| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,597,394 | C→T | 9.0% | R352R (CGC→CGT) | tsr → | methyl‑accepting chemotaxis protein I, serine sensor receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,597,394 | 0 | C | T | 9.0% | 155.7 / 4.0 | 55 | R352R (CGC→CGT) | tsr | methyl‑accepting chemotaxis protein I, serine sensor receptor |
| Reads supporting (aligned to +/- strand): ref base C (29/21); new base T (2/3); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCGTTAAGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTCCAGACCA > W3110S.gb/4597324‑4597464 | tGGCGTTAAGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATgcgc > 1:3105448/1‑71 (MQ=255)tGGCGTTAAGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATgcgc > 1:1710268/1‑71 (MQ=255) gCGTTAAGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGa < 1:2946532/71‑1 (MQ=255) aaGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATAtct > 1:733763/1‑71 (MQ=255) aaGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATAtct > 1:3706043/1‑71 (MQ=255) cTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTccac > 1:620620/1‑70 (MQ=255) cTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGt > 1:1378973/1‑71 (MQ=255) aaaCGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCg > 1:446876/1‑71 (MQ=255) ggCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGat < 1:3201397/52‑1 (MQ=255) ggCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGat < 1:2618154/52‑1 (MQ=255) gcgcAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATAtct > 1:458722/1‑55 (MQ=255) gcgcAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGaa < 1:2802602/71‑1 (MQ=255) cgcAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTccacc > 1:862681/1‑59 (MQ=255) cgcAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTccacc > 1:3417120/1‑59 (MQ=255) cgcAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGaaa < 1:2046764/71‑1 (MQ=255) aCGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAAt < 1:3766773/69‑1 (MQ=255) cgcgGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGaaaa > 1:2115862/1‑67 (MQ=255) gcggcgGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGcc < 1:3377699/71‑1 (MQ=255) ggcggTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGa < 1:1252446/71‑1 (MQ=255) gcggTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGa > 1:1259417/1‑70 (MQ=255) gcggTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGa > 1:1466849/1‑70 (MQ=255) gcggTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGa < 1:1812781/70‑1 (MQ=255) tAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGat < 1:1140984/37‑1 (MQ=255) aaGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTc > 1:3770276/1‑49 (MQ=255) aaGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTc > 1:3533349/1‑49 (MQ=255) aaGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGAtattat > 1:583220/1‑70 (MQ=255) aaGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGAtattat > 1:1877425/1‑70 (MQ=255) aGTGGTAGATAACGTGGTGCAGACTATGCGTGATATCTCCACCAGTTCGCAGAAAATCGCCGatat > 1:429082/1‑66 (MQ=255) aGTGGTAGATAACGTGGTGCAGACTATGCGTGATATCTCCACCAGTTCGCAGAAAATCGCCGatat > 1:1785290/1‑66 (MQ=255) gTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCg > 1:2224756/1‑70 (MQ=255) gTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:78546/71‑1 (MQ=255) aGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGAtattat > 1:1872734/1‑63 (MQ=255) aGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGAtattat > 1:821497/1‑63 (MQ=255) gATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGaa < 1:1327442/47‑1 (MQ=255) gATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGc > 1:3364210/1‑66 (MQ=255) tAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:1611491/66‑1 (MQ=255) tAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:3502493/66‑1 (MQ=255) tAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCAGATATTATCAGCGTAAtt > 1:3451745/1‑70 (MQ=255) aaCGTGGTGCAGACTATGGGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGa > 1:312540/1‑71 (MQ=255) aaCGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGa > 1:3245621/1‑71 (MQ=255) aCGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAAtt < 1:2602787/68‑1 (MQ=255) aCGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGAc > 1:3354751/1‑71 (MQ=255) tggtgCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCg < 1:2916975/60‑1 (MQ=255) tggtgCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCg < 1:2606687/60‑1 (MQ=255) ggtgCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGcc < 1:2119906/46‑1 (MQ=255) gtgCAGACTATGCGTGATATTTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:567889/59‑1 (MQ=38) gtgCAGACTATGCGTGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:3707658/59‑1 (MQ=255) gtgCAGACTATGCGTGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGt < 1:790464/59‑1 (MQ=255) cAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGc > 1:2024536/1‑71 (MQ=255) tGCGCTATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAAt > 1:2104720/1‑52 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAAt > 1:1201884/1‑52 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGcc > 1:393095/1‑65 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGcc > 1:2414788/1‑65 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTcc < 1:3679471/69‑1 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTc > 1:3179336/1‑68 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTCCAg < 1:3608430/71‑1 (MQ=255) tGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTCCAg < 1:2646814/71‑1 (MQ=255) cgcgATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGc < 1:2000465/57‑1 (MQ=255) cgcgATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGc < 1:427130/57‑1 (MQ=255) cgcgATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGc < 1:1431113/57‑1 (MQ=255) cgATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTCCAGACCa < 1:1080973/71‑1 (MQ=255) | TGGCGTTAAGTGCTTCTGAAACGGCGCAACGCGGCGGTAAAGTGGTAGATAACGTGGTGCAGACTATGCGCGATATCTCCACCAGTTCGCAGAAAATCGCCGATATTATCAGCGTAATTGACGGCATTGCCTTCCAGACCA > W3110S.gb/4597324‑4597464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |