| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 670,276 | A→C | 11.5% | L18L (CTT→CTG) | cobC ← | predicted alpha‑ribazole‑5'‑P phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 670,276 | 0 | A | C | 11.5% | 106.6 / 11.9 | 52 | L18L (CTT→CTG) | cobC | predicted alpha‑ribazole‑5'‑P phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base C (4/2); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
TGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGA > W3110S.gb/670214‑670346 | tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATc > 1:3335330/1‑71 (MQ=255)tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATc < 1:1450066/71‑1 (MQ=255)tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATc > 1:3654181/1‑71 (MQ=255) cAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGa > 1:289362/1‑71 (MQ=255) gATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCa < 1:2104901/65‑1 (MQ=255) tttGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTc > 1:3706649/1‑71 (MQ=255) ttGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCTCATGACCGCTGTAAAGACCATCGATATTc > 1:941193/1‑70 (MQ=255) ttGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTc > 1:2605643/1‑70 (MQ=255) gcgcTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCt > 1:1487757/1‑71 (MQ=255) gcTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAATACCATCGATATTCGCt < 1:3113738/69‑1 (MQ=255) ttGCTCAATACCGCGCGCGGTCAGGGGGGTGGGGGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCg > 1:101836/1‑71 (MQ=255) gCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGata > 1:1439691/1‑59 (MQ=255) aaTACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTc < 1:2706783/69‑1 (MQ=255) aaTACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCAc < 1:301933/71‑1 (MQ=255) tACCGCGCGCGGTCAGGGTGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTc > 1:686517/1‑67 (MQ=255) tACCGCGCGCGGTCAGGGGTGTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGtt > 1:2341359/1‑65 (MQ=255) tACCGCGCGCGGTCAGGGGTGTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGTTTCAcaa > 1:2539449/1‑69 (MQ=255) tACCGCGCGCGGTCAGGGGTGTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGTTTCACCa > 1:14460/1‑71 (MQ=255) tACCGCGCGCGGTCAGGGGTGTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGTTTCACCa > 1:2376128/1‑71 (MQ=255) ccGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCAt > 1:2638779/1‑70 (MQ=255) ccGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCAt > 1:1217987/1‑70 (MQ=255) ccGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCCCTTGCGTTTCACCAt > 1:3422796/1‑70 (MQ=255) ccGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATAGATATTCGCTTGCGTTTCACCAt > 1:3258190/1‑70 (MQ=255) cgcgcgGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACg < 1:1774159/71‑1 (MQ=255) cgGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAAtt < 1:507740/71‑1 (MQ=255) cgGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAAtt < 1:3313006/71‑1 (MQ=255) cgGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAAt < 1:3693912/70‑1 (MQ=255) tCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGa < 1:2367696/39‑1 (MQ=255) cAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAAc > 1:1783570/1‑70 (MQ=255) aGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACca > 1:1117042/1‑71 (MQ=255) gggtgTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGTTTCACCATGACGAATTAACcaca < 1:1983816/71‑1 (MQ=255) gggtgTGGGCGCATGACCGCTGTACAGACCATCGACATTCGCTTGCGTTTCACCATGACGAATTAACcaca < 1:3453196/71‑1 (MQ=255) ggggTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAg < 1:3800061/71‑1 (MQ=255) gTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCg < 1:1565210/71‑1 (MQ=255) ggCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcat > 1:2360725/1‑71 (MQ=255) cgcATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatca < 1:1848317/71‑1 (MQ=255) aCCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCt > 1:485875/1‑71 (MQ=255) aCCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCt > 1:58817/1‑71 (MQ=255) aCCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCt > 1:1170282/1‑71 (MQ=255) gCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcat < 1:660887/60‑1 (MQ=255) cTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCg > 1:577512/1‑70 (MQ=255) cTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCg > 1:1135903/1‑70 (MQ=255) cTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCg > 1:2516164/1‑70 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACg < 1:462952/39‑1 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc < 1:3322816/55‑1 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc < 1:3651249/55‑1 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc < 1:3211982/55‑1 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc < 1:1370783/55‑1 (MQ=255) gTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc < 1:794423/55‑1 (MQ=255) tAAAGACCATCGATATTCGCTTGCGTTTCACCATGACg < 1:997286/38‑1 (MQ=255) tAAAGACCATCGATATTCGCTTGCGTTTCACCATGACg < 1:1323837/38‑1 (MQ=255) tAAAGACCATCGATATTCGCTTGCGTTTCACCATGACg < 1:2330246/38‑1 (MQ=255) aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatca > 1:2930891/1‑56 (MQ=255) aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGa > 1:1229764/1‑71 (MQ=255) aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGa > 1:1609917/1‑71 (MQ=255) aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGa > 1:1883955/1‑71 (MQ=255) aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGa > 1:2972429/1‑71 (MQ=255) | TGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGA > W3110S.gb/670214‑670346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |