| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 138.2 / NA | 42 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/19); total (23/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG > W3110S.gb/547627‑547761 | ttCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc < 1:1423523/71‑1 (MQ=255) gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:3150954/70‑1 (MQ=255) tcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa < 1:3274939/71‑1 (MQ=255) cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac < 1:3043620/71‑1 (MQ=255) cGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCt > 1:627749/1‑71 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:3636612/71‑1 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa > 1:2626704/1‑70 (MQ=255) acacTGGCTGGATCTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg > 1:863739/1‑71 (MQ=255) cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa < 1:1680530/58‑1 (MQ=255) acTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc < 1:2976070/68‑1 (MQ=255) tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAggg > 1:786162/1‑50 (MQ=255) tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACACCGGgcc > 1:191336/1‑70 (MQ=255) gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:11495/1‑46 (MQ=255) gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:125530/1‑46 (MQ=255) cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:2935795/1‑43 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:3390449/49‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt < 1:1675247/71‑1 (MQ=255) cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:1508026/1‑39 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg < 1:1564232/68‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:1323184/1‑68 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:264903/70‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGAGCTGGCAGga < 1:2232115/70‑1 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc < 1:2175107/58‑1 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:1133812/1‑54 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc > 1:845243/1‑57 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag < 1:2949702/68‑1 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc > 1:1844411/1‑58 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg > 1:3828019/1‑44 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:913193/1‑71 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc > 1:1365951/1‑43 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:3312449/56‑1 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga > 1:2529583/1‑56 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg < 1:827649/71‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:708965/1‑66 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:3179226/71‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:2684172/71‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:1778086/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa > 1:1979995/1‑55 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa > 1:1975688/1‑55 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa > 1:3735162/1‑60 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:2118528/1‑50 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg > 1:3172687/1‑71 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg > 1:3779172/1‑71 (MQ=255) | TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG > W3110S.gb/547627‑547761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |