| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,437,526 | A→G | 7.6% | T74T (ACT→ACC) | ytfF ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,437,526 | 0 | A | G | 7.6% | 175.6 / 4.9 | 66 | T74T (ACT→ACC) | ytfF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (27/34); new base G (2/3); total (29/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
ACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGC > W3110S.gb/4437461‑4437594 | aCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:772516/1‑66 (MQ=255)aCAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:1514531/1‑66 (MQ=255) cAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAg > 1:189061/1‑70 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCAt > 1:2334791/1‑70 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:777800/71‑1 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:1838151/71‑1 (MQ=255) gcgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:3839367/71‑1 (MQ=255) cgcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc > 1:279377/1‑70 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGt < 1:830044/71‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGt < 1:512999/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:1666738/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:3762403/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:615972/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:817051/71‑1 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:2077232/71‑1 (MQ=255) tGAATGGCACTTGCAAGTCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGc > 1:2498504/1‑69 (MQ=255) tGAATGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGc > 1:759624/1‑69 (MQ=255) tGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc > 1:3495774/1‑69 (MQ=255) gAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATa > 1:3842781/1‑47 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:3078672/71‑1 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:2542064/71‑1 (MQ=255) tGGCACTTGCAAGGCAGAAGTAATAGATAAGGTTGCCCATCATGGTGAGCATCAAGGCGGTAAGCCAGTcg < 1:999264/71‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:3742073/70‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc > 1:1302912/1‑70 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:2626160/70‑1 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:45011/70‑1 (MQ=255) cAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGcc < 1:3749591/71‑1 (MQ=255) cAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGcc < 1:1377099/71‑1 (MQ=255) aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa < 1:3258513/71‑1 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt > 1:137144/1‑70 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg > 1:2202339/1‑71 (MQ=255) cAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg < 1:1996665/71‑1 (MQ=255) aGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTg < 1:2506414/70‑1 (MQ=255) aaGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg < 1:2130992/71‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:2155478/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:3861104/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:1283567/49‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAc < 1:1922906/52‑1 (MQ=255) tAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAc < 1:373621/52‑1 (MQ=255) aGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg > 1:3163839/1‑64 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:1698869/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:2354769/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcgc > 1:3616567/1‑52 (MQ=255) gATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgcagacgca > 1:2570562/1‑71 (MQ=255) tGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacg > 1:1853272/1‑71 (MQ=255) tGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacg > 1:3600983/1‑71 (MQ=255) aGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGa < 1:2923452/45‑1 (MQ=255) ggTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc > 1:3346677/1‑53 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAc < 1:1698433/44‑1 (MQ=255) tGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTcc > 1:1012880/1‑70 (MQ=255) gCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgac > 1:1624650/1‑53 (MQ=255) gCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgac > 1:3004263/1‑53 (MQ=255) gCCCATCATAGTGAGCATCAAGGCGGTAAGCAAGTCGCGACGCGCCAACTgac > 1:1886980/1‑53 (MQ=255) cccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgcagacg < 1:2491155/59‑1 (MQ=255) atcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:292757/52‑1 (MQ=255) atcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc > 1:1141704/1‑51 (MQ=255) atcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc > 1:1966571/1‑51 (MQ=255) tcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgcag < 1:1814970/52‑1 (MQ=255) tcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgctcgc < 1:533736/60‑1 (MQ=255) tcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacgc < 1:3175043/60‑1 (MQ=255) tcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGacgcacgc < 1:9312/60‑1 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcg > 1:3213933/1‑35 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGAcg > 1:3713299/1‑35 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc > 1:2480740/1‑42 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgcagacgca > 1:3562397/1‑55 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagccag > 1:2899660/1‑69 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:3195315/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc < 1:1773835/71‑1 (MQ=255) tAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:1142475/48‑1 (MQ=255) | ACAGGCGCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGC > W3110S.gb/4437461‑4437594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |