| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 430,884 | G→A | 7.7% | F118F (TTC→TTT) | tsx ← | nucleoside channel, receptor of phage T6 and colicin K |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 430,884 | 0 | G | A | 7.7% | 229.9 / 6.4 | 78 | F118F (TTC→TTT) | tsx | nucleoside channel, receptor of phage T6 and colicin K |
| Reads supporting (aligned to +/- strand): ref base G (35/37); new base A (3/3); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
GCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAA > W3110S.gb/430815‑430953 | gCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCg > 1:3597762/1‑70 (MQ=255)gCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCg > 1:1644421/1‑70 (MQ=255)gCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGa < 1:2877558/71‑1 (MQ=255) ccATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCt < 1:1135120/71‑1 (MQ=255) ccATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCt < 1:3415242/71‑1 (MQ=255) cATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCt > 1:2596360/1‑70 (MQ=255) cATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTa < 1:2707744/71‑1 (MQ=255) cATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTa < 1:17698/71‑1 (MQ=255) tCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAg > 1:2860673/1‑71 (MQ=255) tCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAg > 1:3651079/1‑71 (MQ=255) ttattaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt > 1:2360529/1‑70 (MQ=255) ttattaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt > 1:2963849/1‑70 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTa < 1:2264647/63‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt < 1:1086547/67‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt < 1:2676407/67‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt < 1:3254698/67‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt < 1:3796520/67‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg < 1:776118/70‑1 (MQ=255) ttaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg < 1:3776494/70‑1 (MQ=255) aCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCa > 1:2368685/1‑67 (MQ=255) aCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCa > 1:2822113/1‑67 (MQ=255) gACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTAtt > 1:1794811/1‑70 (MQ=255) gACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTg < 1:2560377/71‑1 (MQ=255) cccATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGt < 1:2110693/71‑1 (MQ=255) gTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTc < 1:1664783/57‑1 (MQ=255) tCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTag > 1:1214339/1‑51 (MQ=255) cGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg > 1:3297281/1‑57 (MQ=255) cGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg > 1:1491453/1‑57 (MQ=255) gTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTg < 1:3683807/70‑1 (MQ=255) gTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGt > 1:634894/1‑71 (MQ=255) gTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGt > 1:1168258/1‑71 (MQ=255) tAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAg < 1:311610/65‑1 (MQ=255) aaaTGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTc > 1:2857941/1‑70 (MQ=255) aaaTGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTc > 1:699138/1‑70 (MQ=255) aaaTGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTc > 1:923218/1‑70 (MQ=255) tGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa > 1:154884/1‑69 (MQ=255) tGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATg < 1:512619/71‑1 (MQ=255) tAGTTGTTCGCGAAGTACCACTCTTTGAACTGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATg > 1:2923445/1‑69 (MQ=255) tAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATg > 1:819063/1‑69 (MQ=255) tAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATg > 1:1800512/1‑69 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga < 1:2403322/71‑1 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga < 1:3241660/71‑1 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga < 1:3038494/71‑1 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa < 1:2565535/65‑1 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa < 1:922301/65‑1 (MQ=255) gttgttCGCGAAGTACCACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa < 1:1428912/65‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg < 1:2378082/46‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAg < 1:2937207/46‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTg < 1:3532931/51‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTg < 1:1344032/51‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCt < 1:2722068/58‑1 (MQ=255) ttgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGaa < 1:540600/71‑1 (MQ=255) gttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAAc > 1:3466214/1‑71 (MQ=255) gttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAAc > 1:1480538/1‑71 (MQ=255) ttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga < 1:2960891/67‑1 (MQ=255) cgcgAAGTACCACTCTTTGAACGGACCGAAGCTAAAGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGt < 1:1128284/70‑1 (MQ=255) cgcgAAGTAACACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTg > 1:2937628/1‑71 (MQ=255) gcgAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTgg < 1:1843837/71‑1 (MQ=255) gcgAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTg < 1:2910464/70‑1 (MQ=255) aaGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTc > 1:1126748/1‑71 (MQ=255) aaGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTc > 1:2508578/1‑71 (MQ=255) aaGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTAGTCGATGGAGAAACg > 1:3531939/1‑65 (MQ=255) aCCACTCTTTGAACGGACCGAAGCTAAGCTCAGTATTGGTCAGCt > 1:3525898/1‑45 (MQ=255) ccACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGgaga > 1:457620/1‑56 (MQ=255) ccACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGAttt > 1:1626479/1‑71 (MQ=255) ccACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGAttt > 1:2447689/1‑71 (MQ=255) ccACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGAttt > 1:2345441/1‑71 (MQ=255) ccACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGAtt < 1:1042420/70‑1 (MQ=255) ctctTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTc < 1:2105029/45‑1 (MQ=255) ctctTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCg < 1:389040/46‑1 (MQ=255) tctTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAg < 1:2773910/38‑1 (MQ=255) ctTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa > 1:1493811/1‑45 (MQ=255) tttGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:2164514/1‑62 (MQ=255) tttGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATa > 1:770225/1‑70 (MQ=255) tttGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATa > 1:3853829/1‑70 (MQ=255) tGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATa < 1:884286/68‑1 (MQ=255) aCGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATg < 1:3411882/41‑1 (MQ=255) cGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGa > 1:3310960/1‑38 (MQ=255) aCCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGc > 1:218836/1‑67 (MQ=255) cGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGaa > 1:40689/1‑71 (MQ=255) | GCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAA > W3110S.gb/430815‑430953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |