCaa < 1:162846/62‑1 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc < 1:2713999/65‑1 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGa < 1:2797750/67‑1 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGa < 1:3856153/67‑1 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATTGc < 1:1414001/71‑1 (MQ=255) aTCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGc < 1:3573439/71‑1 (MQ=255) tctcGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCa < 1:768632/60‑1 (MQ=255) tcgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAAc > 1:603189/1‑71 (MQ=255) gctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCa < 1:3583466/67‑1 (MQ=255) gctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCa < 1:2281790/67‑1 (MQ=255) tcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGac < 1:1001550/44‑1 (MQ=255) tcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGac < 1:2281809/44‑1 (MQ=255) tcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGac < 1:2559475/44‑1 (MQ=255) tAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGacaaca > 1:234826/1‑44 (MQ=255) tAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtg < 1:3035357/71‑1 (MQ=255) aGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgt > 1:1844324/1‑71 (MQ=255) gcgcTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAAcc > 1:3425870/1‑52 (MQ=255) gtggtAACATCATGTCTAACTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTa > 1:2556669/1‑70 (MQ=255) ggtAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACa > 1:3110449/1‑70 (MQ=255) gtAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACaaa < 1:1805252/71‑1 (MQ=255) gtAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACaaa < 1:1823909/71‑1 (MQ=255) tAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGa < 1:2179082/44‑1 (MQ=255) tAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAg < 1:3128021/71‑1 (MQ=255) aaCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCaa > 1:935256/1‑49 (MQ=255) aaCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCaa > 1:1196425/1‑49 (MQ=255) aaCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtg > 1:3364491/1‑58 (MQ=255) aaCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtg > 1:3843115/1‑58 (MQ=255) aaCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGa > 1:2571893/1‑71 (MQ=255) aCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt > 1:3056387/1‑55 (MQ=255) aCATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt > 1:3666219/1‑55 (MQ=255) tcatGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg < 1:1769076/56‑1 (MQ=255) tcatGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg < 1:1927360/56‑1 (MQ=255) tcatGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg < 1:1421878/56‑1 (MQ=255) tcatGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg < 1:2579287/56‑1 (MQ=255) atGTCTAACTTCACTAAGGACAACATCGGCAAACCGATgg > 1:1374879/1‑40 (MQ=255) atGTCTAACTTCACTAAGGACAACATCGGCAAACCGATg > 1:1401563/1‑39 (MQ=255) atGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt > 1:3770642/1‑50 (MQ=255) atGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt > 1:925265/1‑50 (MQ=255) tGTCTAACTTCACTAAGGACAACATCGGCAAACCGa > 1:3812028/1‑36 (MQ=255) tGTCTAACTTCACTAAGGACAACATCGGCAAACCGa > 1:3078780/1‑36 (MQ=255) tGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGt < 1:723080/71‑1 (MQ=255) tGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGt < 1:2873348/71‑1 (MQ=255) gTCTAACTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTACAAAGAc < 1:2765883/64‑1 (MQ=255) taacttaactAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTaag > 1:1511823/1‑70 (MQ=255) tAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt < 1:3154222/45‑1 (MQ=255) tAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt < 1:2939873/45‑1 (MQ=255) tAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGtt < 1:2885395/45‑1 (MQ=255) tAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTtgtg > 1:2011155/1‑49 (MQ=255) tAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTaag > 1:1181367/1‑70 (MQ=255) aaCTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTACAAAGACAGCGGTaaga > 1:2567949/1‑70 (MQ=255) aaCTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTACAAAGACAGCGGTaaga > 1:2453267/1‑70 (MQ=255) aaCTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTACAAAGACAGCGGTaaga > 1:1563661/1‑70 (MQ=255) aaCTTCACCAAGGACAACATCGGCAAACCGATGGCAACTCTGTTTGTGGAGTACAAAGACAGCGGTaag > 1:2709605/1‑69 (MQ=255) aCTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTa > 1:2528803/1‑52 (MQ=255) aCTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTa > 1:1056706/1‑52 (MQ=255) aCTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACa > 1:3383671/1‑54 (MQ=255) aCTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACa > 1:794609/1‑54 (MQ=255) aCTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACa > 1:1503514/1‑54 (MQ=255) aCTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGc < 1:932340/71‑1 (MQ=255) cTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATg < 1:2926413/69‑1 (MQ=255) cTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCa < 1:748544/71‑1 (MQ=255) cTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCa < 1:1699013/71‑1 (MQ=255) | TACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCA > W3110S.gb/427876‑428010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |