| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 553,023 | A→G | 16.7% | I47I (ATT→ATC) | lpxH ← | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 553,023 | 0 | A | G | 16.7% | 62.1 / 6.7 | 30 | I47I (ATT→ATC) | lpxH | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base A (15/10); new base G (3/2); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAA > W3110S.gb/552955‑553089 | ccGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAAt > 1:2104609/1‑71 (MQ=255) aTCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATg > 1:743152/1‑71 (MQ=255) aTCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATg > 1:2250739/1‑71 (MQ=255) acacCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCa < 1:3441602/71‑1 (MQ=255) tacCGCTTTGATTGCTGCGGCCATCTGGTGATGAAGTGGGTTGGGATCGTCGTCGCCGATCCAGGCTTCa > 1:700069/2‑70 (MQ=255) tacCGCTTTGATTGCTGCGGCCATCTGGTGATGAAGTGGGTTGGGATCGTCGTCGCCGATCCAGGCTTCa > 1:380728/2‑70 (MQ=255) tacCGCTTTGATTGCTGCGGCCATCTGGTGATGAAGTGGGTTGGGATCGTCGTCGCCGATCCAGGCTTCa > 1:2131296/2‑70 (MQ=255) cacCGCTTTGAGCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCa < 1:1702986/70‑1 (MQ=255) ccGCTTTGATTGCTGCGGCCATCTGGTGATGAAGTGGGTTGGGATCGTCGTCGCCGATCCAGGCTTCAAAc < 1:1991868/71‑1 (MQ=255) ccGCTTTGATTGCTGCGGCCATCTGGTGATGAAGTGGGTTGGGATCGTCGTCGCCGATCCAGGCTTCAAAc < 1:141476/71‑1 (MQ=255) ccGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCaaa > 1:1629352/1‑70 (MQ=255) cGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACa < 1:1679859/71‑1 (MQ=255) cGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACa < 1:235084/71‑1 (MQ=255) gCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCaaa > 1:1808479/1‑68 (MQ=255) tGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCg > 1:16782/1‑71 (MQ=255) gATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCAATGCTACAAACAGATCGc > 1:3305373/1‑71 (MQ=255) ccgccATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAata < 1:2444161/71‑1 (MQ=255) tCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACa < 1:1541132/69‑1 (MQ=255) ttGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAata > 1:3773586/1‑63 (MQ=255) ttGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAgcgc > 1:168845/1‑71 (MQ=255) gCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGt > 1:2878433/1‑71 (MQ=255) tGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAAtata > 1:2194525/1‑59 (MQ=255) tGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTg < 1:1197391/70‑1 (MQ=255) tGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGcgcg > 1:1967935/1‑56 (MQ=255) gtcgtcgCCAATCCAAGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTg > 1:1021664/1‑58 (MQ=255) tcgtcgCCAATCCATGCTTCAAACAGATCGCCAAGaa < 1:1850897/37‑1 (MQ=255) tcgtcgCCAATCCATGCTTCAAACAGAGCGCCAATAATATACAGCGCGTCGGCCTTg < 1:3431283/57‑1 (MQ=255) gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACa > 1:871631/1‑41 (MQ=255) gCCAATCCATGCTTCAAACAGATCGCCAAGAATATAc > 1:362575/1‑37 (MQ=255) gCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTaaa < 1:2204911/70‑1 (MQ=255) | CCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAA > W3110S.gb/552955‑553089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |