| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 436,250 | C→T | 12.2% | I146I (ATC→ATT) | pgpA → | phosphatidylglycerophosphatase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 436,250 | 0 | C | T | 12.2% | 126.8 / 7.7 | 49 | I146I (ATC→ATT) | pgpA | phosphatidylglycerophosphatase A |
| Reads supporting (aligned to +/- strand): ref base C (21/22); new base T (4/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCAC > W3110S.gb/436183‑436307 | atatGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgac < 1:1552887/71‑1 (MQ=255) tgtgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:2441151/70‑1 (MQ=255) tgtgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:1260081/70‑1 (MQ=255) tgtgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGat < 1:3323104/71‑1 (MQ=255) gtgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:3034693/69‑1 (MQ=255) tgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGatat > 1:2359885/1‑71 (MQ=255) tgGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGatat > 1:173031/1‑71 (MQ=255) gAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATAtt < 1:3156763/70‑1 (MQ=255) gAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATAtt < 1:2151609/70‑1 (MQ=255) ccGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGc > 1:1616094/1‑71 (MQ=255) ccgctccgctGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGt > 1:1576412/1‑71 (MQ=255) ccGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGt > 1:293759/1‑71 (MQ=255) ccGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGt > 1:690136/1‑71 (MQ=255) gATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:1874970/54‑1 (MQ=255) gATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:2645513/54‑1 (MQ=255) gATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATcgacga < 1:3783038/54‑1 (MQ=255) gATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGa > 1:3517142/1‑71 (MQ=255) aTCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCgg < 1:1252554/65‑1 (MQ=255) aTCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGAt < 1:2074440/71‑1 (MQ=255) cctcTGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTGACGATATTGTCGCCGGGGTGAt < 1:3488890/66‑1 (MQ=255) ccGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCggg > 1:1943827/1‑64 (MQ=255) ccGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCgg > 1:2548833/1‑63 (MQ=255) ccGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGAACATGATCGACGATATTGTCGCCGGGGTGAt > 1:2877369/1‑69 (MQ=255) ccGCTGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTGACGATATTGTCGCCGGGGTGAt < 1:3120539/69‑1 (MQ=255) cTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCggg < 1:1244088/61‑1 (MQ=255) cTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCg < 1:3676262/71‑1 (MQ=255) tGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTGACGATATTGTCg > 1:1456380/1‑55 (MQ=255) tGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTGACGATATTGTCGc > 1:3652166/1‑56 (MQ=255) tGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTGACGATATTGTCGc > 1:992397/1‑56 (MQ=255) tGGTTTGATCGCAATGTACATGGCGGCATGGGGATCATGATTAACGATATTGTCGc > 1:960356/1‑56 (MQ=255) tGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCa > 1:3384376/1‑71 (MQ=255) aTCGCAATGTGCATGGCGGCATGGGGATCCTGATCGACGATAtt < 1:3459997/44‑1 (MQ=255) aTCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATc < 1:1968557/71‑1 (MQ=255) aTGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCg < 1:2773662/57‑1 (MQ=255) aTGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCg < 1:2682461/57‑1 (MQ=255) tgCATGGCGGCATGGGGATCATGATCGACGATAtt > 1:3261602/1‑35 (MQ=255) tgCATGGCGGCATGGGGATCATGATCGACGATAtt > 1:1558567/1‑35 (MQ=255) cATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTa < 1:3433998/70‑1 (MQ=255) cATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTa < 1:651459/70‑1 (MQ=255) tGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGt < 1:3254182/42‑1 (MQ=255) tGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGt < 1:345392/42‑1 (MQ=255) tGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATc < 1:1763642/58‑1 (MQ=255) ggcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCATGTATTTTATCgg > 1:1899373/1‑71 (MQ=255) gcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGt > 1:2089676/1‑71 (MQ=255) gcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGtcat < 1:1740284/71‑1 (MQ=255) aTGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGtcatca < 1:967468/71‑1 (MQ=255) gggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCg > 1:821572/1‑61 (MQ=255) gggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCAc > 1:3853186/1‑69 (MQ=255) ggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGtc > 1:2774530/1‑63 (MQ=255) gATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTAt > 1:1011874/1‑46 (MQ=255) gATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTAt > 1:3371960/1‑46 (MQ=255) gATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCAGTa > 1:3569840/1‑45 (MQ=255) aTCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTAt < 1:258947/50‑1 (MQ=255) aTCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTAt < 1:3375732/50‑1 (MQ=255) aTCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTAt < 1:2301899/50‑1 (MQ=255) aTCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCgg < 1:2368652/53‑1 (MQ=255) | ATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCAC > W3110S.gb/436183‑436307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |