Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 644,865 A→T 8.0% V313V (GTT→GTA)  citT ← citrate:succinate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb644,8650AT 8.0% 199.4 / 7.5 75V313V (GTT→GTA) citTcitrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base A (28/41);  new base T (4/2);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATAGCGGGTAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATC  >  W3110S.gb/644797‑644934
                                                                    |                                                                     
ttATAGCGGGTAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAAc                                                                     >  1:3595408/1‑71 (MQ=255)
   taGCGGGTAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGc                                                                  <  1:1009262/71‑1 (MQ=255)
   taGCGGGTAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGc                                                                  <  1:798445/71‑1 (MQ=255)
          tAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCa                                                           >  1:2300276/1‑71 (MQ=255)
           aaTGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCAt                                                          >  1:2959652/1‑71 (MQ=255)
            aTGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCAtt                                                         <  1:2058969/71‑1 (MQ=255)
            aTGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCAt                                                          >  1:3108484/1‑70 (MQ=255)
             tGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCg                                                                   <  1:951539/60‑1 (MQ=255)
             tGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCg                                                                   <  1:3181999/60‑1 (MQ=255)
             tGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCg                                                                   <  1:1527605/60‑1 (MQ=255)
              gTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAg                                                             <  1:3216945/65‑1 (MQ=255)
              gTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAg                                                             <  1:1533156/65‑1 (MQ=255)
              gTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAg                                                             <  1:840929/65‑1 (MQ=255)
              gTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTaa                                                       >  1:3420243/1‑71 (MQ=255)
              gTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACAGCAGTAGCATTaa                                                       >  1:1028960/1‑71 (MQ=255)
               tCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:1513771/69‑1 (MQ=255)
               tCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAAt                                                      >  1:3359391/1‑71 (MQ=255)
                  ttCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGAc                                                   >  1:2980323/1‑71 (MQ=255)
                   tCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATg                                                     <  1:1244768/68‑1 (MQ=255)
                   tCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGa                                                    <  1:1375596/69‑1 (MQ=255)
                   tCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGAc                                                   <  1:1033641/70‑1 (MQ=255)
                   tCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACt                                                  >  1:125048/1‑71 (MQ=255)
                     cAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACtt                                                 <  1:1374010/70‑1 (MQ=255)
                     cAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACtt                                                 <  1:3504344/70‑1 (MQ=255)
                     cAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACtt                                                 <  1:12137/70‑1 (MQ=255)
                       aGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCAc                                              >  1:2441453/1‑71 (MQ=255)
                       aGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCAc                                              >  1:3051648/1‑71 (MQ=255)
                         gCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACtt                                                 <  1:875396/66‑1 (MQ=255)
                         gCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTc                                                <  1:358861/67‑1 (MQ=255)
                         gCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTc                                                <  1:3785503/67‑1 (MQ=255)
                           acaACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGc                                           >  1:1853068/1‑70 (MQ=255)
                            caACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:3566043/56‑1 (MQ=255)
                            caACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:1589145/56‑1 (MQ=255)
                            caACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:2446301/56‑1 (MQ=255)
                            caACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:2091824/56‑1 (MQ=255)
                            caACGTGCAGGGCCAGCA‑TAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTc                                                <  1:3602383/63‑1 (MQ=255)
                             aaCGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCaa                                        <  1:2289927/71‑1 (MQ=255)
                               cGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTaa                                                       >  1:603690/1‑54 (MQ=255)
                               cGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTaa                                                       >  1:104869/1‑54 (MQ=255)
                                 tGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:815445/51‑1 (MQ=255)
                                 tGCAGGGCAAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:1457067/51‑1 (MQ=255)
                                   cAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGa                                                    >  1:1709053/1‑53 (MQ=255)
                                   cAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGa                                                    >  1:3357424/1‑53 (MQ=255)
                                      ggCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCa                                         <  1:3572247/61‑1 (MQ=255)
                                       gCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCcaca                               >  1:3044600/1‑70 (MQ=255)
                                       gCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCcaca                               >  1:560738/1‑70 (MQ=255)
                                        ccAGCATTAGCGAAACTGCCAGCAGGCCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACaa                              <  1:3635972/70‑1 (MQ=255)
                                        ccAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACaaa                             >  1:565097/1‑71 (MQ=255)
                                           gCATTAGCGAAACTGCCAGCAGACCAACCGCAGTTGCATTa                                                        <  1:491564/41‑1 (MQ=255)
                                           gCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTa                                                        <  1:1869730/41‑1 (MQ=255)
                                            cATCAGCGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCt                          >  1:1150190/1‑70 (MQ=255)
                                            cATCAGCGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCt                          >  1:919338/1‑70 (MQ=255)
                                            cATCAGCGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCt                          >  1:587573/1‑70 (MQ=255)
                                            cATCAGCGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCt                          >  1:1393990/1‑70 (MQ=255)
                                             aTTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCaaa                                       >  1:2857429/1‑56 (MQ=255)
                                               tAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCa                                         <  1:3341372/52‑1 (MQ=255)
                                               tAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAAc                            <  1:3463497/65‑1 (MQ=255)
                                                 gCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCt                     <  1:1857801/70‑1 (MQ=255)
                                                  cGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGa                   <  1:1223366/71‑1 (MQ=255)
                                                  cGAAACTGCCAGCAGACCTACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGa                   <  1:692288/71‑1 (MQ=255)
                                                    aaaCTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTc                                                <  1:3810496/40‑1 (MQ=255)
                                                    aaaCTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTc                                                <  1:537514/40‑1 (MQ=255)
                                                          ccAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATAc            <  1:608537/70‑1 (MQ=255)
                                                           cagcagACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACaa          <  1:265112/71‑1 (MQ=255)
                                                           cagcagACCAACCGCAGTACCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACaa          <  1:1398617/71‑1 (MQ=255)
                                                            agcagACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCca                                 >  1:1593233/1‑47 (MQ=255)
                                                             gcagACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACaaa                             >  1:2539929/1‑50 (MQ=255)
                                                                gACCAACCGCAGTAGCATTAATGACTTCACTGCCaaa                                       >  1:2613160/1‑37 (MQ=255)
                                                                  ccAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAAt   >  1:2446109/1‑71 (MQ=255)
                                                                  ccAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAAt   >  1:3706900/1‑71 (MQ=255)
                                                                  ccAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAAt   >  1:3657028/1‑71 (MQ=255)
                                                                   cAACCGCAGTAGCATTAATGACTTCACTGCCAAATAGCCACAAACCTAAGCTGAGCAATACAAGGCCAATc  <  1:562232/71‑1 (MQ=255)
                                                                   cAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAgg        >  1:2099953/1‑65 (MQ=255)
                                                                   cAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAAt   >  1:2566768/1‑70 (MQ=255)
                                                                   cAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATc  <  1:1428041/71‑1 (MQ=255)
                                                                    aaCCGCAGTAGCATTAATGACTTCACTGCCAAATAccc                                  >  1:565722/1‑38 (MQ=255)
                                                                    aaCCGCAGTAGCATTAATGACTTCACTGCCAAATAccc                                  >  1:1865305/1‑38 (MQ=255)
                                                                    aaCCGCAGTAGCATTAATGACTTCACTGCCAAATAccc                                  >  1:1489072/1‑38 (MQ=255)
                                                                    |                                                                     
TTATAGCGGGTAATGTCTTTCCAAGGCACAACGTGCAGGGCCAGCATTAGCGAAACTGCCAGCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATC  >  W3110S.gb/644797‑644934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑